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MERCURIUS™

Blood BRB-seq service

By leveraging BRB-seq, we not only provide to industrial and academic clients high quality RNA-seq data, but we also do so with the highest affordability and shortest turnaround times on the market.

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Benefits

The generated 3' mRNA-seq data is ideally suited for differential gene expression analysis

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Bulk RNA sequencing at scale

Massively multiplexed RNA-seq offers unparalleled cost and throughput scalability.

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Ideal for projects of all sizes

You can trust us. We have ran projects from as low as 6 samples to as many as 100'000.

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Fast turnaround times

From samples to data in as little as one month.

What we do

MERCURIUS ™

Blood BRB-seq Service

The MERCURIUS ™ Blood BRB-seq service offers a convenient and streamlined solution for your blood transcriptomics projects. We have expertise with different types of RNA purification from blood samples and we have specialised in high-throughput RNA extraction from PaxGene tubes.

Customers can therefore either send us directly their blood samples or perform RNA extraction in-house and send us purified RNA samples. Upon samples reception, we always perform incoming QC before launching our Blood BRBseq pipeline. During the process, we always keep clients informed at defined checkpoint so that we can decide together how to best proceed to the next steps.

Next generation sequecing and data preprocessing (including alignment to the genome of choice) are part of our standard service as well. As a result, we provide our clients raw data, sequencing and alignment reports, and gene count matrices which can be used for downstream gene expression analysis.

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How does it work

  • Client ships samples to Alithea

    For blood samples, we offer high-throughput RNA extraction directly from PAXgene ®, Tempus ™ Blood RNA and RNA shield tubes
    Sample submission form for MERCURIUSTM Blood BRB-seq service (unpurified samples) Sample submission form for MERCURIUSTM Blood BRB-seq service (purified RNA)
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  • 1 week

    Incoming QC - Client Checkpoint

    (Nanodrop, Ribogreen, Tapestation)
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  • 2 days

    MERCURIUS™ Blood BRB-seq library preparation

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  • 1 week

    Library QC - Client Checkpoint

    (Qubit, Fragment analyzer, shallow sequencing)
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  • 1 week

    Deep sequencing

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  • 1 week

    Data analysis and reporting - Client Checkpoint

    Example of sample report
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  • Data

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  • Optional: Alithea performs Differential Gene Expression analysis​

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    Human

    Number of samples:
    96 
     
    Reads per sample in demo dataset:
    10'000 reads
     
    To have access to the deep-sequenced dataset (4.8M reads per sample) contact us.
     
    Demo dataset file size:
    57.7 MB
    Download

FAQs

  • To guarantee high quality data, we normally request that each sample contains at least 200ng of total RNA in at least 10μl.
  • In addition to total RNA amount, it is important that the samples contain RNA of high integrity (RIN > 6) and are devoid of contaminants (Nanodrop A260/A230 between 1.8 and 2.2).
  • BRB-seq is 3’-end RNA sequencing method and, as such, requires significantly less sequencing as compared to standard full-length RNA-seq in order to reach accurate gene quantification. We therefore normally recommend to sequence 4 to 5 million reads for each sample, which enables the reliable and unbiased detection of over 18’000 genes.
  • As part of our standard service pipeline, we align the generated data to the genome of choice, provide a detailed report on the alignment and gene counting statistics and, finally, provide ready-to-use gene count matrices for downstream analysis.

See our products

Our BRB-seq and DRUG-seq kits make it possible to process virtually any number of RNA samples in one single tube, which helps reduce technical variation, turnaround times and costs.

Experience sample to sequencing-ready library in one day.

Discover our kits
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BRB-seq was a great way for us to multiplex large numbers of RNA samples for sequencing. We wanted a gene expression curve with a tight temporal resolution, thus, many time points for several conditions. We are very happy with the quality of the results and with the cost benefit.

Dr. Nuno Miguel Luis, CNRS Researcher

BRB-seq is very cost-effective and it enables accurate comparison of RNA levels among a large number of samples.

Dr. Hirokazu Okada, ETH Zurich

BRB-seq allowed us to plan an experiment with ten times more replicates than usual, and gain insight into the variability and control of gene expression in fly embryogenesis

Prof. Dr. Marc Robinson-Rechavi, University of Lausanne

BRB-seq provided us with a cost-effective way to quantify transcriptomic changes from a large set of samples.

Prof. Dr. Martin Klingenspor, TUM Munich

Speak with our RNA sequencing experts

Need guidance or have questions about BRB-seq and our service?

Easily book a call with our BRB-seq experts.