How does it work
The easy-to-use cloud-based BRB-seq pipeline greatly facilitates the handling of raw BRB-seq data.
Simply upload your fastq files and press a button, it's that simple!
The pipeline allows the user to align BRB-seq data to the genome of choice and produces:
- a report file displaying the main alignment statistics (e.g. mapping rate and number of detected genes).
- a gene count matrix that can be easily used for offline gene expression analysis.
From 5 mins to 20 mins depending on file size
Upload raw read1 and read2.fastq files
Upload sample info in .xls format
Select genome of interest
From 20 min to 24 hours depending on .fastq and genome size
- Gene count matrix
- UMI count matrix
- PDF QC report file with individual sample info:
- Number of raw reads/sample
- Alignment stats
- Number of detected genes
- UMI duplication levels