MERCURIUS™

BRB-seq
Library preparation kits for Illumina®

24, 96, 384 RNA-seq library preps in one tube.

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CP_AG_FrontTall_Comp_Rounded_BRB_Req
Cat #10812 CP_AG_FrontTall_Comp_Transparent_Small
Total reactions How many library preps can be prepared in total with one kit 24
RNA multiplexing format How many library preps can be prepared in total with one kit 24
UDI pairs included How many library preps can be prepared in total with one kit 1
icn_blood-brb-seq
Cat #10813 CP_AG_FrontTall_Comp_Transparent_Small
Total reactions How many library preps can be prepared in total with one kit 96
RNA multiplexing format How many library preps can be prepared in total with one kit 96
UDI pairs included How many library preps can be prepared in total with one kit 4
icn_blood-brb-seq
Cat #11013 CP_AG_FrontTall_Comp__Small
Total reactions How many library preps can be prepared in total with one kit 384
RNA multiplexing format How many library preps can be prepared in total with one kit 96
UDI pairs included How many library preps can be prepared in total with one kit 4
icn_blood-brb-seq
Cat #10814 CP_AG_FrontTall_Comp_Transparent_Small
Total reactions How many library preps can be prepared in total with one kit 384
RNA multiplexing format How many library preps can be prepared in total with one kit 384
UDI pairs included How many library preps can be prepared in total with one kit 4
icn_blood-brb-seq
Cat #11014 CP_AG_FrontTall_Comp__Small
Total reactions How many library preps can be prepared in total with one kit 1536
RNA multiplexing format How many library preps can be prepared in total with one kit 384
UDI pairs included How many library preps can be prepared in total with one kit 4
icn_blood-brb-seq

Kit Pricing

Discover the unique scalability offered by BRB-seq

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Custom Format

For more reactions, higher multiplexing and more UDIs

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Benefits

The MERCURIUS™ BRB-seq library preparation kits for Illumina® contain all the oligos and enzymes needed to go from purified RNA to sequencing-ready DNA libraries.

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Bulk RNA sequencing at scale

Perform up-to 384 RNA-seq library preparations in one single tube.

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Streamlined data pre-processing

Demultiplex and align your BRB-seq data with our easy-to-use cloud-based platform.

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BRB-seq as a flexible solution

For projects of all sizes:
from 24 to 1536 reactions in one kit.
All-inclusive.

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One-day lab workflow

Convenient and short protocol from samples to sequencing-ready libraries in one day.

Experimental workflow at a glance

Product Specifications

For use with (application)
3' mRNA sequencing
For use with (equipment)
Illumina NGS instruments
Validated species
Human, Mouse, Arabidopsis, Zebrafish, Drosophila
Species compatibility
All eukaryotic species
Available formats
24, 96, 384, 1536 reactions
Shipping conditions
Dry ice
Storage conditions
-20C

FAQs

  • Each BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.

    To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.

  • The recommended range of RNA amount for each sample is of 50ng-1μg, normally the more RNA, the better.

    The minimum recommended RIN number is 6 and the A260/230 ratio (Nanodrop) should be in the 1.5-2.2 range.

  • The only difference between BRB-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the BRB-seq barcode sequence.

    For a thorough description of BRB-seq data processing, please refer to the BRB-seq kit user guide. In order to facilate as much as possible BRB-seq data analysis, we have also created a dedicate pipeline which can be found in our “SOFTWARE” page.

  • One of the key advantages of BRB-seq is that it does not only save reagents and cost in the library preparation stage, but also in the sequencing one.

    As opposed to standard RNA-seq, where 20M-30M reads per sample are required, we normally recommend to sequence BRB-seq libraries at a depth of 4M-5M reads per sample, which is normally enough to detect the vast majority of expressed genes.

Try our services

Our BRB-seq service delivers raw sequencing data (fastq files), gene count matrices and analysis report files. A cost-efficient option suitable for projects of all sizes.

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Speak with our RNA sequencing experts

Book a one-on-one call with one of our RNA experts to discover how we can assist your next project.