Full-length mRNA-seq combined with massive sample multiplexing.
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Our MERCURIUS™ Full-Length BRB-seq service is a convenient and scalable solution for projects of any size, enabling transcriptome-wide detection of full-length coding transcripts.
As part of the Full-Length BRB-seq service, users simply deliver their 96-well plates containing frozen purified RNA to us in Switzerland or the United States.
Upon receipt of the plates, our team prepares the libraries, then sequences to your desired read depth per sample, and then performs data pre-processing.
We return results, including raw fastq files, sequencing and alignment reports, and gene count matrices suitable for downstream differential expression analyses, once the data meet our rigorous quality control criteria.
During the process, we always keep clients informed at defined checkpoints so we can decide together how best to proceed to the next steps.
(Nanodrop and Fragment analyzer)
1 week
2 days
(Qubit, Fragment analyzer, shallow sequencing)
1 week
1 week
Raw FASTQ files, sample report file, QC files, and gene count tables
Number of genes detected per sample across a 48-sample MERCURIUS™ Full-Length BRB-seq run, stratified by expression level. All 48 samples show highly uniform gene detection, with approximately 22,000–24,000 genes detected at 12M reads/sample. The consistency across samples demonstrates the reproducibility of MERCURIUS™ Full-Length BRB-seq for high-throughput transcriptomic profiling.
Sequencing quality metrics across three data preprocessing stages for a MERCURIUS™ Full-Length BRB-seq library. (Left) 99.1% of reads were successfully demultiplexed, confirming highly accurate barcode assignment with minimal read loss. (Centre) 79.7% of mapped reads aligned to exonic regions, indicating efficient transcript capture. (Right) 82% of reads represent unique UMI-tagged molecules, indicating low duplication rates and high library complexity. Together, these metrics demonstrate the technical robustness and sequencing efficiency of MERCURIUS™ Full-Length BRB-seq.
Normalized read depth across gene bodies (5′ to 3′). MERCURIUS™ Full-Length BRB-seq produces a uniform coverage profile across the entire gene body length, closely matching Competitor N. In contrast, standard BRB-seq shows characteristic 3′-end enrichment, owing to its 3′-capture design. This illustrates the advantage of MERCURIUS™ Full-Length BRB-seq for applications requiring uniform transcript coverage, such as isoform detection, splice variant analysis, and allele-specific expression.
Number of detected transcripts as a function of downsampled sequencing depth for different technologies. MERCURIUS™ Full-Length BRB-seq closely tracks Competitor N across all read depths. Standard BRB-seq detects fewer transcripts, reflecting its 3′-capture design, which limits isoform resolution. These results demonstrate that MERCURIUS™ Full Length BRB-seq delivers competitor-equivalent transcript detection at higher throughput and lower cost.
Full-Length (Plant) BRB-seq performance shows 99% demultiplexing rate from raw data, 78% mapping rate, and 18% duplication rate of the 48 pooled samples and sequenced at 12 million reads per sample.
The gene body coverage shows a consistent and uniform reads distribution across the entire gene body for the Full-Length (Plant) BRB-seq protocol, comparable to the NEB protocol, while the BRB-seq protocol shows a significant 3′ bias due to its poly-A selection methodology.
To have access to the deep-sequenced dataset (9.5 M reads per sample) contact us.
To generate high-quality sequencing data we recommend starting with 10 – 1000 ng of total purified RNA per sample in 20μl.
The total RNA amount per pool should be at least 1000 ng.
The minimum recommended RIN number is 6 and the A260/230 ratio (Nanodrop) should be in the 1.5-2.2 range.
In addition to total RNA amount, it is important that the samples contain RNA of high integrity (RIN > 7) and are devoid of contaminants (Nanodrop A260/A230 between 1.8 and 2.2).
Full-Length BRB-seq provides comprehensive coverage of full-length mRNA transcripts for the detection of isoforms, alternative splicing, and differential promoter usage.
We therefore normally recommend sequencing 12-20 million reads per sample, enabling the reliable and unbiased detection of over 20,000 genes.
As part of our standard service pipeline, we align the generated data to the genome of choice, provide a detailed report on the alignment and gene counting statistics and, finally, provide ready-to-use gene count matrices for downstream analysis.
You can either book a call with our experts to discuss your project or submit your experimental details via our contact form so we can review your design and requirements.
Based on the goals, sample types, and scale of your study, we may recommend starting with a pilot project to optimise conditions and de-risk a larger screen.
If you’re interested in implementing the technology in your own lab instead, you can explore our MERCURIUS™ Full-Length BRB-seq kits on the dedicated kits page.
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