MERCURIUS™
DRUG-seq kits
Multiplexed extraction-free library preparation kits
for Illumina®, AVITI™ and MGI*
RNA-seq library preps directly from cell lysates without prior RNA isolation.
* For native kits' compatibility with MGI sequencers, contact us.
Catalog number | #10841 | #11041 | #10851 | #11051 |
Total preps
|
96 | 384 | 384 | 1'536 |
RNA and plate multiplexing format
|
96 | 96 | 384 | 384 |
Barcoded oligo-dT plates included
| 1 | 4 | 1 | 4 |
UDI pairs included
|
4 | 4 | 4 | 4 |
Documentation |
Cat ##10841 | ||||
---|---|---|---|---|
Total reactions
|
96 | |||
RNA multiplexing format
|
96 | |||
UDI pairs included
|
4 | |||
Cat ##11041 | ||||
---|---|---|---|---|
Total reactions
|
384 | |||
RNA multiplexing format
|
96 | |||
UDI pairs included
|
4 | |||
Cat ##10851 | ||||
---|---|---|---|---|
Total reactions
|
384 | |||
RNA multiplexing format
|
384 | |||
UDI pairs included
|
4 | |||
Cat ##11051 | ||||
---|---|---|---|---|
Total reactions
|
||||
RNA multiplexing format
|
384 | |||
UDI pairs included
|
4 | |||
Add-ons
Accessories
Cell lysis
module
- Optimized lysis buffer for complete lysis and efficient reverse transcription.
- Compatible with DRUG-seq library prep kits.
Accessories
UDI expansion
module
- Compatible with the MERCURIUS™ product line.
- 12 UDI pairs.
- Flexible dual indexing solutions.
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Benefits
The MERCURIUS™ Extraction-free DRUG-seq kits contain all the oligos and enzymes needed to go from 2D cell cultures to sequencing-ready libraries.
Ideal for screening projects No need for prior RNA extraction Improved DRUG-seq protocol One-day lab workflow
More samples, more replicates. Robust results, significant discoveries.
An optimized lysis buffer for complete lysis and efficient reverse transcription.
Without pre-amplification, leading to higher mapping and gene detection rates.
Convenient and short protocol from samples to sequencing-ready libraries in one day.
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Experimental workflow at a glance
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Performance
1. Detection of 12'000+ genes at 1.5 million reads per sample across 384 samples
Distribution of the number of detected genes across 384 samples prepared with the MERCURIUS™ DRUG-seq library preparation kit. The library was sequenced at an average of 1.5 million reads per sample on an Illumina NovaSeq 6000.
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Product specifications
For (application)
3' mRNA sequencing
For use with (equipment)
Illumina and AVITI NGS instruments
Species compatibility
All eukaryotic species
Available formats
96, 384, 1'536, 6'144 and 24'576 preps
Shipping conditions
Dry ice
Storage conditions
-20C
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Validated cell lines
Cell line Specie Tissue Culture type A375 h.sapiens Skin, malignant melanoma adherent GM12878 h.sapiens PBMC, lymphoblastoid suspension H295R h.sapiens Adrenal gland, carcinoma adherent HAP1 h.sapiens KBM-7 derived, chronic
myelogenous leukemiaadherent hASC h.sapiens Patient-derived adipose stromal cells adherent hASC-Adipocytes h.sapiens Differentiated hASC adherent HEK293 h.sapiens Kidney embryonic adherent HeLa h.sapiens Cervix, adenocarcinoma adherent HepG2 h.sapiens Liver, carcinoma adherent Huh7 h.sapiens Liver, carcinoma adherent iNeurons h.sapiens Differentiated iPSC adherent MCF-7 h.sapiens Breast, adenocarcinoma adherent PANC-1 h.sapiens Pancreas, carcinoma adherent U2OS h.sapiens Bone, osteosarcoma adherent
Sample datasets
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Hap1
Number of samples:24Reads per sample in demo dataset:10'000 readsTo have access to the deep-sequenced dataset (7.3 M reads per sample) contact us.Demo dataset file size:13.3 MBHepG2
Number of samples:65Reads per sample in demo dataset:10'000 readsTo have access to the deep-sequenced dataset (3.9 M reads per sample) contact us.Demo dataset file size:4.76 MBHeLa
Number of samples:24Reads per sample in demo dataset:10'000 readsTo have access to the deep-sequenced dataset (7.1 M reads per sample) contact us.Demo dataset file size:13.6 MB
FAQs
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Each DRUG-seq or BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.
To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
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The recommended range of input material is in the range of 5’000-50’000 cells.
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The only difference between DRUG-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the DRUG-seq barcode sequence.
For a thorough description of DRUG-seq data processing, please refer to the DRUG-seq kit user guide.
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The barcode set for your kit is conveniently located on the kit label. Please refer to the label for accurate identification.
For optimal compatibility, ensure that you use the appropriate plate format (e.g., for kits designed for 96 reactions, the 96 well-plate format should be used). This ensures accurate and efficient processing of your samples. If you have any further questions or concerns, please contact our support team for assistance by email or using our live chat tool.
Speak with our RNA sequencing experts
Book a one-on-one call with one of our RNA experts to discover how we can assist your next project.