|Cat #10812||Cat #10813||Cat #11013||Cat #10814||Cat #11014|
|Total reactions How many library preps can be prepared in total with one kit||24||96||384||384||1536|
|RNA multiplexing format How many samples can be multiplexed in one single tube||24||96||96||384||384|
|UDI pairs included How many individual BRB-seq libraries can be pooled and sequenced together||1||4||4||4||4|
The MERCURIUS™ BRB-seq library preparation kits for Illumina® contain all the oligos and enzymes needed to go from purified RNA to sequencing-ready DNA libraries.
Bulk RNA sequencing at scale
Perform up-to 384 RNA-seq library preparations in one single tube.
Streamlined data pre-processing
Demultiplex and align your BRB-seq data with our easy-to-use cloud-based platform.
BRB-seq as a flexible solution
For projects of all sizes:
from 24 to 1536 reactions in one kit.
One-day lab workflow
Convenient and short protocol from samples to sequencing-ready libraries in one day.
Experimental workflow at a glance
1. Reverse Transcription
2. Sample Pooling
3. Second strand synthesis
5. Library indexing and amplification
6. Library QC and sequencing
7. Sequencing data processing and analysis
Each BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.
To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
The recommended range of RNA amount for each sample is of 50ng-1μg, normally the more RNA, the better.
The minimum recommended RIN number is 6 and the A260/230 ratio (Nanodrop) should be in the 1.5-2.2 range.
The only difference between BRB-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the BRB-seq barcode sequence.
For a thorough description of BRB-seq data processing, please refer to the BRB-seq kit user guide. In order to facilate as much as possible BRB-seq data analysis, we have also created a dedicate pipeline which can be found in our “SOFTWARE” page.
One of the key advantages of BRB-seq is that it does not only save reagents and cost in the library preparation stage, but also in the sequencing one.
As opposed to standard RNA-seq, where 20M-30M reads per sample are required, we normally recommend to sequence BRB-seq libraries at a depth of 4M-5M reads per sample, which is normally enough to detect the vast majority of expressed genes.
Speak with our RNA sequencing experts
Book a one-on-one call with one of our RNA experts to discover how we can assist your next project.