Targeted BRB-seq service

Custom-developed panels

Users communicate their targets of interest, and we will develop ad-hoc detection panels.

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Up to
100
targets

Experimentally validated panels for differential expression analysis.

Minimal sequencing cost

By only sequencing the targets, sequencing costs are drastically decreased.

MERCURIUS ™

The MERCURIUS™ Targeted BRB-seq service offers the most comprehensive, high-throughput and cost-efficient solution for targeted gene expression analysis projects.

As part of the Targeted BRB-seq service, clients share their genes of interest with us, we develop a corresponding custom detection panel and validate it directly on their purified RNA samples.

Once the validation is complete, we will inform the client that the dedicated service is correctly established and that we are ready to receive their test samples.

By combining the advantages of the targeted approach with the BRB-seq system, up to 100 different genes can be assessed across tens to thousands of samples in parallel, significantly reducing the time and costs associated with library preparation and sequencing.

As a result of our service, our clients receive raw sequencing data (fastq files), gene count matrices and analysis reports files.

Optional downstream differential gene expression analysis are also available upon request.

Client communicates the genes of interest panel and ships representative purified RNA samples for the panel validation
4 to 8 weeks

Alithea developes and validate the custom detection panel on customer's samples - Client Checkpoint
Client ships purified RNA samples for Targeted BRB-seq library prep
1 week

Incoming QC - Client Checkpoint

(Nanodrop, Ribogreen, Tapestation)
2 days

MERCURIUS™ Targeted BRB-seq library prep for Illumina and AVITI
1 week

Library QC - Client Checkpoint

(Qubit, Fragment analyzer)
1.5 weeks

Sequencing - Illumina / AVITI
1 week

Data analysis and reporting - Client Checkpoint
Data
Optional: Alithea performs Differential Gene Expression analysis

Performance

Improvement of target genes detection with Targeted BRB-seq

Gene ranked by detection abundance with MERCURIUS™...

Enrichment of UMIs related to targeted genes

Targeted BRB-seq shows greater enrichment for targeted...

Relative enrichment of targeted genes between Targeted BRB-seq and BRB-seq

Scattered plot showing the relative identification of...

To guarantee high quality data, we normally request that each sample contains at least 200ng of total RNA in at least 10μl.
In addition to total RNA amount, it is important that the samples contain RNA of high integrity (RIN > 6) and are devoid of contaminants (Nanodrop A260/A230 between 1.8 and 2.2).
Targeted BRB-seq is a 3’-end RNA sequencing method that requires significantly less sequencing than standard BRB-seq to achieve accurate gene quantification. Therefore, we recommend sequencing 100 reads per target for each sample, which enables reliable detection of the targeted genes.
As part of our standard service pipeline, we align the generated data to the genome of choice, provide a detailed report on the alignment and gene counting statistics and, finally, provide ready-to-use gene count matrices for downstream analysis.

MERCURIUS™

Targeted BRB-seq service

Benefits

Custom-developed panels

Up to
100
targets

Minimal sequencing cost

What we do

MERCURIUS ™