DRUG-seq | High-Throughput RNA-seq service

Benefits

Bulk RNA sequencing at scale: Unparalleled cost and throughput scalability in 96- and 384-well plates.

Ideal for large projects: The more samples are processed together, the lower the cost per sample.

Fast turnaround times: From samples to data in as little as two weeks.

No prior RNA extraction needed: From frozen cell pellets directly into library preparation.

MERCURIUS^TM

DRUG-seq service

The MERCURIUS™ DRUG-seq service offers the most comprehensive, high-throughput and cost-effective solution for gene expression analysis projects starting from 2D cell cultures and organoids.

As part of the DRUG-seq service, clients simply need to deliver to our service centers (located in Switzerland and the United states) 96- or 384-well plates with frozen cells.

Our DRUG-seq team will then process the plates the same week and return results in as little as two weeks.

By leveraging on the advantages the DRUG-seq system, such as cost-effectiveness and high-throughput, we are able to offer the most competitive price and turnaround time on the market.

As a result of our service, our clients receive raw sequencing data (fastq files), gene count matrices and analysis reports files.

Optional downstream differential gene expression analysis are also available upon request.

How it works

Step 1

Client washes and freezes the cells

Step 2

Client ships the plate(s) to Alithea

Sample submission form for MERCURIUS^TM DRUG-seq service

Step 3

MERCURIUS™ DRUG-seq

2 days

Step 4

Library QC - Client Checkpoint

(Qubit, Fragment analyzer, shallow sequencing)

1 week

Step 5

Deep sequencing

1 week

Step 6

Data analysis and reporting - Client Checkpoint

Example of sample report

1 week

Step 7

Data

Optional: Alithea performs Differential Gene Expression analysis

Sample datasets

Hap1

Number of samples:
24

Reads per sample in demo dataset:
10'000 reads

To have access to the deep-sequenced dataset (7.3 M reads per sample) contact us.

Demo dataset file size:
13.3 MB

Download

HepG2

Number of samples:
65

Reads per sample in demo dataset:
10'000 reads

To have access to the deep-sequenced dataset (3.9 M reads per sample) contact us.

Demo dataset file size:
4.76 MB

Download

HeLa

Number of samples:
24

Reads per sample in demo dataset:
10'000 reads

To have access to the deep-sequenced dataset (7.1 M reads per sample) contact us.

Demo dataset file size:
13.6 MB

Download

Validated cell lines

Cell line	Specie	Tissue	Culture type
A375	h.sapiens	Skin, malignant melanoma	adherent
GM12878	h.sapiens	PBMC, lymphoblastoid	suspension
H295R	h.sapiens	Adrenal gland, carcinoma	adherent
HAP1	h.sapiens	KBM-7 derived, chronic myelogenous leukemia	adherent
hASC	h.sapiens	Patient-derived adipose stromal cells	adherent
hASC-Adipocytes	h.sapiens	Differentiated hASC	adherent
HEK293	h.sapiens	Kidney embryonic	adherent
HeLa	h.sapiens	Cervix, adenocarcinoma	adherent
HepG2	h.sapiens	Liver, carcinoma	adherent
Huh7	h.sapiens	Liver, carcinoma	adherent
iNeurons	h.sapiens	Differentiated iPSC	adherent
MCF-7	h.sapiens	Breast, adenocarcinoma	adherent
PANC-1	h.sapiens	Pancreas, carcinoma	adherent
U2OS	h.sapiens	Bone, osteosarcoma	adherent

FAQs

Relevant publications

Explore the latest, relevant publications in the industry to learn more about DRUG-seq.

Explore

Want to do DRUG-seq in-house?

Experience reliable, cost-effective, and scalable products that deliver high-quality data for large projects.

complementary kit

Cells and organoids (Extraction-free)

Purified RNA

Accessories

Cells and Organoids (EXTRACTION-FREE)

PURIFIED RNA

Whole Blood RNA

Cells and organoids (Extraction-free)

Purified RNA

Accessories

Cells and Organoids (EXTRACTION-FREE)

PURIFIED RNA

Whole Blood RNA

DRUG-seq service

Benefits

DRUG-seq service

How it works

Client washes and freezes the cells

Client ships the plate(s) to Alithea

MERCURIUS™ DRUG-seq

Library QC - Client Checkpoint

Deep sequencing

Data analysis and reporting - Client Checkpoint

Data

Optional: Alithea performs Differential Gene Expression analysis

Sample datasets

Hap1

HepG2

HeLa

Validated cell lines

FAQs

Relevant publications

Want to do DRUG-seq in-house?

Speak with one of our RNA experts

Get in touch

DRUG-seq service

Benefits

DRUG-seq service

How it works

Client washes and freezes the cells

Client ships the plate(s) to Alithea

MERCURIUS™ DRUG-seq

Library QC - Client Checkpoint

Deep sequencing

Data analysis and reporting - Client Checkpoint

Data

Optional: Alithea performs Differential Gene Expression analysis​

Sample datasets

Hap1

HepG2

HeLa

Validated cell lines

FAQs

Relevant publications

Want to do DRUG-seq in-house?

Speak with one of our RNA experts

Optional: Alithea performs Differential Gene Expression analysis