MERCURIUS™ Blood BRB-seq-kits

	Cat #10823	Cat #11023	Cat #10825	Cat #11025
Total reactions How many library preps can be prepared in total with one kit	96	384	384	1'536
RNA multiplexing format How many samples can be multiplexed in one single tube	96	96	384	384
UDI pairs included How many individual BRB-seq libraries can be pooled and sequenced together	4	4	4	4
Globin blockers	included	included	included	included
Data analysis
Manual	Download Manual

Cat #10823
Total reactions How many library preps can be prepared in total with one kit	96
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4
Globin blockers	included

Download Manual

Cat #11023
Total reactions How many library preps can be prepared in total with one kit	384
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4
Globin blockers	included

Download Manual

Cat #10825
Total reactions How many library preps can be prepared in total with one kit	384
RNA multiplexing format How many library preps can be prepared in total with one kit	384
UDI pairs included How many library preps can be prepared in total with one kit	4
Globin blockers	included

Download Manual

Cat #11025
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit	384
UDI pairs included How many library preps can be prepared in total with one kit	4
Globin blockers	included

Download Manual

More samples, more replicates. Robust results, significant discoveries.

Demultiplex and align your BRB-seq data with our easy-to-use cloud-based platform.

Seamlessly integrated globin depletion. No need to purchase additional kits.

Convenient and short protocol from samples to sequencing-ready libraries in one day.

Performance

Distribution of the number of detected genes across 96...

(left) Proportion of mapped reads assigned to globin...

Correlation plots between mapped reads in between...

(left) Read distribution to the main globin genes on...

For use with (application)

3' mRNA sequencing

For use with (equipment)

Illumina NGS instruments

Species compatibility

All eukaryotic species

Available formats

96, 384, 1'536, 6'144, 36'864 reactions

Shipping conditions

Dry ice

Storage conditions

-20C

Each BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.

To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
The recommended range of RNA amount for each sample is of 50ng-1μg, normally the more RNA, the better.

The minimum recommended RIN number is 6 and the A260/230 ratio (Nanodrop) should be in the 1.5-2.2 range.
The only difference between BRB-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the BRB-seq barcode sequence.

For a thorough description of BRB-seq data processing, please refer to the BRB-seq kit user guide. In order to facilate as much as possible BRB-seq data analysis, we have also created a dedicate pipeline which can be found in our “SOFTWARE” page.
One of the key advantages of BRB-seq is that it does not only save reagents and cost in the library preparation stage, but also in the sequencing one.

As opposed to standard RNA-seq, where 20M-30M reads per sample are required, we normally recommend to sequence BRB-seq libraries at a depth of 4M-5M reads per sample, which is normally enough to detect the vast majority of expressed genes.