MERCURIUS™

Low-input FLASH-seq kits

Ultra-sensitive and full-length RNA-seq technology for low-input RNA samples


Catalog number	# 10931
Number of samples The total number of samples that can be lysed with the provided volume.	96
Total preps and plate format How many library preps can be prepared in total with one kit. One plate per kit.	96
Documentation	User guide

96 Cat ## 10931
Total reactions How many library preps can be prepared in total with one kit	96
Download Manual

Benefits

The MERCURIUS™ Extraction-free Low-input FLASH-seq kits contain all the oligos and enzymes needed to go from low-input RNA samples to sequencing-ready libraries.

Ultra-sensitive: Up to 2x more genes detected than other commercially available solutions. Ideal for rare cell capture.

Dedicated to low-input samples: Low RNA inputs from precious samples (1 pg to 1 ng per well).

Full-length transcript coverage: From differential gene expression to splicing variants and isoform detection.

No need for prior RNA extraction: Simply FACS-sort the cells in the wells containing the buffer for complete lysis and efficient reverse transcription.

One-day lab workflow: Convenient and short protocol from samples to sequencing-ready libraries in only 7H30.

Suited to automation: Enables seamless integration into high-throughput workflows, furter reducing hands-on time and increasing scalability, reproducibility and efficiency.

Experimental workflow at a glance
Performance
Product specifications
Cells compatibility

Low-input FLASH-seq-1

1. MERCURIUS™ FLASH-Seq Shows The Highest Sensitivity In Gene Detection

FLASH_seq-highest sensitivity in gene detection

The number of detected genes in HEK 293T cells processed with different protocols. Reads were downsampled to 500,000 raw reads.

2. The Sensitivity Of MERCURIUS™ FLASH-Seq Is Retained Even In Highly Heterogeneous Populations

The MERCURIUSTM FLASH-seq sensitivity is retained even in highly heterogeneous populations

Number of genes detected in human PBMCs, processed with different protocols, and the number of reads downsampled to 125,000 raw reads.

3. MERCURIUS™ FLASH-Seq Is A Full-Length ScRNA-Seq Protocol

Gene body coverage shows a uniform read distribution across the entire gene body for the FLASH-seq protocol.

4. MERCURIUS™ FLASH-Seq Shows High Sensitivity For Low Sample Inputs

FLASH-seq shows high sensitivity for low sample inputs

The number of genes detected in HEK 293T cells using different RNA inputs (from 2.5 pg to 250 pg) at different sequencing depths.

5. MERCURIUS™ FLASH-Seq Can Discriminate Rare Cell Populations In Heterogeneous Samples

UMAP of hPBMC, automatically annotated using Azimuth (Hao et al., 2021).

For (application)	full-length mRNA sequencing for single-cell
For use with (equipment)	Illumina, AVITI, Ultima Gneomics and MGI instruments
Species compatibility	All eukaryotic species
Available formats	96 preps
Shipping conditions	Dry ice
Storage conditions	-20C

We do not recommend very large cells, such as cardiomyocytes, or any other cell type incompatible with the FACS sorting step.

1. MERCURIUS™ FLASH-Seq Shows The Highest Sensitivity In Gene Detection

The number of detected genes in HEK 293T cells processed with different protocols. Reads were downsampled to 500,000 raw reads.

2. The Sensitivity Of MERCURIUS™ FLASH-Seq Is Retained Even In Highly Heterogeneous Populations

Number of genes detected in human PBMCs, processed with different protocols, and the number of reads downsampled to 125,000 raw reads.

3. MERCURIUS™ FLASH-Seq Is A Full-Length ScRNA-Seq Protocol 

Gene body coverage shows a uniform read distribution across the entire gene body for the FLASH-seq protocol.

4. MERCURIUS™ FLASH-Seq Shows High Sensitivity For Low Sample Inputs 

The number of genes detected in HEK 293T cells using different RNA inputs (from 2.5 pg to 250 pg) at different sequencing depths.

