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Targeted BRB-seq service

Targeted RNA-seq combined with massive sample multiplexing.

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Custom-developed panels

Users communicate their targets of interest, and we will develop ad-hoc detection panels.


Up to

Experimentally validated panels for differential expression analysis.


Minimal sequencing cost

By only sequencing the targets, sequencing costs are drastically decreased.

What we do


Targeted BRB-seq service

The MERCURIUS™ Targeted BRB-seq service offers the most comprehensive, high-throughput and cost-efficient solution for targeted gene expression analysis projects.

As part of the Targeted BRB-seq service, clients share their genes of interest with us, we develop a corresponding custom detection panel and validate it directly on their purified RNA samples. 

Once the validation is complete, we will inform the client that the dedicated service is correctly established and that we are ready to receive their test samples.

By combining the advantages of the targeted approach with the BRB-seq system, up to 100 different genes can be assessed across tens to thousands of samples in parallel, significantly reducing the time and costs associated with library preparation and sequencing.

As a result of our service, our clients receive raw sequencing data (fastq files), gene count matrices and analysis reports files.

Optional downstream differential gene expression analysis are also available upon request.


How does it work

  • Client communicates the genes of interest panel and ships representative purified RNA samples for the panel validation

    Sample submission guidelines for MERCURIUSTM  Targeted BRB-seq service Gene panel submission form for Targeted BRB-seq or Targeted DRUG-seq
  • 4 to 8 weeks

    Alithea developes and validate the custom detection panel on customer's samples - Client Checkpoint

  • Client ships purified RNA samples for Targeted BRB-seq library prep

    Sample submission form for MERCURIUSTM  Targeted BRB-seq service
  • 1 week

    Incoming QC - Client Checkpoint

    (Nanodrop, Ribogreen, Tapestation)
  • 2 days

    MERCURIUS™ Targeted BRB-seq library prep for Illumina and AVITI

  • 1 week

    Library QC - Client Checkpoint

    (Qubit, Fragment analyzer)
  • 1.5 weeks

    Sequencing - Illumina / AVITI

  • 1 week

    Data analysis and reporting - Client Checkpoint

  • Data

  • Optional: Alithea performs Differential Gene Expression analysis​



  • To guarantee high quality data, we normally request that each sample contains at least 200ng of total RNA in at least 10μl.
  • In addition to total RNA amount, it is important that the samples contain RNA of high integrity (RIN > 6) and are devoid of contaminants (Nanodrop A260/A230 between 1.8 and 2.2).
  • Targeted BRB-seq is a 3’-end RNA sequencing method that requires significantly less sequencing than standard BRB-seq to achieve accurate gene quantification. Therefore, we recommend sequencing 100 reads per target for each sample, which enables reliable detection of the targeted genes.
  • As part of our standard service pipeline, we align the generated data to the genome of choice, provide a detailed report on the alignment and gene counting statistics and, finally, provide ready-to-use gene count matrices for downstream analysis.

See our products

Our BRB-seq and DRUG-seq kits make it possible to process virtually any number of RNA samples in one single tube, which helps reduce technical variation, turnaround times and costs.

Experience sample to sequencing-ready library in one day.

Discover our kits

Speak with our RNA sequencing experts

Need guidance or have questions about DRUG-seq and our service?

Easily book a call with our RNA-seq experts.