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MERCURIUS™

Plant BRB-seq 

Library preparation kits for Illumina®

Validated and QC'ed on plant RNA samples. 96, 384 RNA-seq library preps in one tube.

Cat ##10713
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit 96
UDI pairs included How many library preps can be prepared in total with one kit 4
Cat ##11713
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit 96
UDI pairs included How many library preps can be prepared in total with one kit 4
Cat ##10714
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit 384
UDI pairs included How many library preps can be prepared in total with one kit 4
Cat ##11714
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit 384
UDI pairs included How many library preps can be prepared in total with one kit 4

Add-ons

Accessories

UDI expansion
module

 

 

Asset 1-Jun-21-2023-09-58-09-8501-AM

 

 

  • Compatible with the MERCURIUS™ product line.
  • 12 UDI pairs.   
  • Flexible dual indexing solutions. 

Package

Libraries
sequencing

€3

per million reads

 


  • Take advantage of our low sequencing price, which is enabled by the recurrent sequencing runs. 
  • Your libraries will be sequenced in large sequencing runs with other compatible BRB-seq and DRUG-seq libraries.

 

Package

Data
pre-processing

 €600 NOW €150

per 96 samples

 

  • We provide you with everything from raw data to gene count matrices and sequencing report.
  • Guaranteed turn-on time: 2 days. 

  • Benefits

    The MERCURIUS™ Plant BRB-seq library preparation kits for Illumina® contain all the oligos and enzymes needed to go from purified plant RNA to sequencing-ready DNA libraries.

    Asset 1-Sep-27-2023-03-10-57-5384-PM

     

    benefits_bulk_gradient-1

     

    benefits_flexible_gradient

     

    benefits_workflow_gradient

     

    Validated on plant samples Bulk RNA sequencing at scale BRB-seq as a flexible solution

    One-day lab workflow

    The production process and kit composition are optimized and QC'ed with plant RNA.

    Perform up-to 384 RNA-seq library preparations in one single tube.

    For projects of all sizes: from 96 to 24'576 reactions in one kit. All-inclusive.

    Convenient and short protocol from samples to sequencing-ready libraries in one day.

     

  • Experimental workflow at a glance

    BRB-seq-1

     

  • Performance

    1. Very high demultiplexing rate from raw data

    Asset 4@600x-2

    The demultiplexing rate of the pooled 96 samples using MERCURIUS™ Plant BRB-seq library preparation kit is as high as 98%. 

     

    2. Mapping rates as high as 86%

    Asset 2@600x-4

    Summary alignment of Arabidopsis data library prepared with the MERCURIUS™ Plant BRB-seq library preparation kit. 

     

    3. Uniform detection of 19'000+ genes at 3 million reads per sample across 96 samples

     

    Asset 2@600x-3

    Distribution of the number of detected genes across 96 samples prepared with the MERCURIUS™ Plant BRB-seq library preparation kit. The library was sequenced at an average of 3 million reads.

  • Product specifications

     

    For (application)

    3' mRNA sequencing

    For use with (equipment)

    Illumina NGS instruments

    Species compatibility

    All plant species

    Available formats

    96, 384, 1'536, 6'144 and 24'576 reactions

    Shipping conditions

    Dry ice

    Storage conditions

    -20C

     

  • Citations

    • , , , , , , ,,  Multiscale physiological responses to nitrogen supplementation of maize hybrids.
      Plant Physiology, 2023: 00: 1–21. https://doi.org/10.1093/plphys/kiad583
  • Asset 2-Jul-04-2023-02-21-34-5676-PM-2

    Arabidopsis

    Number of samples:          96 
     
    Reads per sample in demo dataset:
    10'000 reads
     
    To have access to the deep-sequenced dataset (3M reads per sample), contact us.
     
    Demo dataset file size:
     47.9 MB
    Download
    Asset 2-Jul-04-2023-02-21-34-5676-PM-2

    Spinach

    Number of samples:          96 
     
    Reads per sample in demo dataset:
    10'000 reads
     
    To have access to the deep-sequenced dataset (9M reads per sample) contact us.
     
    Demo dataset file size:
     83.9 MB
    Download

FAQs

  • Each Plant BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different Plant BRB-seq pools.

    To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.

  • The recommended range of RNA amount for each sample is of 50ng-1μg, normally the more RNA, the better.

    The minimum recommended RIN number is 6 and the A260/230 ratio (Nanodrop) should be in the 1.5-2.2 range.

  • The only difference between Plant BRB-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the BRB-seq barcode sequence.

     

  • One of the key advantages of Plant BRB-seq is that it does not only save reagents and cost in the library preparation stage, but also in the sequencing one.

    As opposed to standard RNA-seq, where 20M-30M reads per sample are required, we normally recommend sequencing Plant BRB-seq libraries at a depth of 4M-5M reads per sample, which is normally enough to detect the vast majority of expressed genes.

  • The barcode set for your kit is conveniently located on the kit label. Please refer to the label for accurate identification.

    For optimal compatibility, ensure that you use the appropriate plate format (e.g., for kits designed for 96 reactions, the 96 well-plate format should be used). This ensures accurate and efficient processing of your samples. If you have any further questions or concerns, please contact our support team for assistance by email or using our live chat tool.

Try our services

Our BRB-seq and DRUG-seq services deliver raw sequencing data (fastq files), gene count matrices and analysis report files. A cost-efficient option suitable for projects of all sizes.

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