MERCURIUS™ Blood BRB-seq-kits


Catalog number	#10823	#11023	#10825	#11025
Total preps How many library preps can be prepared in total with one kit	96	384	384	1'536
RNA and plate multiplexing format How many samples can be multiplexed in one single tube	96	96	384	384
Barcoded oligo-dT plates included	1	4	1	4
UDI pairs included How many individual libraries can be sequenced together	4	4	4	4
Globin blockers	included	included	included	included
Documentation	User guide		Barcode files

#10,823 Cat ##10823
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4
Globin blockers	included
Download Manual

Cat ##11023
Total reactions How many library preps can be prepared in total with one kit	384
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4
Globin blockers	included
Download Manual

Cat ##10825
Total reactions How many library preps can be prepared in total with one kit	384
RNA multiplexing format How many library preps can be prepared in total with one kit	384
UDI pairs included How many library preps can be prepared in total with one kit	4
Globin blockers	included
Download Manual

Cat ##11025
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit	384
UDI pairs included How many library preps can be prepared in total with one kit	4
Globin blockers	included
Download Manual

Compatible accessories

UDI expansion module

12 UDI pairs

Compatible with the MERCURIUS™ product line.
Flexible dual indexing solutions.

Benefits

The MERCURIUS™ Blood BRB-seq library preparation kits for Illumina^® contain all the oligos and enzymes needed to go from purified blood RNA to sequencing-ready DNA libraries.

benefits_bulk_gradient_64x64

benefits_workflow_gradient

Bulk RNA sequencing

at scale

Integrated

globin depletion

One-day lab

workflow

More samples, more replicates. Robust results, significant discoveries.

Seamlessly integrated globin depletion. No need to purchase additional kits.

Convenient and short protocol from samples to sequencing-ready libraries in one day.

Experimental workflow at a glance
Performance

1. Uniform detection of 15'000+ genes at 1 million reads per sample across 96 samples

Distribution of the number of detected genes across 96 blood RNA samples prepared with the MERCURIUS™ Blood BRB-seq library preparation kit. The library was sequenced at an average of 1 million reads per sample on an Illumina NextSeq 550.

2. The Blood BRB-seq provides state-of-the-art performance in terms of globin depletion efficiency

(left) Proportion of mapped reads assigned to globin genes (blue) and other genes (grey) for blood RNA samples without globin depletion and with globin depletion performed using ThermoFisher’s GLOBINclear™-Human Kit and the Blood BRB-seq method. (right) Number of detected genes across the three different conditions. Non-depleted libraries show a sharp decrease in performance, while BRB-seq and Globin Clear libraries both provide rich globin-free data.

3. Globin depletion is effective and specific to the target globin genes

Correlation plots between mapped reads in between non-depleted libraries and Blood BRB-seq (left) and Globin Clear-depleted libraries (right). For both methods, globin depletion is effective and specific to the target globin genes, with negligible off-target effects on other genes.

4. Blood BRB-seq provides effective and high-throughput inline depletion of globin genes

(left) Read distribution to the main globin genes on BRB-seq libraries WITHOUT globin depletion. The majority of mapped genes are associated with globin genes. (right) Read distribution to the main globin genes on BRB-seq libraries WITH globin depletion. The reads associated to globin genes are drastically reduced.

Product specifications

For (application)	3' mRNA sequencing
For use with (equipment)	Illumina NGS instruments
Species compatibility	Human and mouse
Available formats	96, 384, 1'536 reactions
Shipping conditions	Dry ice
Storage conditions	-20C

Human

Number of samples:

96

Reads per sample in demo dataset:

10'000 reads

To have access to the deep-sequenced dataset (4.8M reads per sample) contact us.

Demo dataset file size:

57.7 MB

Each BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.

To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
The recommended range of RNA amount for each sample is of 50ng-1μg, normally the more RNA, the better.

The minimum recommended RIN number is 6 and the A260/230 ratio (Nanodrop) should be in the 1.5-2.2 range.
The only difference between BRB-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the BRB-seq barcode sequence.

For a thorough description of BRB-seq data processing, please refer to the BRB-seq kit user guide.
One of the key advantages of BRB-seq is that it does not only save reagents and cost in the library preparation stage, but also in the sequencing one.

As opposed to standard RNA-seq, where 20M-30M reads per sample are required, we normally recommend to sequence BRB-seq libraries at a depth of 4M-5M reads per sample, which is normally enough to detect the vast majority of expressed genes.

MERCURIUS™

Blood BRB-seq

Library preparation kits for Illumina^®