Full-length BRB-seq Services | RNA-seq Service

Multiplexed full-length mRNA-seq

Full-length mRNA-seq library prep, 96 samples in a single tube.

Full-length transcript coverage

From differential gene expression to splicing variants and isoform detection.

Fast turnaround times

From samples to data in as little as one month.

MERCURIUS ™

Full-Length BRB-seq service

The MERCURIUS™ Full-Length BRB-seq service offers a convenient and streamlined solution for full-length mRNA transcriptomics projects of any size.

Clients can send us purified RNA samples, which are always quality-checked before launching our Full-Length BRB-seq pipeline. During the process, we always keep clients informed at defined checkpoints so that we can decide together how to best proceed to the next steps.

Next generation sequencing and data pre-processing (including alignment to the genome of choice) are part of our standard service as well. As a result, we provide our clients raw data, sequencing and alignment reports, and gene count matrices which can be used for downstream gene expression analysis.

Optional downstream differential gene expression analysis are also available upon request.

Client prepares the samples
Client sends submission form
Client ships samples to Alithea
1 week

Incoming QC - Client Checkpoint

(Nanodrop, Ribogreen, Tapestation)
2 days

MERCURIUS™ Full-Length BRB-seq library preparation
1 week

Library QC - Client Checkpoint

(Qubit, Fragment analyzer, shallow sequencing)
1 week

Deep sequencing
1 week

Data analysis and reporting - Client Checkpoint
Data
Optional: Alithea performs Differential Gene Expression analysis

Performance

Uniform detection of 22'000+ genes at 12 million reads per sample across 48 samples

Distribution of the number of detected genes across 48...

Full-Length BRB-seq shows high demultiplexing rate and high mapping rate to exons

Full-Length BRB-seq performance shows 99%...

Mapping rates as high as 80% for different RNA inputs

Mapping rates are uniform across three RNA inputs...

Full-Length BRB-seq exhibits reads distribution across the transcript’s entire length

The gene body coverage shows a consistent and uniform...

>50'000 isoforms detected at 15M sequencing depth

Benchmarking the Full-Length BRB-seq protocol against...

To generate high-quality sequencing data we recommend starting with 10 - 1000 ng of total purified RNA per sample.

The total RNA amount per pool should be at least 1000 ng.
In addition to total RNA amount, it is important that the samples contain RNA of high integrity (RIN > 6) and are devoid of contaminants (Nanodrop A260/A230 between 1.8 and 2.2).
Full-Length BRB-seq provides comprehensive coverage of the full-length mRNA transcripts.

We, therefore, normally recommend sequencing 12-20 million reads for each sample, which enables the reliable and unbiased detection of over 20,000 genes.
As part of our standard service pipeline, we align the generated data to the genome of choice, provide a detailed report on the alignment and gene counting statistics and, finally, provide ready-to-use gene count matrices for downstream analysis.

Optionally, we can include differential gene expression analysis.

MERCURIUS™

Full-Length BRB-seq service

Benefits

Multiplexed full-length mRNA-seq

Full-length transcript coverage

Fast turnaround times

What we do

MERCURIUS ™

Full-Length BRB-seq service

How does it work

Client prepares the samples

Client sends submission form

Client ships samples to Alithea

Incoming QC - Client Checkpoint

MERCURIUS™ Full-Length BRB-seq library preparation

Library QC - Client Checkpoint

Deep sequencing

Data analysis and reporting - Client Checkpoint

Data

Optional: Alithea performs Differential Gene Expression analysis

Performance

Uniform detection of 22'000+ genes at 12 million reads per sample across 48 samples

Full-Length BRB-seq shows high demultiplexing rate and high mapping rate to exons

Mapping rates as high as 80% for different RNA inputs

Full-Length BRB-seq exhibits reads distribution across the transcript’s entire length

>50'000 isoforms detected at 15M sequencing depth

Uniform detection of 22'000+ genes at 12 million reads per sample across 48 samples

Full-Length BRB-seq shows high demultiplexing rate and high mapping rate to exons

Mapping rates as high as 80% for different RNA inputs

Full-Length BRB-seq exhibits reads distribution across the transcript’s entire length

>50'000 isoforms detected at 15M sequencing depth

FAQs

See our products

Speak with our RNA sequencing experts

Subscribe to our newsletter

Receive email updates on our latest products and services, news and event updates and more. No ads, no spam.

MERCURIUS™

Full-Length BRB-seq service

Benefits

Multiplexed full-length mRNA-seq

Full-length transcript coverage

Fast turnaround times

What we do

MERCURIUS ™

Full-Length BRB-seq service

How does it work

Client prepares the samples

Client sends submission form

Client ships samples to Alithea

Incoming QC - Client Checkpoint

MERCURIUS™ Full-Length BRB-seq library preparation

Library QC - Client Checkpoint

Deep sequencing

Data analysis and reporting - Client Checkpoint

Data

Optional: Alithea performs Differential Gene Expression analysis​

Performance

Uniform detection of 22'000+ genes at 12 million reads per sample across 48 samples

Full-Length BRB-seq shows high demultiplexing rate and high mapping rate to exons

Mapping rates as high as 80% for different RNA inputs

Full-Length BRB-seq exhibits reads distribution across the transcript’s entire length

>50'000 isoforms detected at 15M sequencing depth

Uniform detection of 22'000+ genes at 12 million reads per sample across 48 samples

Full-Length BRB-seq shows high demultiplexing rate and high mapping rate to exons

Mapping rates as high as 80% for different RNA inputs

Full-Length BRB-seq exhibits reads distribution across the transcript’s entire length

>50'000 isoforms detected at 15M sequencing depth

FAQs

See our products

Speak with our RNA sequencing experts

Subscribe to our newsletter

Receive email updates on our latest products and services, news and event updates and more. No ads, no spam.

Optional: Alithea performs Differential Gene Expression analysis