High Sensitivity BRB-seq service
The power of massively multiplexed RNA-seq, now compatible with low-input RNA samples, as low as 100pg per sample.
The MERCURIUS™ High sensitivity BRB-seq service offers a convenient and streamlined solution for transcriptomics projects with a limited input amount of RNA (starting from 100pg per sample).
Clients can send us purified RNA samples, which are always quality-checked before launching our BRB-seq pipeline. During the process, we always keep clients informed at the defined checkpoints so that we can decide together how to best proceed to the next steps.
Next-generation sequencing and data pre-processing (including alignment to the genome of choice) are also part of our standard service. As a result, we provide our clients raw data, sequencing and alignment reports, and gene count matrices which can be used for downstream gene expression analysis.
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(Nanodrop and Fragment Analyzer)
1 week
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(Qubit, Fragment analyzer, shallow sequencing)
1 week
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To guarantee high quality data, we normally request that each sample contains at least 200ng of total RNA in at least 10μl.
In addition to total RNA amount, it is important that the samples contain RNA of high integrity (RIN > 7) and are devoid of contaminants (Nanodrop A260/A230 between 1.8 and 2.2).
BRB-seq is 3’-end RNA sequencing method and, as such, requires significantly less sequencing as compared to standard full-length RNA-seq in order to reach accurate gene quantification. We therefore normally recommend to sequence 4 to 5 million reads for each sample, which enables the reliable and unbiased detection of over 18’000 genes.
As part of our standard service pipeline, we align the generated data to the genome of choice, provide a detailed report on the alignment and gene counting statistics and, finally, provide ready-to-use gene count matrices for downstream analysis.
Explore the latest, relevant publications in the industry to learn more about our technologies.
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