MERCURIUS™

Spheroid DRUG-seq service

Spheroid DRUG-seq boosts screening efforts based on gene expression profiling of spheroids and organoids by offering a unique RNA-seq service that is highly sensitive, extraction-free, massively multiplexed and extremely cost-effective.

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Benefits

Bulk RNA sequencing at scale: Unparalleled cost and throughput scalability in 96- and 384-well plates.

No prior RNA extraction needed: From frozen organoids directly into library preparation.

Ideal for large projects: The more samples are processed together, the lower the cost per sample.

Fast turnaround times: From samples to data in as little as two weeks.

MERCURIUS^TM

Spheroid DRUG-seq service

The MERCURIUS™ Spheroid DRUG-seq service offers the most comprehensive, high-throughput and cost-efficient solution for gene expression analysis projects starting from 3D organoids or spheroids.

As part of the Spheroid DRUG-seq service, clients simply need to deliver 96- or 384-well plates with frozen organoids to our service centers (located in Switzerland and the United States).

Our Spheroid DRUG-seq team will then process the plates the same week and return results in as little as two weeks.

By leveraging on the advantages the DRUG-seq system, such as cost-effectiveness and high-throughput, we are able to offer the most competitive price and turnaround time on the market.

As a result of our service, our clients receive raw sequencing data (fastq files), gene count matrices and analysis reports files.

Optional downstream differential gene expression analysis is also available upon request.

How it works

Step 1

Client prepares the samples

Sample preparation guidelines for MERCURIUSTM Organoid DRUG-seq service

Step 2

Client ships samples to Alithea

Sample submission form for MERCURIUSTM Organoid DRUG-seq service

Step 3

MERCURIUS™ Spheroid DRUG-seq

2 days

Step 4

Library QC - Client Checkpoint

(Qubit, Fragment analyzer, shallow sequencing)

time 1 week

Step 5

Deep sequencing

1 week

Step 6

Data analysis and reporting - Client Checkpoint

time 1 week

Step 7

Data

Optional: Alithea performs Differential Gene Expression analysis

Sample datasets

Prostate cancer

Number of samples:
12

Reads per sample in demo dataset:
10'000 reads

To have access to the deep-sequenced dataset (24.5M reads per sample) contact us.

Demo dataset file size:
7.16 MB

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FAQs

Spheroid DRUG-seq is 3’-end RNA sequencing method and, as such, requires significantly less sequencing as compared to standard full-length RNA-seq in order to reach accurate gene quantification. We therefore normally recommend sequencing between 1 to 10 million reads for each sample, which enables the reliable and unbiased detection of over 18’000 genes.

Relevant publications

Explore the latest, relevant publications in the industry to learn more about our technologies.

Explore

Want to do Spheroid DRUG-seq in-house?

Our DRUG-seq kits make it possible to process virtually any number of RNA samples in one single tube, which helps reduce technical variation, turnaround times and costs.

Experience sample to sequencing-ready library in one day.

COMPLEMENTARY KIT

Spheroid DRUG-seq service

Benefits

Spheroid DRUG-seq service

How it works

Client prepares the samples

Client ships samples to Alithea

MERCURIUS™ Spheroid DRUG-seq

Library QC - Client Checkpoint

Deep sequencing

Data analysis and reporting - Client Checkpoint

Data

Optional: Alithea performs Differential Gene Expression analysis​

Sample datasets

Prostate cancer

FAQs

Relevant publications

Want to do Spheroid DRUG-seq in-house?

Speak with one of our RNA experts

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Optional: Alithea performs Differential Gene Expression analysis