MERCURIUS™

Blood BRB-seq service

By leveraging BRB-seq, we not only provide to industrial and academic clients high quality RNA-seq data, but we also do so with the highest affordability and shortest turnaround times on the market.

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Benefits

The generated 3' mRNA-seq data is ideally suited for differential gene expression analysis.

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Bulk RNA sequencing at scale
Unparalleled cost and throughput scalability.
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Ideal for projects of all sizes
Run projects for as many as 100,000 samples.
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Fast turnaround times

From samples to data in as little as six weeks.
MERCURIUSTM

Blood BRB-Seq service

The MERCURIUS ™ Blood BRB-seq service offers a convenient and streamlined solution for your blood transcriptomics projects. We have expertise with different types of RNA purification from blood samples and we have specialised in high-throughput RNA extraction from PaxGene tubes.

Customers can therefore either send us directly their blood samples or perform RNA extraction in-house and send us purified RNA samples. Upon samples reception, we always perform incoming QC before launching our Blood BRBseq pipeline. During the process, we always keep clients informed at defined checkpoint so that we can decide together how to best proceed to the next steps.

Next generation sequecing and data preprocessing (including alignment to the genome of choice) are part of our standard service as well. As a result, we provide our clients raw data, sequencing and alignment reports, and gene count matrices which can be used for downstream gene expression analysis.

BRB-seq service in action

How it works

Step 1

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Client ships samples to Alithea

For blood samples, we offer high-throughput RNA extraction directly from PAXgene®, Tempus ™ Blood RNA and RNA shield tubes. 
 
 

Step 2

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Incoming QC - Client Checkpoint

(Nanodrop, Ribogreen, Tapestation)

time 1 week

 

Step 3

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MERCURIUS™ Blood BRB-seq library preparation

 

time 2 days

Step 4

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Library QC - Client Checkpoint

(Nanodrop and Fragment Analyzer)

time 1 week

Step 5

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Deep sequencing

 

 

time 1 week

Step 6

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Data analysis and reporting - Client Checkpoint

Step 7

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Data

Optional: Alithea performs Differential Gene Expression analysis​

Sample dataset

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Human

Number of samples:
96

Reads per sample in demo dataset:
10'000 reads

To have access to the deep-sequenced dataset (4.8M reads per sample) contact us.

Demo dataset file size:
57.7 MB

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FAQs

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Relevant publications

Explore the latest, relevant publications in the industry to learn more about our technologies.

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Want to do Blood BRB-seq in-house?

Our Blood BRB-seq kits make it possible to process virtually any number of RNA samples in one single tube, which helps reduce technical variation, turnaround times and costs.
Experience sample to sequencing-ready library in one day.

Speak with one of our RNA experts

Book a one-on-one call with one of our RNA experts to discover how we can assist your next project.

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