![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
|
---|---|---|---|---|---|---|
Total reactions
|
96 | 384 | 384 | 1'536 | 6'144 | 24'576 |
RNA and plate multiplexing format
|
96 | 96 | 384 | 384 | 384 | 384 |
Barcoded oligo-dT plates included | 1 | 4 | 1 | 4 | 16 | 64 |
UDI pairs included
|
4 | 4 | 4 | 4 | 16 | 16 |
Documentation |
Cat #10813
![]() |
||||
---|---|---|---|---|
Total reactions
|
96 | |||
RNA multiplexing format
|
96 | |||
UDI pairs included
|
4 | |||
Cat #11013
![]() |
||||
---|---|---|---|---|
Total reactions
|
384 | |||
RNA multiplexing format
|
96 | |||
UDI pairs included
|
4 | |||
Cat #10814
![]() |
||||
---|---|---|---|---|
Total reactions
|
384 | |||
RNA multiplexing format
|
384 | |||
UDI pairs included
|
4 | |||
Cat #11014
![]() |
||||
---|---|---|---|---|
Total reactions
|
||||
RNA multiplexing format
|
384 | |||
UDI pairs included
|
4 | |||
4x Cat #11014
![]() |
||||
---|---|---|---|---|
Total reactions
|
||||
RNA multiplexing format
|
384 | |||
UDI pairs included
|
16 | |||
16x Cat #11014
![]() |
||||
---|---|---|---|---|
Total reactions
|
||||
RNA multiplexing format
|
384 | |||
UDI pairs included
|
16 | |||
Benefits
The MERCURIUS™ BRB-seq library preparation kits for Illumina® contain all the oligos and enzymes needed to go from purified RNA to sequencing-ready DNA libraries.
Bulk RNA sequencing at scale
Perform up-to 384 RNA-seq library preparations in one single tube.
BRB-seq as a flexible solution
For projects of all sizes:
from 96 to 24'576 reactions in one kit.
All-inclusive.
One-day lab workflow
Convenient and short protocol from samples to sequencing-ready libraries in one day.
Experimental workflow at a glance

Performance
Uniform detection of 12'000+ genes at 1 million reads per sample across 384 samples

Distribution of the number of detected genes across...
Comparison of library complexity between BRB-seq and TruSeq at a comparable sequencing depth

Detected genes (left) and differentially expressed...
Benchmarking the performance of BRB-seq for DE genes at 3M reads/sample with other commercial platforms

Number of detected genes for the non-differentiated...
Benchmarking the performance for library prep between BRB-seq and two other commercial kits

Genome-wide Pearson correlation of mapped reads for...
4000+ DE genes with 3M reads/sample at FDR 0.5

Number of differentially expressed genes across three...
Uniform detection of 12'000+ genes at 1 million reads per sample across 384 samples

Distribution of the number of detected genes across 384 samples prepared with the MERCURIUS™ BRB-seq library preparation kit. The library was sequenced at an average of 1 million reads
Comparison of library complexity between BRB-seq and TruSeq at a comparable sequencing depth

Detected genes (left) and differentially expressed genes (right) obtained on the same samples with BRB-seq and TruSeq. At comparable sequencing depth, the performance of BRB-seq data is comparable with TruSeq libraries.
Benchmarking the performance of BRB-seq for DE genes at 3M reads/sample with other commercial platforms

Number of detected genes for the non-differentiated (ND) and differentiated (D) Adipose Stromal Population Cells between BRB-seq and two other commercial kits. The expressed genes are split into three categories: Lowly Expressed (left, 0 < CPM < 10), Mid expressed (middle, 10 < CPM < 100), and Highly expressed (right, CPM > 100). To note: all replicates were prepared from the same RNA sample and the sequencing depth was 3M reads/sample.
Benchmarking the performance for library prep between BRB-seq and two other commercial kits

Genome-wide Pearson correlation of mapped reads for the non-differentiated (ND) and differentiated (D) Adipose Stromal Population Cells between BRB-seq and two other commercial kits. A high correlation was observed both within technical replicates and across different library prep kits.
4000+ DE genes with 3M reads/sample at FDR 0.5

Number of differentially expressed genes across three commercial platforms, including BRB-seq, at different FDR cutoff values. To note: all replicates were prepared from the same RNA sample and the sequencing depth was 3M reads/sample.
Product Specifications
Sample datasets
-
Human
Number of samples: 96Reads per sample in demo dataset:10'000 readsTo have access to the deep-sequenced dataset (9.5M reads per sample) contact us.Demo dataset file size:79.9 MBMouse
Number of samples: 96Reads per sample in demo dataset:10'000 readsTo have access to the deep-sequenced dataset (8.9M reads per sample) contact us.Demo dataset file size:78.6 MBCattle
Number of samples: 54Reads per sample in demo dataset:10'000 readsTo have access to the deep-sequenced dataset (5.7M reads per sample) contact us.Demo dataset file size:28.4 MBWild boar
Number of samples: 54Reads per sample in demo dataset:10'000 readsTo have access to the deep-sequenced dataset (6.3M reads per sample) contact us.Demo dataset file size:27.7 MBBat
Number of samples: 23Reads per sample in demo dataset:10'000 readsTo have access to the deep-sequenced dataset (9M reads per sample), contact us.Demo dataset file size:23.3 MB -
Arabidopsis
Number of samples: 96Reads per sample in demo dataset:10'000 readsTo have access to the deep-sequenced dataset (3M reads per sample), contact us.Demo dataset file size:47.9 MBSpinach
Number of samples: 96Reads per sample in demo dataset:10'000 readsTo have access to the deep-sequenced dataset (9M reads per sample) contact us.Demo dataset file size:83.9 MB -
Drosophila
Number of samples: 96Reads per sample in demo dataset:10'000 readsTo have access to the deep-sequenced dataset (3M reads per sample), contact us.Demo dataset file size:38.2 MB -
Zebrafish
Number of samples: 64Reads per sample in demo dataset:10'000 readsTo have access to the deep-sequenced dataset (4M reads per sample) contact us.Demo dataset file size:31.9 MBRainbow trout
Number of samples: 16Reads per sample in demo dataset:10'000 readsTo have access to the deep-sequenced dataset (5.3M reads per sample) contact us.Demo dataset file size:8.76 MBMedaka
Number of samples: 96Reads per sample in demo dataset:10'000 readsTo have access to the deep-sequenced dataset (4.7M reads per sample) contact us.Demo dataset file size:50.6 MB
FAQs
-
Each BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.
To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
-
The recommended range of RNA amount for each sample is of 50ng-1μg, normally the more RNA, the better.
The minimum recommended RIN number is 6 and the A260/230 ratio (Nanodrop) should be in the 1.5-2.2 range.
-
The only difference between BRB-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the BRB-seq barcode sequence.
For a thorough description of BRB-seq data processing, please refer to the BRB-seq kit user guide.
-
One of the key advantages of BRB-seq is that it does not only save reagents and cost in the library preparation stage, but also in the sequencing one.
As opposed to standard RNA-seq, where 20M-30M reads per sample are required, we normally recommend to sequence BRB-seq libraries at a depth of 4M-5M reads per sample, which is normally enough to detect the vast majority of expressed genes.
Speak with our RNA sequencing experts
Book a one-on-one call with one of our RNA experts to discover how we can assist your next project.