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MERCURIUS™

Plant BRB-seq service

By leveraging BRB-seq, we not only provide to industrial and academic clients high-quality RNA-seq data, but we also do so with the highest affordability and shortest turnaround times on the market.

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Benefits

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Dedicated service for plant samples

All the reagents used are validated and QC'ed on plant RNA.

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Bulk RNA sequencing at scale

Massively multiplexed RNA-seq offers unparalleled cost and throughput scalability.

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Ideal for projects of all sizes

You can trust us. We have ran projects from as low as 6 samples to as many as 100'000.

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Flexible genome choice

We align your data to the genome of interest.

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Fast turnaround times

From samples to data in as little as one month.

What we do

MERCURIUS™

Plant BRB-seq service

The MERCURIUS™ Plant BRB-seq service offers a convenient and streamlined solution for transcriptomics projects of any size. Clients can send us purified plant RNA samples, which are always quality-checked before launching our Plant BRB-seq pipeline. During the process, we always keep clients informed at defined checkpoint so that we can decide together how to best proceed to the next steps.

Next generation sequencing and data pre-processing (including alignment to the genome of choice) are part of our standard service as well. As a result, we provide our clients raw data, sequencing and alignment reports, and gene count matrices which can be used for downstream gene expression analysis.

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How does it work

  • Client prepares the samples

    Sample preparation guidelines for purified RNA (PDF)
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  • Client sends submission form

    Sample submission form for MERCURIUSTM Plant BRB-seq service
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  • Client ships samples to Alithea

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  • 1 week

    Incoming QC - Client Checkpoint

    (Nanodrop, Ribogreen, Tapestation)
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  • 2 days

    MERCURIUS™ Plant BRB-seq library preparation

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  • 1 week

    Library QC - Client Checkpoint

    (Qubit, Fragment analyzer, shallow sequencing)
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  • 1 week

    Deep sequencing

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  • 1 week

    Data analysis and reporting - Client Checkpoint

    Example of sample report
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  • Data

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  • Optional: Alithea performs Differential Gene Expression analysis​

Performance

Very high demultiplexing rate from raw data

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The demultiplexing rate of the pooled 96 samples using...

Mapping rates as high as 86%

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Summary alignment of Arabidopsis data library prepared...

Uniform detection of 19'000+ genes at 3 million reads per sample across 96 samples

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Distribution of the number of detected genes across 96...

Sample datasets

  • Asset 2-Jul-04-2023-02-21-34-5676-PM-2

    Arabidopsis

    Number of samples:          96 
     
    Reads per sample in demo dataset:
    10'000 reads
     
    To have access to the deep-sequenced dataset (3M reads per sample), contact us.
     
    Demo dataset file size:
     47.9 MB
    Download
    Asset 2-Jul-04-2023-02-21-34-5676-PM-2

    Spinach

    Number of samples:          96 
     
    Reads per sample in demo dataset:
    10'000 reads
     
    To have access to the deep-sequenced dataset (9M reads per sample) contact us.
     
    Demo dataset file size:
     83.9 MB
    Download

FAQs

  • To guarantee high quality data, we normally request that each sample contains at least 200ng of total RNA in at least 10μl.
  • In addition to total RNA amount, it is important that the samples contain RNA of high integrity (RIN > 6) and are devoid of contaminants (Nanodrop A260/A230 between 1.8 and 2.2).
  • BRB-seq is 3’-end RNA sequencing method and, as such, requires significantly less sequencing as compared to standard full-length RNA-seq in order to reach accurate gene quantification. We therefore normally recommend to sequence 4 to 5 million reads for each sample, which enables the reliable and unbiased detection of over 18’000 genes.

  • As part of our standard service pipeline, we align the generated data to the genome of choice, provide a detailed report on the alignment and gene counting statistics and, finally, provide ready-to-use gene count matrices for downstream analysis.

    Optionally, we can include differential gene expression analysis.

See our products

Our BRB-seq and DRUG-seq kits make it possible to process virtually any number of RNA samples in one single tube, which helps reduce technical variation, turnaround times and costs.

Experience sample to sequencing-ready library in one day.

Discover our kits
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BRB-seq was a great way for us to multiplex large numbers of RNA samples for sequencing. We wanted a gene expression curve with a tight temporal resolution, thus, many time points for several conditions. We are very happy with the quality of the results and with the cost benefit.

Dr. Nuno Miguel Luis, CNRS Researcher

BRB-seq is very cost-effective and it enables accurate comparison of RNA levels among a large number of samples.

Dr. Hirokazu Okada, ETH Zurich

BRB-seq allowed us to plan an experiment with ten times more replicates than usual, and gain insight into the variability and control of gene expression in fly embryogenesis

Prof. Dr. Marc Robinson-Rechavi, University of Lausanne

BRB-seq provided us with a cost-effective way to quantify transcriptomic changes from a large set of samples.

Prof. Dr. Martin Klingenspor, TUM Munich

Speak with our RNA sequencing experts

Need guidance or have questions about BRB-seq and our service?

Easily book a call with our BRB-seq experts.