
Cell lysis module
- Optimized lysis buffer for complete lysis- directly from frozen cells, and efficient reverse transcription.
- Compatible with DRUG-seq library prep kits.
Massively multiplexed and full-length mRNA-seq directly on cell, organoid and tissue lysates - no need for prior RNA extraction
Compatible with or Illumina® and AVITI™
Catalog number | #10701 |
#11651 |
||
Total preps
|
96 | 384 | ||
Sample multiplexing and plate format
|
96 | 96 | ||
Barcoded oligo-dT plates included
|
1 | 4 | ||
UDI pairs included
|
4 | 4 | ||
Documentation |
Cat ##10701 | ||||
---|---|---|---|---|
Total reactions
|
96 | |||
RNA multiplexing format
|
96 | |||
UDI pairs included
|
4 | |||
Cat ##11651 | ||||
---|---|---|---|---|
Total reactions
|
384 | |||
RNA multiplexing format
|
96 | |||
UDI pairs included
|
4 | |||
1. Uniform detection of 22'000+ genes at 12 million reads per sample across 48 samples
Distribution of the number of detected genes across 48 samples prepared with the MERCURIUS™ Full-Length BRB-seq service. The library was sequenced at an average of 12 million reads.
2. Full-Length BRB-seq shows high demultiplexing rate and high mapping rate to exons
Full-Length BRB-seq performance shows 99% demultiplexing rate from raw data, 78% mapping rate, and 18% duplication rate of the 48 pooled samples and sequenced at 12 million reads per sample.
3. Mapping rates as high as 80% for different RNA inputs
Mapping rates are uniform across three RNA inputs tested: 1ug, 10ng, and 100ng. Human Universal Reference RNA was used to prepare mRNA Full-Length BRB libraries.
4. Full-Length BRB-seq exhibits reads distribution across the transcript’s entire length
5. >50'000 isoforms detected at 12M sequencing depth
Benchmarking the Full-Length BRB-seq protocol against competitor N shows similar performance for isoform detection. More than 50,000 isoforms can be detected at a sequencing depth of 12M reads per sample.
For (application) |
Full-Length mRNA sequencing |
For use with (equipment) |
Illumina and AVITI NGS instruments |
Species compatibility |
All eukaryotic species |
Available formats |
96 and 384 preps |
Shipping conditions |
Dry ice |
Storage conditions |
-20C |
1. Uniform detection of 22'000+ genes at 12 million reads per sample across 48 samples
Distribution of the number of detected genes across 48 samples prepared with the MERCURIUS™ Full-Length BRB-seq service. The library was sequenced at an average of 12 million reads.
2. Full-Length BRB-seq shows high demultiplexing rate and high mapping rate to exons
Full-Length BRB-seq performance shows 99% demultiplexing rate from raw data, 78% mapping rate, and 18% duplication rate of the 48 pooled samples and sequenced at 12 million reads per sample.
3. Mapping rates as high as 80% for different RNA inputs
Mapping rates are uniform across three RNA inputs tested: 1ug, 10ng, and 100ng. Human Universal Reference RNA was used to prepare mRNA Full-Length BRB libraries.
4. Full-Length BRB-seq exhibits reads distribution across the transcript’s entire length
5. >50'000 isoforms detected at 12M sequencing depth
Benchmarking the Full-Length BRB-seq protocol against competitor N shows similar performance for isoform detection. More than 50,000 isoforms can be detected at a sequencing depth of 12M reads per sample.
For (application) |
Full-Length mRNA sequencing |
For use with (equipment) |
Illumina and AVITI NGS instruments |
Species compatibility |
All eukaryotic species |
Available formats |
96 and 384 preps |
Shipping conditions |
Dry ice |
Storage conditions |
-20C |
Number of samples:
48
Reads per sample in demo dataset:
10'000 reads
To have access to the deep-sequenced dataset contact us.
Demo dataset file size:
9.9 MB
Each Full-Length DRUG-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different DRUG-seq pools.
For instance, the 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
The recommended range of RNA amount for each sample is 5’000 – 15’000 mammalian cells per well.
The minimum recommended RIN number is 7 and the A260/230 ratio (Nanodrop) should be in the 1.5-2.2 range.
The only difference between Full-Length DRUG-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the BRB barcode sequence.
For a thorough description of Full-Length DRUG-seq data processing, please look at the user guide.
The barcode set for your kit is conveniently located on the kit label. Please refer to the label for accurate identification.
For optimal compatibility, ensure that you use the appropriate plate format (e.g., for kits designed for 96 reactions, the 96 well-plate format should be used). This ensures accurate and efficient processing of your samples. If you have any further questions or concerns, please contact our support team for assistance by email or using our live chat tool.
Full-Length BRB-seq provides comprehensive coverage of the full-length mRNA transcripts.
We, therefore, normally recommend sequencing 12-20 million reads for each sample, which enables the reliable and unbiased detection of over 20,000 genes.
Our Full-Length DRUG-seq service delivers raw sequencing data (fastq files), gene count matrices, and analysis report files. A cost-efficient option suitable for projects of all sizes.
Book a one-on-one call with one of our RNA experts to discover how we can assist your next project.
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