MERCURIUS™ Total Blood BRB-seq is a whole blood RNA-seq kit for purified human, rat, or mouse blood RNA samples, combining early multiplexing, inline globin depletion, and full-length total RNA sequencing in one streamlined workflow. The kit generates sequencing-ready libraries for coding and non-coding RNA analysis, while supporting applications such as transcript variant detection, alternative splicing analysis, and fusion gene discovery.
Available in 96- and 384-prep formats, the workflow enables up to 96 samples to be multiplexed in a single tube and is compatible with Illumina® and AVITI™ sequencing platforms.
Whole blood RNA-seq is powerful for population-scale transcriptomics, disease research, precision medicine, and biomarker discovery, but blood RNA contains highly abundant globin transcripts that can consume sequencing reads and reduce coverage of biologically informative genes.
MERCURIUS™ Total Blood BRB-seq integrates globin depletion directly into the library preparation workflow, helping increase usable reads for full-length blood transcriptome profiling.
Main applications
Distribution of the number of detected genes across 96 samples prepared with the MERCURIUS™ Blood BRB-seq library preparation kit and the MERCURIUS™ Total Blood BRB-seq. The libraries were sequenced at the same sequencing depth.
(Top) The distribution of reads mapped to non-globin and globin genes per sample for one 96 sample Total Blood BRB-seq library without globin depletion. An average of approximately one-third of reads mapped to globin genes. (Bottom) The distribution of reads mapped to non-globin and globin genes per sample for the same 96 samples in one Total Blood BRB-seq library treated with globin depletion. The reads associated with globin genes are drastically reduced, with more reads mapped to non-globin genes.
Number of detected genes at different downsampled sequencing depths for Total Blood BRB-seq and Blood BRB-seq. Total Blood BRB-seq consistently detects more genes across the full depth range, reaching approximately 20,000 genes at 6 million reads compared to ~19,000 for Blood BRB-seq.
Normalized read coverage across gene transcript bodies for Total Blood BRB-seq and Blood BRB-seq. Total Blood BRB-seq produces a broadly uniform coverage profile across the full transcript length, with coverage peaking early and remaining consistently high through to the 80th gene percentile. Blood BRB-seq displays a pronounced 3′-end bias, owing to its 3′-capture design.
Each Total Blood BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.
To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-sample kit can be used to prepare up to 96 samples distributed across up-to four different libraries.
Yes, the Total Blood BRB-seq workflow is validated on human, rat, and mouse blood. The rRNA depletion system is designed against a large panel of mammalian species and could work on other species. Feel free to contact us for more information.
The recommended range of RNA amount for each sample is of 10ng-100ng.
The minimum recommended RIN number is 7 and the A260/280 ratio (Nanodrop) should be A260/280 >1.5
The only difference betweenTotal Blood BRB-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the barcode sequence.
For a thorough description of Total Blood BRB-seq data processing, please refer to the kit user guide.
The recommended sequencing depth for the Total Blood BRB-seq libraries is between 2-10 M reads per sample, which is typically enough to detect the vast majority of expressed genes.
Go to the “Resources” section of this page and click on the button to download the barcode files for the product you are using.
Yes. If you are not ready to run kits internally, Alithea can run the workflow and deliver FASTQ files, gene count matrices, and analysis report files.
Whole blood RNA-seq refers to a specialized RNA sequencing method applied directly to blood samples. The main difference lies in sample preparation: whole blood RNA-seq requires extra depletion steps (e.g., globin reduction) to prevent highly abundant, non-informative transcripts from masking the rest of the data. The MERCURIUS™ Total Blood BRB-seq kit combines inline globin depletion with early multiplexing and full-length total RNA sequencing, making it one of the most scalable kits on the market.
Product
Catalog Number
MERCURIUS™ UDI X-Leap Expansion module
MERCURIUS™ Full-Length Post-Pooling Preparation Module (4 libraries)
Each kit includes 4 UDI pairs. Add the expansion module if you need more unique indexes (total 16 UDI pairs available).
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