MERCURIUS™

DRUG-seq service

DRUG-seq boosts screening efforts based on gene expression profiling by offering a unique RNA-seq service that is highly sensitive, extraction-free, massively multiplexed and extremely cost-effective.

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  • Services
  • Cell and Organoids (Extraction-free)
  • DRUG-seq service

Benefits

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Bulk RNA sequencing at scale
Unparalleled cost and throughput scalability in 96- and 384-well plates.
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Ideal for large projects

The more samples are processed together, the lower the cost per sample.
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Fast turnaround times

From samples to data in as little as two weeks.
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No prior RNA extraction needed

From frozen cell pellets directly into library preparation.
MERCURIUSTM

DRUG-seq service

The MERCURIUS™ DRUG-seq service offers the most comprehensive, high-throughput and cost-effective solution for gene expression analysis projects starting from 2D cell cultures and organoids.

As part of the DRUG-seq service, clients simply need to deliver to our service centers (located in Switzerland and the United States) 96- or 384-well plates with frozen cells.
 
Our DRUG-seq team will then process the plates the same week and return results in as little as two weeks.

By leveraging on the advantages the DRUG-seq system, such as cost-effectiveness and high-throughput, we are able to offer the most competitive price and turnaround time on the market.

As a result of our service, our clients receive raw sequencing data (fastq files), gene count matrices and analysis reports files.

Optional downstream differential gene expression analysis are also available upon request.
DRUG-seq sequencing in action

How it works

Step 1

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Client washes and freezes the cells

DRUG-seq-Sample-Submission-Guidelines

Step 2

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Client ships the plate(s) to Alithea

Sample submission form for MERCURIUSTM  DRUG-seq service

Step 3

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MERCURIUS™
DRUG-seq

 

time 2 days

 

Step 4

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Library QC - Client Checkpoint

(Qubit, Fragment analyzer, shallow sequencing)

time 1 week

 

Step 5

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Deep sequencing

 

 

 

time 1 week

Step 6

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Data analysis and reporting - Client Checkpoint

Example of sample report

time 1 week

Step 7

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Data

Optional: Alithea performs Differential Gene Expression analysis​

Decoding drug responses

MERCURIUS™ DRUG-seq empowers scalable transcriptome profiling by enabling precise gene detection, characterization of drug-response signatures, identification of synergistic/antagonistic drug effects, detailed pathway-level insights and much more.
AXXAM

Gene detection overview

Gene detection overview of a case study on 22'000 samples and 51 compounds in four doses, on 6 cell lines, sequenced at 1M reads per sample. 

Enables accurate clustering and co-clustering analysis based on unbiased gene expression measurement

Analysis of top DE genes
synergistic antagonistic effect -1

Detect potential synergistic/antagonistic effects of drug combinations

Enables detailed pathway analysis of biological processes

detailed pathway analysis-1

Sample datasets

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Hap1

Number of samples:
24

Reads per sample in demo dataset:
10'000 reads

To have access to the deep-sequenced dataset (7.3 M reads per sample) contact us.

Demo dataset file size:
13.3 MB

Download
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HepG2

Number of samples:
65

Reads per sample in demo dataset:
10'000 reads 

To have access to the deep-sequenced dataset (3.9 M reads per sample) contact us.

Demo dataset file size:
4.76 MB

Download
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HeLa

Number of samples:
24

Reads per sample in demo dataset:
10'000 reads

To have access to the deep-sequenced dataset (7.1 M reads per sample) contact us.

Demo dataset file size:
13.6 MB

Download

FAQs

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Relevant publications

Explore the latest, relevant publications in the industry to learn more about DRUG-seq.

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Want to do DRUG-seq in-house?

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Speak with one of our RNA experts

Book a one-on-one call with one of our RNA experts to discover how we can assist your next project.