MERCURIUS™ BRB-seq kit for Illumina® and AVITI™


Catalog number	#10813	#11013	#10814	#11014
Total preps How many library preps can be prepared in total with one kit	96	384	384	1'536
RNA and plate multiplexing format How many samples can be multiplexed in one single tube	96	96	384	384
Barcoded oligo-dT plates included	1	4	1	4
UDI pairs included How many individual libraries can be sequenced together	4	4	4	4
Documentation	User guide		Barcode files

Cat ##10813
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Cat ##11013
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Cat ##10814
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit	384
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Cat ##11014
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit	384
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Accessories

UDI expansion

module

Compatible with the MERCURIUS™ product line.
12 UDI pairs.
Flexible dual indexing solutions.

Find out more

Benefits

The MERCURIUS™ BRB-seq library preparation kits for Illumina® and AVITI contain all the oligos and enzymes needed to go from purified RNA to sequencing-ready libraries.


Bulk RNA sequencing at scale	BRB-seq as a flexible solution	One-day lab workflow
Perform up-to 384 RNA-seq library preparations in one single tube.	For projects of all sizes: from 96 to 24'576 reactions in one kit. All-inclusive.	Convenient and short protocol from samples to sequencing-ready libraries in one day.

Experimental workflow at a glance
Performance

1. Uniform detection of 12'000+ genes at 1 million reads per sample across 384 samples

Distribution of the number of detected genes across 384 samples prepared with the MERCURIUS™ BRB-seq library preparation kit. The library was sequenced at an average of 1 million reads

2. Comparison of library complexity between BRB-seq and TruSeq at a comparable sequencing depth

Detected genes (left) and differentially expressed genes (right) obtained on the same samples with BRB-seq and TruSeq. At comparable sequencing depth, the performance of BRB-seq data is comparable with TruSeq libraries.

3. Benchmarking the performance of BRB-seq for DE genes at 3M reads/sample with other commercial platforms

Number of detected genes for the non-differentiated (ND) and differentiated (D) Adipose Stromal Population Cells between BRB-seq and two other commercial kits. The expressed genes are split into three categories: Lowly Expressed (left, 0 < CPM < 10), Mid expressed (middle, 10 < CPM < 100), and Highly expressed (right, CPM > 100). To note: all replicates were prepared from the same RNA sample and the sequencing depth was 3M reads/sample.

4. Benchmarking the performance for library prep between BRB-seq and two other commercial kits

Genome-wide Pearson correlation of mapped reads for the non-differentiated (ND) and differentiated (D) Adipose Stromal Population Cells between BRB-seq and two other commercial kits. A high correlation was observed both within technical replicates and across different library prep kits.

5. 4000+ DE genes with 3M reads/sample at FDR 0.5

Number of differentially expressed genes across three commercial platforms, including BRB-seq, at different FDR cutoff values. To note: all replicates were prepared from the same RNA sample and the sequencing depth was 3M reads/sample.

Product specifications

For (application)	3' mRNA sequencing
For use with (equipment)	Illumina and AVITI NGS instruments
Species compatibility	All eukaryotic species
Available formats	96, 384, 1'536, 6'144 and 24'576 preps
Shipping conditions	Dry ice
Storage conditions	-20C

Citations
Please visit the dedicated page for a complete list.
- Sofa Pavlou, Stefanie Foskolou, Nikolaos Patikas, Sarah F. Field, Evangelia K. Papachristou, Clive D’ Santos, Abigail R. Edwards, Kamal Kishore, Rizwan Ansari, Sandeep S. Rajan, Hugo J. R. Fernandes, Emmanouil Metzakopian. 2023. CRISPR‑Cas9 genetic screen leads to the discovery of L‑Moses, a KAT2B inhibitor that attenuates Tunicamycin‑mediated neuronal cell death. Scientific Reports, 13:3934. https://doi.org/10.1038/s41598-023-31141-6
- Christian, W. T., Tiphaine, O.-M., Nava, E., Christian, H., Federico, A. S., Jonathan, N., Dominique, C., Cécile, G., Laurence, M., Sophia, S. W., Shehnaz, K. H., Ioannis, T., Matthias, C., Andri, R., Manuel, B., Matthias, H., Patrick, S., Enos, B., Huldrych, F. G., Pejman, M., Paul, J. M., Charles, S. R., Caroline, B., & Jacques, F. 2020. Genetic variation near CXCL12 is associated with susceptibility to HIV-related non-Hodgkin lymphoma. Haematologica, 106(8): 2233-2241. https://doi.org/10.3324/haematol.2020.247023
- Lalli, M., Yen, A., Thopte, U., Dong, F., Moudgil, A., Chen, X., Milbrandt, J., Dougherty, Joseph D., & Mitra, Robi D. 2022. Measuring transcription factor binding and gene expression using barcoded self-reporting transposon calling cards and transcriptomes. NAR Genomics and Bioinformatics, 4(3). https://doi.org/10.1093/nargab/lqac061
- Li, Y., Schwalie, P. C., Bast-Habersbrunner, A., Mocek, S., Russeil, J., Fromme, T., Deplancke, B., & Klingenspor, M. 2019. Systems-Genetics-Based Inference of a Core Regulatory Network Underlying White Fat Browning. Cell Reports, 29(12): 4099-4113.e4095. https://doi.org/10.1016/j.celrep.2019.11.053
- Litovchenko, M., Meireles-Filho, A. C. A., Frochaux, M. V., Bevers, R. P. J., Prunotto, A., Anduaga, A. M., Hollis, B., Gardeux, V., Braman, V. S., Russeil, J. M. C., Kadener, S., dal Peraro, M., & Deplancke, B. 2021. Extensive tissue-specific expression variation and novel regulators underlying circadian behavior. Science Advances, 7(5): eabc3781. https://doi.org/10.1126/sciadv.abc3781

