Targeted BRB-seq service
Targeted RNA-seq combined with massive sample multiplexing.
MERCURIUS™
- Custom-developed panels
- Users communicate their targets of interest, and we will develop ad-hoc detection panels.
- Up to 100 targets
- Experimentally validated panels for differential expression analysis.
- Minimal sequencing cost
- By only sequencing the targets, sequencing costs are drastically decreased.
MERCURIUSTM
Targeted BRB-seq service
The MERCURIUS™ Targeted BRB-seq service offers the most comprehensive, high-throughput and cost-efficient solution for targeted gene expression analysis projects.
As part of the Targeted BRB-seq service, clients share their genes of interest with us, we develop a corresponding custom detection panel and validate it directly on their purified RNA samples.
Once the validation is complete, we will inform the client that the dedicated service is correctly established and that we are ready to receive their test samples.
By combining the advantages of the targeted approach with the BRB-seq system, up to 100 different genes can be assessed across tens to thousands of samples in parallel, significantly reducing the time and costs associated with library preparation and sequencing.
As a result of our service, our clients receive raw sequencing data (fastq files), gene count matrices and analysis reports files.
Optional downstream differential gene expression analysis are also available upon request.
How it works
Step 1
Client communicates the genes of interest panel and ships representative purified RNA samples for the panel validation
Step 2
Alithea develops and validates the custom detection panel on customer's samples - Client Checkpoint
4 to 8 weeks
Step 3
Incoming QC - Client Checkpoint
(Nanodrop and Fragment Analyzer)
1 week
Step 4
MERCURIUS™ Targeted BRB-seq library prep for Illumina and AVITI
2 daysStep 5
Library QC - Client Checkpoint
(Qubit, Fragment analyzer)
1 week
Step 6
Sequencing - Illumina / AVITI
1.5 weeks
Step 7
Data analysis and reporting - Client Checkpoint
1 week
Step 8
Data
FAQs
Relevant publications
Explore the latest, relevant publications in the industry to learn more about our technologies.
Want to do Targeted BRB-seq in-house?
Our Targeted BRB-seq kits make it possible to process virtually any number of RNA samples in one single tube, which helps reduce technical variation, turnaround times and costs.
Experience sample to sequencing-ready library in one day.
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