MERCURIUS™ BRB-seq kit for Illumina®


Catalog number	#10713	#11713	#10714	#11714	4x #11714	16x #11714
Total preps How many library preps can be prepared in total with one kit	96	384	384	1'536	6'144	24'576
RNA and plate multiplexing format How many samples can be multiplexed in one single tube	96	96	384	384	384	384
Barcoded oligo-dT plates included	1	4	1	4	16	64
UDI pairs included How many individual libraries can be sequenced together	4	4	4	4	16	16
Documentation	User guide			Barcode files

Cat #10713
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Cat #11713
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Cat #10714
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit	384
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Cat #11714
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit	384
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Cat 4x #11714
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit	384
UDI pairs included How many library preps can be prepared in total with one kit	16
Download Manual

Cat 16x #11714
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit	384
UDI pairs included How many library preps can be prepared in total with one kit	16
Download Manual

Compatible accessories

UDI expansion module

12 UDI pairs

Compatible with the MERCURIUS™ product line.
Flexible dual indexing solutions.

Benefits

The MERCURIUS™ Plant BRB-seq library preparation kits for Illumina® contain all the oligos and enzymes needed to go from purified plant RNA to sequencing-ready DNA libraries.


Validated on plant samples	Bulk RNA sequencing at scale	BRB-seq as a flexible solution	One-day lab workflow
The production process and kit composition are optimized and QC'ed with plant RNA.	Perform up-to 384 RNA-seq library preparations in one single tube.	For projects of all sizes: from 96 to 24'576 reactions in one kit. All-inclusive.	Convenient and short protocol from samples to sequencing-ready libraries in one day.

Experimental workflow at a glance
Performance

1. Very high demultiplexing rate from raw data

The demultiplexing rate of the pooled 96 samples using MERCURIUS™ Plant BRB-seq library preparation kit is as high as 98%.

2. Mapping rates as high as 86%

Summary alignment of Arabidopsis data library prepared with the MERCURIUS™ Plant BRB-seq library preparation kit.

3. Uniform detection of 19'000+ genes at 3 million reads per sample across 96 samples

Distribution of the number of detected genes across 96 samples prepared with the MERCURIUS™ Plant BRB-seq library preparation kit. The library was sequenced at an average of 3 million reads.

Product specifications

For (application)	3' mRNA sequencing
For use with (equipment)	Illumina NGS instruments
Species compatibility	All plant species
Available formats	96, 384, 1'536, 6'144 and 24'576 reactions
Shipping conditions	Dry ice
Storage conditions	-20C

Citations
- , , , , , , ,, Multiscale physiological responses to nitrogen supplementation of maize hybrids.
  
  Plant Physiology, 2023: 00: 1–21. https://doi.org/10.1093/plphys/kiad583

Arabidopsis

Number of samples: 96

Reads per sample in demo dataset:

10'000 reads

To have access to the deep-sequenced dataset (3M reads per sample), contact us.

Demo dataset file size:

47.9 MB

Spinach

Number of samples: 96

Reads per sample in demo dataset:

10'000 reads

To have access to the deep-sequenced dataset (9M reads per sample) contact us.

Demo dataset file size:

83.9 MB

Each Plant BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different Plant BRB-seq pools.

To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
The recommended range of RNA amount for each sample is of 50ng-1μg, normally the more RNA, the better.

The minimum recommended RIN number is 6 and the A260/230 ratio (Nanodrop) should be in the 1.5-2.2 range.
The only difference between Plant BRB-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the BRB-seq barcode sequence.
One of the key advantages of Plant BRB-seq is that it does not only save reagents and cost in the library preparation stage, but also in the sequencing one.

As opposed to standard RNA-seq, where 20M-30M reads per sample are required, we normally recommend sequencing Plant BRB-seq libraries at a depth of 4M-5M reads per sample, which is normally enough to detect the vast majority of expressed genes.

MERCURIUS™

Plant BRB-seq

Library preparation kits for Illumina^®