Human

Number of samples: 96

Reads per sample in demo dataset:

10'000 reads

To have access to the deep-sequenced dataset (9.5M reads per sample) contact us.

Demo dataset file size:

79.9 MB

Mouse

Number of samples: 96

Reads per sample in demo dataset:

10'000 reads

To have access to the deep-sequenced dataset (8.9M reads per sample) contact us.

Demo dataset file size:

78.6 MB

Cattle

Number of samples: 54

Reads per sample in demo dataset:

10'000 reads

To have access to the deep-sequenced dataset (5.7M reads per sample) contact us.

Demo dataset file size:

28.4 MB

Wild boar

Number of samples: 54

Reads per sample in demo dataset:

10'000 reads

To have access to the deep-sequenced dataset (6.3M reads per sample) contact us.

Demo dataset file size:

27.7 MB

Bat

Number of samples: 23

Reads per sample in demo dataset:

10'000 reads

To have access to the deep-sequenced dataset (9M reads per sample), contact us.

Demo dataset file size:

23.3 MB
Arabidopsis

Number of samples: 96

Reads per sample in demo dataset:

10'000 reads

To have access to the deep-sequenced dataset (3M reads per sample), contact us.

Demo dataset file size:

47.9 MB

Spinach

Number of samples: 96

Reads per sample in demo dataset:

10'000 reads

To have access to the deep-sequenced dataset (9M reads per sample) contact us.

Demo dataset file size:

83.9 MB
Drosophila

Number of samples: 96

Reads per sample in demo dataset:

10'000 reads

To have access to the deep-sequenced dataset (3M reads per sample), contact us.

Demo dataset file size:

38.2 MB
Zebrafish

Number of samples: 64

Reads per sample in demo dataset:

10'000 reads

To have access to the deep-sequenced dataset (4M reads per sample) contact us.

Demo dataset file size:

31.9 MB

Rainbow trout

Number of samples: 16

Reads per sample in demo dataset:

10'000 reads

To have access to the deep-sequenced dataset (5.3M reads per sample) contact us.

Demo dataset file size:

8.76 MB

Medaka

Number of samples: 96

Reads per sample in demo dataset:

10'000 reads

To have access to the deep-sequenced dataset (4.7M reads per sample) contact us.

Demo dataset file size:

50.6 MB

Each BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.

To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
The recommended range of RNA amount for each sample is of 50ng-1μg, normally the more RNA, the better.

The minimum recommended RIN number is 6 and the A260/230 ratio (Nanodrop) should be in the 1.5-2.2 range.
The only difference between BRB-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the BRB-seq barcode sequence.

For a thorough description of BRB-seq data processing, please refer to the BRB-seq kit user guide.
One of the key advantages of BRB-seq is that it does not only save reagents and cost in the library preparation stage, but also in the sequencing one.

As opposed to standard RNA-seq, where 20M-30M reads per sample are required, we normally recommend to sequence BRB-seq libraries at a depth of 4M-5M reads per sample, which is normally enough to detect the vast majority of expressed genes.
The barcode set for your kit is conveniently located on the kit label. Please refer to the label for accurate identification.

For optimal compatibility, ensure that you use the appropriate plate format (e.g., for kits designed for 96 reactions, the 96 well-plate format should be used). This ensures accurate and efficient processing of your samples. If you have any further questions or concerns, please contact our support team for assistance by email or using our live chat tool.

MERCURIUS™

BRB-seq kits

Library preparation kits for Illumina^®, AVITI™ and MGI*