MERCURIUS™
Organoid DRUG-seq
Multiplexed extraction-free library preparation kits for Illumina®
RNA-seq library preps directly from organoid lysis without prior RNA isolation.
Catalog number | #10861 | #11061 | #10871 | #11071 |
Total preps How many library preps can be prepared in total with one kit | 96 | 384 | 384 | 1'536 |
RNA and plate multiplexing format How many samples can be multiplexed in one single tube | 96 | 96 | 384 | 384 |
Barcoded oligo-dT plates included | 1 | 4 | 1 | 4 |
UDI pairs included How many individual libraries can be sequenced together | 4 | 4 | 4 | 4 |
Documentation |
Cat ##10861 | ||||
---|---|---|---|---|
Total reactions How many library preps can be prepared in total with one kit | ||||
RNA multiplexing format How many library preps can be prepared in total with one kit | 96 | |||
UDI pairs included How many library preps can be prepared in total with one kit | 4 | |||
Cat ##11061 | ||||
---|---|---|---|---|
Total reactions How many library preps can be prepared in total with one kit | ||||
RNA multiplexing format How many library preps can be prepared in total with one kit | 96 | |||
UDI pairs included How many library preps can be prepared in total with one kit | 4 | |||
Cat ##10871 | ||||
---|---|---|---|---|
Total reactions How many library preps can be prepared in total with one kit | 384 | |||
RNA multiplexing format How many library preps can be prepared in total with one kit | 384 | |||
UDI pairs included How many library preps can be prepared in total with one kit | 4 | |||
Cat ##11071 | ||||
---|---|---|---|---|
Total reactions How many library preps can be prepared in total with one kit | ||||
RNA multiplexing format How many library preps can be prepared in total with one kit | 384 | |||
UDI pairs included How many library preps can be prepared in total with one kit | 4 | |||
Add-ons
Accessories
Organoid lysis
module
- Optimized lysis buffer for complete lysis and efficient reverse transcription.
- Compatible with DRUG-seq library prep kits.
Accessories
UDI expansion
module
- Compatible with the MERCURIUS™ product line.
- 12 UDI pairs.
- Flexible dual indexing solutions.
Package
Libraries
sequencing
$3
per million reads
- Take advantage of our low sequencing price, which is enabled by the recurrent sequencing runs.
- Your libraries will be sequenced in large sequencing runs with other compatible BRB-seq and DRUG-seq libraries.
Package
Data
pre-processing
$600 NOW $150
per 96 samples
- We provide you with everything from raw data to gene count matrices and sequencing report.
- Guaranteed turn-on time: 2 days.
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Benefits
The MERCURIUS™ Extraction-free Organoid DRUG-seq kits contain all the oligos and enzymes needed to go from 3D organoids and spheroids to sequencing-ready DNA libraries
Ideal for screening projects No need for prior RNA extraction Improved DRUG-seq protocol One-day lab workflow
More samples, more replicates. Robust results, significant discoveries.
An optimized lysis buffer for complete lysis and efficient reverse transcription.
Without pre-amplification, leading to higher mapping and gene detection rates.
Convenient and short protocol. From samples to sequencing-ready libraries in one day.
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Experimental workflow at a glance
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Product specifications
For (application)
3' mRNA sequencing
For use with (equipment)
Illumina NGS instruments
Species compatibility
All eukaryotic species
Available formats
96, 384, 1'536, 6'144 and 24'576 reactions
Shipping conditions
Dry ice
Storage conditions
-20C
Sample dataset
-
Prostate cancer
Number of samples:12Reads per sample in demo dataset:10'000 readsTo have access to the deep-sequenced dataset (24.5M reads per sample) contact us.Demo dataset file size:7.16 MB
FAQs
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Each BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.
To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
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The recommended range of input material is in the range of 5’000-50’000 cells.
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The only difference between DRUG-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the DRUG-seq barcode sequence.
For a thorough description of DRUG-seq data processing, please refer to the DRUG-seq kit user guide.
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The barcode set for your kit is conveniently located on the kit label. Please refer to the label for accurate identification.
For optimal compatibility, ensure that you use the appropriate plate format (e.g., for kits designed for 96 reactions, the 96 well-plate format should be used). This ensures accurate and efficient processing of your samples. If you have any further questions or concerns, please contact our support team for assistance by email or using our live chat tool.
Speak with our RNA sequencing experts
Book a one-on-one call with one of our RNA experts to discover how we can assist your next project.