MERCURIUS™
Single-cell FLASH-seq kits
Ultra-sensitive, full-length and plate-based single cell RNA-seq technology for FACS-sorted cells.
Library preparation kits for Illumina®, AVITI™, Ultima Genomics and MGI.
Catalog number | #10921 |
#10923 |
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Number of samples
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96 | 384 | ||
Total preps and plate format
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96 | 384 | ||
Documentation |
Cat ##10921
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- Products
- Cells and Organoids (Extraction-free)
- DRUG-seq kits
Benefits
The MERCURIUS™ Extraction-free FLASH-seq kits contain all the oligos and enzymes needed to go from FACS-sorted cells to sequencing-ready libraries.
- Ultra-sensitive
- Up to 2x more genes detected than other commercially available solutions. Ideal for rare cell capture.
- Full-length transcript coverage
- From differential gene expression to splicing variants and isoform detection.
- No need for prior RNA extraction
- Simply FACS-sort the cells in the wells containing the buffer for complete lysis and efficient reverse transcription.
- One-day lab workflow
- Convenient and short protocol from samples to sequencing-ready libraries in only 7H30.
- Suited to automation
- Enables seamless integration into high-throughput workflows, furter reducing hands-on time and increasing scalability, reproducibility and efficiency.
- Experimental workflow at a glance
- Performance
- Product specifications
- Cells compatibility
1. MERCURIUS™ FLASH-Seq Shows The Highest Sensitivity In Gene Detection

The number of detected genes in HEK 293T cells processed with different protocols. Reads were downsampled to 500,000 raw reads.
2. The Sensitivity Of MERCURIUS™ FLASH-Seq Is Retained Even In Highly Heterogeneous Populations

Number of genes detected in human PBMCs, processed with different protocols, and the number of reads downsampled to 125,000 raw reads.
3. MERCURIUS™ FLASH-Seq Is A Full-Length ScRNA-Seq Protocol

Gene body coverage shows a uniform read distribution across the entire gene body for the FLASH-seq protocol.
4. MERCURIUS™ FLASH-Seq Shows High Sensitivity For Low Sample Inputs
The number of genes detected in HEK 293T cells using different RNA inputs (from 2.5 pg to 250 pg) at different sequencing depths.
5. MERCURIUS™ FLASH-Seq Can Discriminate Rare Cell Populations In Heterogeneous Samples

UMAP of hPBMC, automatically annotated using Azimuth (Hao et al., 2021).
For (application) |
full-length mRNA sequencing for single-cell |
For use with (equipment) |
Illumina, AVITI, Ultima Gneomics and MGI instruments |
Species compatibility |
All eukaryotic species |
Available formats |
96 and 384 preps |
Shipping conditions |
Dry ice |
Storage conditions |
-20C |
We do not recommend very large cells, such as cardiomyocytes, or any other cell type incompatible with the FACS sorting step.
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1. MERCURIUS™ FLASH-Seq Shows The Highest Sensitivity In Gene Detection
The number of detected genes in HEK 293T cells processed with different protocols. Reads were downsampled to 500,000 raw reads.
2. The Sensitivity Of MERCURIUS™ FLASH-Seq Is Retained Even In Highly Heterogeneous Populations
Number of genes detected in human PBMCs, processed with different protocols, and the number of reads downsampled to 125,000 raw reads.
3. MERCURIUS™ FLASH-Seq Is A Full-Length ScRNA-Seq Protocol
Gene body coverage shows a uniform read distribution across the entire gene body for the FLASH-seq protocol.
4. MERCURIUS™ FLASH-Seq Shows High Sensitivity For Low Sample Inputs
The number of genes detected in HEK 293T cells using different RNA inputs (from 2.5 pg to 250 pg) at different sequencing depths.
5. MERCURIUS™ FLASH-Seq Can Discriminate Rare Cell Populations In Heterogeneous Samples
UMAP of hPBMC, automatically annotated using Azimuth (Hao et al., 2021).
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For (application)
full-length mRNA sequencing for single-cell
For use with (equipment)
Illumina, AVITI, Ultima Gneomics and MGI instruments
Species compatibility
All eukaryotic species
Available formats
96 and 384 preps
Shipping conditions
Dry ice
Storage conditions
-20C
-
We do not recommend very large cells, such as cardiomyocytes, or any other cell type incompatible with the FACS sorting step.
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MERCURIUS™ FLASH-seq is a plate-based method that focuses on polyadenylated RNA, which includes most mRNAs. It provides detailed information on gene structure and alternative splicing. Its ability to capture full-length mRNA transcripts and detect low-abundance genes makes it a powerful tool for gene expression profiling.
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We have significantly enhanced the original FLASH-seq method to offer a streamlined workflow and superior data output. This plate-based technology (available in 96- and 384-well formats) features a novel, non-toxic tagmentation buffer and delivers ultra-sensitive gene detection, capturing up to two times more genes compared to other commercially available solutions.
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ImmunologyFLASH-seq is ideal for studying rare immune cell populations, such as antigen-specific T cells or exhausted T cell subsets in chronic infections and cancer. Its high sensitivity allows researchers to capture subtle transcriptomic differences, splicing events, and even intracellular viral transcripts at the single-cell level.Cancer
Dissect tumor heterogeneity by sequencing rare cancer stem cells or circulating tumor cells. FLASH-seq enables detection of expressed mutations, splicing isoforms, and gene fusions, providing deep insights into clonal evolution and therapeutic resistance in individual cancer cells.Neurobiology
In complex tissues like the brain, FLASH-seq empowers researchers to analyze rare neuronal subtypes or glial cells with high resolution. It reveals alternative splicing events and single-nucleotide variants that may be critical in neurodevelopmental or neurodegenerative disorders.Genetic Therapies & ASOs
FLASH-seq can be used to assess the impact of antisense oligonucleotides (ASOs) on target splicing at single-cell resolution. This enables precise tracking of treatment efficacy across rare or responsive cell subsets within a population.Intracellular Pathogens
Detect intracellular viral RNA in infected cells—even when those cells are extremely rare. FLASH-seq allows you to simultaneously monitor host responses and viral transcript presence, crucial for studying latent infections or early-stage viral spread. -
The MERCURIUS™ FLASH-seq protocol is fully compatible with whole FACS-sorted cells.
We do not recommend very large cells, such as the cardiomyocytes, as they are not compatible with the FACS sorting step.
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For optimal results, we require the cells to be directly FACS-sorted in the dedicated well plates. The 96- and 384-plates contain the lysis buffer. It is important for the user to sort the cells in the middle of the well. Please refer to the User Guide and the Sample Submission Guidelines for more details.
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The average recommended sequencing depth is 250'000 reads/cell.


Relevant publications
Explore the latest, relevant publications in the industry to learn more about our technologies.
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