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MERCURIUS™

High sensitivity BRB-seq service

The power of massively multiplexed RNA-seq, now compatible with low-input RNA samples, as low as 100pg.

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Benefits

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High sensitivity for low-input RNA

From as low as 100pg.

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Bulk RNA sequencing at scale

Massively multiplexed RNA-seq offers unparalleled cost and throughput scalability.

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Ideal for projects of all sizes

You can trust us. We have ran projects from as low as 6 samples to as many as 100'000.

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Fast turnaround times

From samples to data in as fast as 1.5 months.

What we do

MERCURIUS™

High sensitivity BRB-seq Service

The MERCURIUS™ High sensitivity BRB-seq service offers a convenient and streamlined solution for transcriptomics projects with a limited input amount of RNA (starting from 100pg).

Clients can send us purified RNA samples, which are always quality-checked before launching our BRB-seq pipeline. During the process, we always keep clients informed at the defined checkpoints so that we can decide together how to best proceed to the next steps.

Next-generation sequencing and data pre-processing (including alignment to the genome of choice) are also part of our standard service. As a result, we provide our clients raw data, sequencing and alignment reports, and gene count matrices which can be used for downstream gene expression analysis.

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How does it work

  • Client prepares the samples

    Sample preparation guidelines for low-input RNA
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  • Client sends submission form

    Sample submission form for MERCURIUSTM High sensitivity RNA BRB-seq
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  • Client ships samples to Alithea

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  • 1 week

    Incoming QC - Client Checkpoint

    (Nanodrop, Ribogreen, Tapestation)
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  • 2 days

    MERCURIUS™ High sensitivity BRB-seq library preparation for Illumina®

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  • 1 week

    Library QC - Client Checkpoint

    (Qubit, Fragment analyzer, shallow sequencing)
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  • 1.5 weeks

    Deep sequencing - Illumina

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  • 1 week

    Data analysis and reporting - Client Checkpoint

    Example of sample report
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  • Data

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  • Optional: Alithea performs Differential Gene Expression analysis​

FAQs

  • To guarantee high quality data, we normally request that each sample contains at least 200ng of total RNA in at least 10μl.
  • In addition to total RNA amount, it is important that the samples contain RNA of high integrity (RIN > 6) and are devoid of contaminants (Nanodrop A260/A230 between 1.8 and 2.2).
  • BRB-seq is 3’-end RNA sequencing method and, as such, requires significantly less sequencing as compared to standard full-length RNA-seq in order to reach accurate gene quantification. We therefore normally recommend to sequence 4 to 5 million reads for each sample, which enables the reliable and unbiased detection of over 18’000 genes.
  • As part of our standard service pipeline, we align the generated data to the genome of choice, provide a detailed report on the alignment and gene counting statistics and, finally, provide ready-to-use gene count matrices for downstream analysis.

See our products

Our BRB-seq and DRUG-seq v2 kits make it possible to process virtually any number of RNA samples in one single tube, which helps reduce technical variation, turnaround times and costs. Experience sample to sequencing-ready library in one day.

Discover our kits
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Speak with our RNA sequencing experts

Need guidance or have questions about BRB-seq and our service?

Easily book a call with our BRB-seq experts.