DRUG-seq service

To guarantee high quality data, we normally request that each sample contains 15k-50k cells/well for 96 well-plate or 2k-10k cells/well for 384 well-plate. The minimum number of cells per pool should be 80k.

DRUG-seq is 3’-end RNA sequencing method and, as such, requires significantly less sequencing as compared to standard full-length RNA-seq in order to reach accurate gene quantification. We therefore normally recommend to sequence 1 to 10 million reads for each sample, which enables the reliable and unbiased detection of over 18’000 genes.

As part of our standard service pipeline, we align the generated data to the genome of choice, provide a detailed report on the alignment and gene counting statistics and, finally, provide ready-to-use gene count matrices for downstream analysis.