MERCURIUS™ BRB-seq kit for Illumina®

	Cat #10813	Cat #11013	Cat #10814	Cat #11014	4x Cat #11014	16x Cat #11014
Total reactions How many library preps can be prepared in total with one kit	96	384	384	1'536	6'144	24'576
RNA and plate multiplexing format How many samples can be multiplexed in one single tube	96	96	384	384	384	384
Barcoded oligo-dT plates included	1	4	1	4	16	64
UDI pairs included How many individual libraries can be sequenced together	4	4	4	4	16	16
Documentation	Download user guide			Download barcode files

Cat #10813
Total reactions How many library preps can be prepared in total with one kit	96
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Cat #11013
Total reactions How many library preps can be prepared in total with one kit	384
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Cat #10814
Total reactions How many library preps can be prepared in total with one kit	384
RNA multiplexing format How many library preps can be prepared in total with one kit	384
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Cat #11014
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit	384
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

4x Cat #11014
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit	384
UDI pairs included How many library preps can be prepared in total with one kit	16
Download Manual

16x Cat #11014
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit	384
UDI pairs included How many library preps can be prepared in total with one kit	16
Download Manual

Bulk RNA sequencing at scale

Perform up-to 384 RNA-seq library preparations in one single tube.

BRB-seq as a flexible solution

For projects of all sizes:
from 96 to 24'576 reactions in one kit.
All-inclusive.

One-day lab workflow

Convenient and short protocol from samples to sequencing-ready libraries in one day.

Performance

Uniform detection of 12'000+ genes at 1 million reads per sample across 384 samples

Distribution of the number of detected genes across...

Comparison of library complexity between BRB-seq and TruSeq at a comparable sequencing depth

Detected genes (left) and differentially expressed...

Benchmarking the performance of BRB-seq for DE genes at 3M reads/sample with other commercial platforms

Number of detected genes for the non-differentiated...

Benchmarking the performance for library prep between BRB-seq and two other commercial kits

Genome-wide Pearson correlation of mapped reads for...

4000+ DE genes with 3M reads/sample at FDR 0.5

Number of differentially expressed genes across three...

Product Specifications

For (application)

3' mRNA sequencing

For use with (equipment)

Illumina NGS instruments

Species compatibility

All eukaryotic species

Available formats

96, 384, 1'536, 6'144 and 24'576 reactions

Shipping conditions

Dry ice

Storage conditions

-20C

Human

Number of samples: 96

Reads per sample in demo dataset:

10'000 reads

To have access to the deep-sequenced dataset (9.5M reads per sample) contact us.

Demo dataset file size:

79.9 MB

Mouse

Number of samples: 96

Reads per sample in demo dataset:

10'000 reads

To have access to the deep-sequenced dataset (8.9M reads per sample) contact us.

Demo dataset file size:

78.6 MB

Cattle

Number of samples: 54

Reads per sample in demo dataset:

10'000 reads

To have access to the deep-sequenced dataset (5.7M reads per sample) contact us.

Demo dataset file size:

28.4 MB

Wild boar

Number of samples: 54

Reads per sample in demo dataset:

10'000 reads

To have access to the deep-sequenced dataset (6.3M reads per sample) contact us.

Demo dataset file size:

27.7 MB

Bat

Number of samples: 23

Reads per sample in demo dataset:

10'000 reads

To have access to the deep-sequenced dataset (9M reads per sample), contact us.

Demo dataset file size:

23.3 MB
Arabidopsis

Number of samples: 96

Reads per sample in demo dataset:

10'000 reads

To have access to the deep-sequenced dataset (3M reads per sample), contact us.

Demo dataset file size:

47.9 MB

Spinach

Number of samples: 96

Reads per sample in demo dataset:

10'000 reads

To have access to the deep-sequenced dataset (9M reads per sample) contact us.

Demo dataset file size:

83.9 MB
Drosophila

Number of samples: 96

Reads per sample in demo dataset:

10'000 reads

To have access to the deep-sequenced dataset (3M reads per sample), contact us.

Demo dataset file size:

38.2 MB
Zebrafish

Number of samples: 64

Reads per sample in demo dataset:

10'000 reads

To have access to the deep-sequenced dataset (4M reads per sample) contact us.

Demo dataset file size:

31.9 MB

Rainbow trout

Number of samples: 16

Reads per sample in demo dataset:

10'000 reads

To have access to the deep-sequenced dataset (5.3M reads per sample) contact us.

Demo dataset file size:

8.76 MB

Medaka

Number of samples: 96

Reads per sample in demo dataset:

10'000 reads

To have access to the deep-sequenced dataset (4.7M reads per sample) contact us.

Demo dataset file size:

50.6 MB

Each BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.

To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
The recommended range of RNA amount for each sample is of 50ng-1μg, normally the more RNA, the better.

The minimum recommended RIN number is 6 and the A260/230 ratio (Nanodrop) should be in the 1.5-2.2 range.
The only difference between BRB-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the BRB-seq barcode sequence.

For a thorough description of BRB-seq data processing, please refer to the BRB-seq kit user guide.
One of the key advantages of BRB-seq is that it does not only save reagents and cost in the library preparation stage, but also in the sequencing one.

As opposed to standard RNA-seq, where 20M-30M reads per sample are required, we normally recommend to sequence BRB-seq libraries at a depth of 4M-5M reads per sample, which is normally enough to detect the vast majority of expressed genes.

MERCURIUS™

BRB-seq
Library preparation kits for Illumina^®

Kit Pricing

Custom Format

Benefits