UDI expansion module
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Compatible with the MERCURIUS™ product line.
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12 UDI pairs.
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Flexible dual indexing solutions.
Library preparation kits for Illumina®, and AVITI™
Validated and QC'ed on plant RNA samples. 96, 384 RNA-seq library preps in one tube.
Catalog number | #10713 |
#11713 |
#10714 |
#11714 |
Total preps
How many library preps can be prepared in total with one kit
|
96 | 384 | 384 | 1,536 |
Sample multiplexing and plate format
How many samples can be multiplexed in one single tube
|
96 | 96 | 384 | 384 |
Barcoded oligo-dT plates included
|
1 | 4 | 1 | 4 |
UDI pairs included
How many individual libraries can be sequenced together
|
4 | 4 | 4 | 4 |
Documentation |
Cat ##10713 | ||||
---|---|---|---|---|
Total reactions
How many library preps can be prepared in total with one kit
|
96 | |||
RNA multiplexing format
How many library preps can be prepared in total with one kit
|
96 | |||
UDI pairs included
How many library preps can be prepared in total with one kit
|
4 | |||
Cat ##11713 | ||||
---|---|---|---|---|
Total reactions
How many library preps can be prepared in total with one kit
|
384 | |||
RNA multiplexing format
How many library preps can be prepared in total with one kit
|
96 | |||
UDI pairs included
How many library preps can be prepared in total with one kit
|
4 | |||
Cat ##10714 | ||||
---|---|---|---|---|
Total reactions
How many library preps can be prepared in total with one kit
|
384 | |||
RNA multiplexing format
How many library preps can be prepared in total with one kit
|
384 | |||
UDI pairs included
How many library preps can be prepared in total with one kit
|
4 | |||
Cat ##11714 | ||||
---|---|---|---|---|
Total reactions
How many library preps can be prepared in total with one kit
|
||||
RNA multiplexing format
How many library preps can be prepared in total with one kit
|
384 | |||
UDI pairs included
How many library preps can be prepared in total with one kit
|
4 | |||
Compatible with the MERCURIUS™ product line.
12 UDI pairs.
Flexible dual indexing solutions.
The MERCURIUS™ Plant BRB-seq library preparation kits for Illumina® and AVITI™ contain all the oligos and enzymes needed to go from purified plant RNA to sequencing-ready libraries.
The production process and kit composition are optimized and QC'ed with plant RNA.
1. Very high demultiplexing rate from raw data
The demultiplexing rate of the pooled 96 samples using MERCURIUS™ Plant BRB-seq library preparation kit is as high as 98%.
2. Mapping rates as high as 86%
Summary alignment of Arabidopsis data library prepared with the MERCURIUS™ Plant BRB-seq library preparation kit.
3. Uniform detection of 19'000+ genes at 3 million reads per sample across 96 samples
Distribution of the number of detected genes across 96 samples prepared with the MERCURIUS™ Plant BRB-seq library preparation kit. The library was sequenced at an average of 3 million reads.
For (application) |
3' mRNA sequencing |
For use with (equipment) |
Illumina and AVITI NGS instruments |
Species compatibility |
All plant species |
Available formats |
96, 384 and 1'536 preps |
Shipping conditions |
Dry ice |
Storage conditions |
-20C |
Sheng Ying, Brandon Webster, Lina Gomez-Cano, Kiran-Kumar Shivaiah, Qianjie Wang, Linsey Newton, Erich Grotewold, Addie Thompson, Peter K Lundquist. Multiscale physiological responses to nitrogen supplementation of maize hybrids.
Plant Physiology, 2023: 00: 1–21. https://doi.org/10.1093/plphys/kiad583
1. Very high demultiplexing rate from raw data
The demultiplexing rate of the pooled 96 samples using MERCURIUS™ Plant BRB-seq library preparation kit is as high as 98%.
2. Mapping rates as high as 86%
Summary alignment of Arabidopsis data library prepared with the MERCURIUS™ Plant BRB-seq library preparation kit.
3. Uniform detection of 19'000+ genes at 3 million reads per sample across 96 samples
Distribution of the number of detected genes across 96 samples prepared with the MERCURIUS™ Plant BRB-seq library preparation kit. The library was sequenced at an average of 3 million reads.
For (application) |
3' mRNA sequencing |
For use with (equipment) |
Illumina and AVITI NGS instruments |
Species compatibility |
All plant species |
Available formats |
96, 384 and 1'536 preps |
Shipping conditions |
Dry ice |
Storage conditions |
-20C |
Sheng Ying, Brandon Webster, Lina Gomez-Cano, Kiran-Kumar Shivaiah, Qianjie Wang, Linsey Newton, Erich Grotewold, Addie Thompson, Peter K Lundquist. Multiscale physiological responses to nitrogen supplementation of maize hybrids.
Plant Physiology, 2023: 00: 1–21. https://doi.org/10.1093/plphys/kiad583
Number of samples:
96
Reads per sample in demo dataset:
10'000 reads
To have access to the deep-sequenced dataset (3M reads per sample), contact us.
Demo dataset file size:
47.9 MB
Number of samples:
96
Reads per sample in demo dataset:
10'000 reads
To have access to the deep-sequenced dataset (9M reads per sample) contact us.
Demo dataset file size:
83.9 MB
Each Plant BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different Plant BRB-seq pools.
To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
The recommended range of RNA amount for each sample is of 50ng-1μg, normally the more RNA, the better.
The minimum recommended RIN number is 7 and the A260/230 ratio (Nanodrop) should be in the 1.5-2.2 range.
The only difference between Plant BRB-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the BRB-seq barcode sequence.
One of the key advantages of Plant BRB-seq is that it does not only save reagents and cost in the library preparation stage, but also in the sequencing one.
As opposed to standard RNA-seq, where 20M-30M reads per sample are required, we normally recommend sequencing Plant BRB-seq libraries at a depth of 4M-5M reads per sample, which is normally enough to detect the vast majority of expressed genes.
The barcode set for your kit is conveniently located on the kit label. Please refer to the label for accurate identification.
For optimal compatibility, ensure that you use the appropriate plate format (e.g., for kits designed for 96 reactions, the 96 well-plate format should be used). This ensures accurate and efficient processing of your samples. If you have any further questions or concerns, please contact our support team for assistance by email or using our live chat tool.
Explore the latest, relevant publications in the industry to learn more about our technologies.
Our Plant BRB-seq services delivers raw sequencing data (fastq files), gene count matrices and analysis report files. A cost-efficient option suitable for projects of all sizes.
Book a one-on-one call with one of our RNA experts to discover how we can assist your next project.
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