MERCURIUS™

BRB-seq service

By leveraging BRB-seq, we not only provide to industrial and academic clients high quality RNA-seq data, but we also do so with the highest affordability and shortest turnaround times on the market.

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Benefits

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Bulk RNA sequencing at scale
Perform up-to 384 RNA-seq library preparations in one single tube.
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Ideal for projects of all sizes
We have experience with projects of all sizes: from 48 to 10'000+ samples.
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Fast turnaround times

From samples to data in as little as three weeks.
MERCURIUSTM

BRB-seq service

The MERCURIUS™ BRB-seq service offers a convenient and streamlined solution for transcriptomics projects of any size. Clients can send us purified RNA samples, which are always quality-checked before launching our BRB-seq pipeline. During the process, we always keep clients informed at defined checkpoint so that we can decide together how to best proceed to the next steps.

Next generation sequencing and data pre-processing (including alignment to the genome of choice) are part of our standard service as well. As a result, we provide our clients raw data, sequencing and alignment reports, and gene count matrices which can be used for downstream gene expression analysis.

BRB-seq in action

How it works

Step 1

Step 3

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Client ships samples to Alithea

Step 4

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Incoming QC - Client Checkpoint

(Nanodrop and Fragment Analyzer)

time 1 week

 

Step 5

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MERCURIUS™ BRB-seq library preparation

 

 

time 2 days

Step 6

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Library QC - Client Checkpoint

(Qubit, Fragment analyzer, shallow sequencing)

time 1 week

Step 7

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Deep sequencing

 

 

time 1 week

Step 8

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Data analysis and reporting - Client Checkpoint

Step 9

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Data

Optional: Alithea performs Differential Gene Expression analysis​

Sample datasets

Asset 5-3-1

Human

Number of samples:
96

Reads per sample in demo dataset:
10'000 reads

To have access to the deep-sequenced dataset (9.5M reads per sample) contact us.

Demo dataset file size:
79.9 MB

Download
Asset 5-3-1

Mouse

Number of samples:
96 

Reads per sample in demo dataset:
10'000 reads

To have access to the deep-sequenced dataset (8.9M reads per sample) contact us.

Demo dataset file size:
78.6 MB

Download
Asset 5-3-1

Cattle

Number of samples:
54

Reads per sample in demo dataset:
10'000 reads 

To have access to the deep-sequenced dataset (5.7M reads per sample) contact us. 

Demo dataset file size:
28.4 MB

Download
Asset 5-3-1

Wild Boar

Number of samples:
54

Reads per sample in demo dataset:
10'000 reads

To have access to the deep-sequenced dataset (6.3M reads per sample) contact us.

Demo dataset file size:
27.7 MB

Download
Asset 5-3-1

Bat

Number of samples:
23 

Reads per sample in demo dataset:
10'000 reads

To have access to the deep-sequenced dataset (9M reads per sample), contact us.

Demo dataset file size:
23.3 MB

Download

FAQs

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Relevant publications

Explore the latest, relevant publications in the industry to learn more about BRB-seq.

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Want to do BRB-seq in-house?

Our BRB-seq kits make it possible to process virtually any number of RNA samples in one single tube, which helps reduce technical variation, turnaround times and costs.
Experience sample to sequencing-ready library in one day.

Speak with one of our RNA experts

Book a one-on-one call with one of our RNA experts to discover how we can assist your next project.