Discover Sample Datasets
PBMCs, HEK293
Full-length single-cell RNA-seq is useful when gene-level expression alone is not enough. Compared with 3′ or 5′ counting approaches, full-length methods provide broader transcript coverage, helping researchers study isoform diversity, alternative splicing, gene-body coverage, expressed variants, fusion transcripts, and low-abundance genes in individual cells.
MERCURIUS™ Single-Cell FLASH-seq brings this full-length readout into an automation-friendly 96- or 384-well plate-based workflow for sorted cells.
FLASH-seq detects a median of approximately 11,000 genes per sample in HEK293T cells, outperforming Smart-seq3 and Smart-seq2 after downsampling all to 500k reads/sample.
Number of genes detected in human PBMCs, processed with different protocols, and the number of reads downsampled to 125,000 raw reads.
Gene body coverage shows a uniform read distribution across the entire gene body for the FLASH-seq protocol.
To have access to the deep-sequenced dataset (9.5M reads per sample) contact us.
We do not recommend very large cells, such as cardiomyocytes, or any other cell type incompatible with the FACS sorting step.
FLASH-seq is a plate-based full-length single-cell RNA-seq method designed to improve sensitivity, consistency, and workflow efficiency compared with earlier full-length scRNA-seq methods.
We have significantly enhanced the original FLASH-seq method to offer a streamlined workflow and superior data output. This plate-based technology (available in 96- and 384-well formats) features a novel, non-toxic tagmentation buffer and delivers ultra-sensitive gene detection, capturing up to two times more genes compared to other commercially available solutions.
We do not recommend very large cells, such as the cardiomyocytes, as they are not compatible with the FACS sorting step.
For optimal results, we require the cells to be directly FACS-sorted in the dedicated well plates. The 96- and 384-plates contain the lysis buffer. It is important for the user to sort the cells in the middle of the well. Please refer to the User Guide and the Sample Submission Guidelines for more details.
The average recommended sequencing depth is 250’000 reads/cell.
A full-length single-cell RNA-seq kit prepares sequencing libraries from individual cells while preserving transcript coverage across the gene body, enabling analysis beyond 3′ or 5′ gene counting.
Choose FLASH-seq when your study requires high sensitivity, sorted-cell control, rare cell analysis, isoform information, alternative splicing analysis, or full-length transcript coverage. Droplet-based methods are often better when the main goal is to profile very large numbers of cells.
Product
Number of Samples
MERCURIUS™ Cell lysis module
MERCURIUS™ Cell lysis module
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