Full-Length DRUG-seq service
Massively multiplexed and full-length RNA-seq directly on cell, organoid and tissue lysates - no need for prior RNA extraction.
The MERCURIUS™ Full-Length DRUG-seq service offers a convenient and streamlined solution for full-length mRNA transcriptomics projects of any size.
As part of the Full-Length DRUG-seq service, our clients simply needs to deliver to us their 96- or 384-well plates containing frozen cells.
Upon receipt, our team will start processing the plates the same week and return results in as little as three weeks.
During the process, we always keep clients informed at defined checkpoints so that we can decide together how to best proceed to the next steps.
Next generation sequencing and data pre-processing (including alignment to the genome of choice) are part of our standard service as well. As a result, we provide our clients raw data, sequencing and alignment reports, and gene count matrices which can be used for downstream gene expression analysis.
Optional downstream differential gene expression analysis are also available upon request.
Step 1
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2 days
Step 5
(Qubit, Fragment analyzer, shallow sequencing)
1 week
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1 week
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Step 8
To generate high-quality sequencing data we recommend starting 5’000 – 15’000 mammalian cells per well.
The total RNA amount per pool should be at least 80'000 cells.
In addition to total RNA amount, it is important that the samples contain RNA of high integrity (RIN > 7) and are devoid of contaminants (Nanodrop A260/A230 between 1.8 and 2.2).
Full-Length DRUG-seq provides comprehensive coverage of the full-length mRNA transcripts.
We, therefore, normally recommend sequencing 5-20 million reads for each sample, depending on project scale and scope.
Explore the latest, relevant publications in the industry to learn more about our technologies.
Book a one-on-one call with one of our RNA experts to discover how we can assist your next project.