MERCURIUS™

Targeted Plant BRB-seq service

Targeted RNA-seq combined with massive sample multiplexing.

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Benefits

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Custom-developed panels
Users communicate their targets of interest, and we will develop ad-hoc detection panels.
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Up to 100 targets

Experimentally validated panels for differential expression analysis.
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Dedicated service to plant samples
All the reagents used are validated and QC'ed on plant RNA.
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Minimal sequencing cost
By only sequencing the targets, sequencing costs are drastically decreased.
MERCURIUSTM

Targeted Plant BRB-seq service

The MERCURIUS™ Targeted Plant BRB-seq service offers the most comprehensive, high-throughput and cost-efficient solution for targeted gene expression analysis projects.

As part of the Targeted Plant BRB-seq service, clients share their genes of interest with us, we develop a corresponding custom detection panel and validate it directly on their purified RNA samples. 

Once the validation is complete, we will inform the client that the dedicated service is correctly established and that we are ready to receive their test samples.

By combining the advantages of the targeted approach with the BRB-seq system, up to 100 different genes can be assessed across tens to thousands of samples in parallel, significantly reducing the time and costs associated with library preparation and sequencing.

As a result of our service, our clients receive raw sequencing data (fastq files), gene count matrices and analysis reports files.

Optional downstream differential gene expression analysis are also available upon request.

BRB-seq service in action

How it works

Step 1

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Client communicates the genes of interest panel and ships representative purified RNA samples for the panel validation

Step 2

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Alithea develops and validates the custom detection panel on customer's samples - Client Checkpoint

time@100x-50 4 to 8 weeks

 

Step 3

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Client ships purified RNA samples for Targeted BRB-seq library prep

Step 4

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Incoming QC - Client Checkpoint

(Nanodrop and Fragment Analyzer)

 

time 1 week

Step 5

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MERCURIUS™ Targeted BRB-seq library prep for Illumina and AVITI

time 2 days

Step 6

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Library QC - Client Checkpoint

(Qubit, Fragment analyzer)

 

 

 

time 1 week

Step 7

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Sequencing - Illumina / AVITI

 

 

time 1.5 weeks

Step 8

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Data analysis and reporting - Client Checkpoint

 

 

time 1 week

Step 9

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Data

Optional: Alithea performs Differential Gene Expression analysis​

Performance

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Improvement of target genes detection with Targeted BRB-seq

Gene ranked by detection abundance with MERCURIUS™ Targeted BRB-seq on a panel of 24 target genes compared to MERCURIUS™ BRB-seq.

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Enrichment of UMIs related to targeted genes

Targeted BRB-seq shows greater enrichment for targeted genes. 

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Relative enrichment of targeted genes between Targeted BRB-seq and BRB-seq

Scattered plot showing the relative identification of genes (blue dots: Targeted BRB-seq, grey dots: BRB-seq). The targeted approach preferentially identifies the targeted genes.

FAQs

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Relevant publications

Explore the latest, relevant publications in the industry to learn more about our technologies.

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Want to do Targeted Plant BRB-seq in-house?

Our Targeted Plant BRB-seq kits make it possible to process virtually any number of RNA samples in one single tube, which helps reduce technical variation, turnaround times and costs.
Experience sample to sequencing-ready library in one day.

Speak with one of our RNA experts

Book a one-on-one call with one of our RNA experts to discover how we can assist your next project.

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