Targeted Plant BRB-seq service
Targeted RNA-seq combined with massive sample multiplexing.
Targeted Plant BRB-seq service
The MERCURIUS™ Targeted Plant BRB-seq service offers the most comprehensive, high-throughput and cost-efficient solution for targeted gene expression analysis projects.
As part of the Targeted Plant BRB-seq service, clients share their genes of interest with us, we develop a corresponding custom detection panel and validate it directly on their purified RNA samples.
Once the validation is complete, we will inform the client that the dedicated service is correctly established and that we are ready to receive their test samples.
By combining the advantages of the targeted approach with the BRB-seq system, up to 100 different genes can be assessed across tens to thousands of samples in parallel, significantly reducing the time and costs associated with library preparation and sequencing.
As a result of our service, our clients receive raw sequencing data (fastq files), gene count matrices and analysis reports files.
Optional downstream differential gene expression analysis are also available upon request.
Step 1
Step 2
Step 3
Step 4
(Nanodrop and Fragment Analyzer)
1 week
Step 5
Step 6
(Qubit, Fragment analyzer)
1 week
Step 7
1.5 weeks
Step 8
1 week
Step 9
Gene ranked by detection abundance with MERCURIUS™ Targeted BRB-seq on a panel of 24 target genes compared to MERCURIUS™ BRB-seq.
Targeted BRB-seq shows greater enrichment for targeted genes.
Scattered plot showing the relative identification of genes (blue dots: Targeted BRB-seq, grey dots: BRB-seq). The targeted approach preferentially identifies the targeted genes.
In addition to total RNA amount, it is important that the samples contain RNA of high integrity (RIN > 6) and are devoid of contaminants (Nanodrop A260/A230 between 1.8 and 2.2).
Targeted Plant BRB-seq is a 3’-end RNA sequencing method that requires significantly less sequencing than standard BRB-seq to achieve accurate gene quantification. Therefore, we recommend sequencing 100 reads per target for each sample, which enables reliable detection of the targeted genes.
As part of our standard service pipeline, we align the generated data to the genome of choice, provide a detailed report on the alignment and gene counting statistics and, finally, provide ready-to-use gene count matrices for downstream analysis.
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