Bulk RNA sequencing at scale
Massively multiplexed RNA-seq offers unparalleled cost and throughput scalability.
Ideal for projects of all sizes
You can trust us. We have ran projects from as low as 6 samples to as many as 9,000.
Fast turnaround times
From samples to data in as fast as 1.5 months.
What we do
Organoid DRUG-seq service
The MERCURIUS™ Organoid DRUG-seq service offers the most comprehensive, high-throughput and cost-efficient solution for gene expression analysis projects starting from 3D organoids or spheroids.
As part of the Organoid DRUG-seq service, we send our clients a proprietary Lysis Buffer, optimized for both organoid and spheroid lysis and downstream enzymatic reactions, without having to perform RNA isolation in between.
After mixing the Lysis Buffer with their samples, the client sends us the organoid/spheroid lysates, which are then ready to be processed in the DRUG-seq workflow.
By leveraging the advantages of the Organoid DRUG-seq system, such as cost-efficiency and high throughput, we can offer the most competitive price and turnaround time on the market.
As a result of our service, our clients receive raw sequencing data (fastq files), gene count matrices, and analysis reports files.
Optional downstream differential gene expression analysis is also available upon request.
How does it work
Alithea ships the Organoid Lysis Buffer
Incoming QC - Client Checkpoint(Nanodrop, Ribogreen, Tapestation)
MERCURIUS™ Organoid DRUG-seq for Illumina®
Library QC - Client Checkpoint(Qubit, Fragment analyzer, shallow sequencing)
Deep sequencing - Illumina
Data analysis and reporting - Client Checkpoint
Optional: Alithea performs Differential Gene Expression analysis
To guarantee high quality data, we normally request that each sample contains at least 200ng of total RNA in at least 10μl.
In addition to total RNA amount, it is important that the samples contain RNA of high integrity (RIN > 6) and are devoid of contaminants (Nanodrop A260/A230 between 1.8 and 2.2).
BRB-seq is 3’-end RNA sequencing method and, as such, requires significantly less sequencing as compared to standard full-length RNA-seq in order to reach accurate gene quantification. We therefore normally recommend to sequence 4 to 5 million reads for each sample, which enables the reliable and unbiased detection of over 18’000 genes.
As part of our standard service pipeline, we align the generated data to the genome of choice, provide a detailed report on the alignment and gene counting statistics and, finally, provide ready-to-use gene count matrices for downstream analysis.
Speak with our RNA sequencing experts
Need guidance or have questions about BRB-seq and our service?
Easily book a call with our BRB-seq experts.