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MERCURIUS™

Plant BRB-seq service

By leveraging BRB-seq, we not only provide to industrial and academic clients high-quality RNA-seq data, but we also do so with the highest affordability and shortest turnaround times on the market.

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Benefits

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Dedicated service for plant samples

All the reagents used are validated and QC'ed on plant RNA.

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Bulk RNA sequencing at scale

Massively multiplexed RNA-seq offers unparalleled cost and throughput scalability.

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Ideal for projects of all sizes

You can trust us. We have ran projects from as low as 6 samples to as many as 100'000.

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Fast turnaround times

From samples to data in as fast as 1.5 months.

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Flexible genome choice

We align your data to the genome of interest.

What we do

MERCURIUS™

Plant BRB-seq service

The MERCURIUS™ Plant BRB-seq service offers a convenient and streamlined solution for transcriptomics projects of any size. Clients can send us purified plant RNA samples, which are always quality-checked before launching our Plant BRB-seq pipeline. During the process, we always keep clients informed at defined checkpoint so that we can decide together how to best proceed to the next steps.

Next generation sequencing and data pre-processing (including alignment to the genome of choice) are part of our standard service as well. As a result, we provide our clients raw data, sequencing and alignment reports, and gene count matrices which can be used for downstream gene expression analysis.

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How does it work

  • Client prepares the samples

    Sample preparation guidelines for purified RNA (PDF)
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  • Client sends submission form

    Sample submission form for MERCURIUSTM BRB-seq service
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  • Client ships samples to Alithea

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  • 1 week

    Incoming QC - Client Checkpoint

    (Nanodrop, Ribogreen, Tapestation)
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  • 2 days

    MERCURIUS™ Plant BRB-seq library preparation for Illumina®

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  • 1 week

    Library QC - Client Checkpoint

    (Qubit, Fragment analyzer, shallow sequencing)
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  • 1.5 weeks

    Deep sequencing - Illumina

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  • 1 week

    Data analysis and reporting - Client Checkpoint

    Example of sample report
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  • Data

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  • Optional: Alithea performs Differential Gene Expression analysis​

Performance

Sample datasets

  • Asset 2-Jul-04-2023-02-21-34-5676-PM-2

    Arabidopsis

    Number of samples:          96 
     
    Reads per sample in demo dataset:
    10'000 reads
     
    To have access to the deep-sequenced dataset (3M reads per sample), contact us.
     
    Demo dataset file size:
     47.9 MB
    Download
    Asset 2-Jul-04-2023-02-21-34-5676-PM-2

    Spinach

    Number of samples:          96 
     
    Reads per sample in demo dataset:
    10'000 reads
     
    To have access to the deep-sequenced dataset (9M reads per sample) contact us.
     
    Demo dataset file size:
     83.9 MB
    Download

FAQs

  • To guarantee high quality data, we normally request that each sample contains at least 200ng of total RNA in at least 10μl.
  • In addition to total RNA amount, it is important that the samples contain RNA of high integrity (RIN > 6) and are devoid of contaminants (Nanodrop A260/A230 between 1.8 and 2.2).
  • BRB-seq is 3’-end RNA sequencing method and, as such, requires significantly less sequencing as compared to standard full-length RNA-seq in order to reach accurate gene quantification. We therefore normally recommend to sequence 4 to 5 million reads for each sample, which enables the reliable and unbiased detection of over 18’000 genes.
  • As part of our standard service pipeline, we align the generated data to the genome of choice, provide a detailed report on the alignment and gene counting statistics and, finally, provide ready-to-use gene count matrices for downstream analysis.

    Optionally, we can include differential gene expression analysis.

See our products

Our BRB-seq and DRUG-seq kits make it possible to process virtually any number of RNA samples in one single tube, which helps reduce technical variation, turnaround times and costs.

Experience sample to sequencing-ready library in one day.

Discover our kits
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Speak with our RNA sequencing experts

Need guidance or have questions about BRB-seq and our service?

Easily book a call with our BRB-seq experts.