5. MERCURIUS™ FLASH-Seq Can Discriminate Rare Cell Populations In Heterogeneous Samples

UMAP of hPBMC, automatically annotated using Azimuth (Hao et al., 2021).

Product specifications

Sample dataset

PBMCs

Number of samples:
96

Reads per sample in demo dataset:

10'000 reads

To have access to the deep-sequenced dataset (9.5M reads per sample) contact us.

Demo dataset file size:

79.9 MB

Download

HEK293

Number of samples:
191

Reads per sample in demo dataset:

250'000 reads

Demo dataset file size:

4.9 GiB

FAQs

MERCURIUS™ FLASH-seq is a plate-based method that focuses on polyadenylated RNA, which includes most mRNAs. It provides detailed information on gene structure and alternative splicing. Its ability to capture full-length mRNA transcripts and detect low-abundance genes makes it a powerful tool for gene expression profiling.

FLASH-seq protocol was designed to enhance both the sensitivity and efficiency of single-cell mRNA sequencing compared to the most famous plate-based method, Smart-seq2

Read more about the technology in our blog posts: here and here.
We have significantly enhanced the original FLASH-seq method to offer a streamlined workflow and superior data output. This plate-based technology (available in 96- and 384-well formats) features a novel, non-toxic tagmentation buffer and delivers ultra-sensitive gene detection, capturing up to two times more genes compared to other commercially available solutions.
Immunology

FLASH-seq is ideal for studying rare immune cell populations, such as antigen-specific T cells or exhausted T cell subsets in chronic infections and cancer. Its high sensitivity allows researchers to capture subtle transcriptomic differences, splicing events, and even intracellular viral transcripts at the single-cell level.

Cancer
Dissect tumor heterogeneity by sequencing rare cancer stem cells or circulating tumor cells. FLASH-seq enables detection of expressed mutations, splicing isoforms, and gene fusions, providing deep insights into clonal evolution and therapeutic resistance in individual cancer cells.

Neurobiology
In complex tissues like the brain, FLASH-seq empowers researchers to analyze rare neuronal subtypes or glial cells with high resolution. It reveals alternative splicing events and single-nucleotide variants that may be critical in neurodevelopmental or neurodegenerative disorders.

Genetic Therapies & ASOs
FLASH-seq can be used to assess the impact of antisense oligonucleotides (ASOs) on target splicing at single-cell resolution. This enables precise tracking of treatment efficacy across rare or responsive cell subsets within a population.

Intracellular Pathogens
Detect intracellular viral RNA in infected cells—even when those cells are extremely rare. FLASH-seq allows you to simultaneously monitor host responses and viral transcript presence, crucial for studying latent infections or early-stage viral spread.
We require the samples to be added to the lysis buffer that we will ship. The first step is to distribute the buffer in the wells of the dedicated plate(s) that will provide as well. The second step is to add the samples, in the lysis buffer and snap freeze the plates.

Please note! To preserve sample quality during shipping, plates must be placed between dry ice—not just on top. Inadequate cooling may compromise RNA integrity!

Relevant publications

Explore the latest, relevant publications in the industry to learn more about our technologies.

Explore

Not ready to use our kits?

Let us do it for you. Our team will deliver the raw sequencing data (fastq files), gene count matrices and analysis report files. A cost-efficient option suitable for projects of all sizes.

DISCOVER MORE

Low-input FLASH-seq kits

Benefits

1. MERCURIUS™ FLASH-Seq Shows The Highest Sensitivity In Gene Detection​

2. The Sensitivity Of MERCURIUS™ FLASH-Seq Is Retained Even In Highly Heterogeneous Populations​

3. MERCURIUS™ FLASH-Seq Is A Full-Length ScRNA-Seq Protocol ​

4. MERCURIUS™ FLASH-Seq Shows High Sensitivity For Low Sample Inputs ​