Benefits
High sensitivity for low-input RNA
From as low as 100pg per sample.
Bulk RNA sequencing at scale
Massively multiplexed RNA-seq offers unparalleled cost and throughput scalability.
Ideal for projects of all sizes
You can trust us. We have ran projects from as low as 6 samples to as many as 100'000.
Fast turnaround times
From samples to data in as little as one month.
What we do
MERCURIUS™
High sensitivity BRB-seq Service
The MERCURIUS™ High sensitivity BRB-seq service offers a convenient and streamlined solution for transcriptomics projects with a limited input amount of RNA (starting from 100pg per sample).
Clients can send us purified RNA samples, which are always quality-checked before launching our BRB-seq pipeline. During the process, we always keep clients informed at the defined checkpoints so that we can decide together how to best proceed to the next steps.
Next-generation sequencing and data pre-processing (including alignment to the genome of choice) are also part of our standard service. As a result, we provide our clients raw data, sequencing and alignment reports, and gene count matrices which can be used for downstream gene expression analysis.
How does it work
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Client ships samples to Alithea
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1 week
Incoming QC - Client Checkpoint
(Nanodrop, Ribogreen, Tapestation) -
2 days
MERCURIUS™ High sensitivity BRB-seq library preparation
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1 week
Library QC - Client Checkpoint
(Qubit, Fragment analyzer, shallow sequencing) -
1 week
Deep sequencing
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1 week
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Data
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Optional: Alithea performs Differential Gene Expression analysis
FAQs
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To guarantee high quality data, we normally request that each sample contains at least 200ng of total RNA in at least 10μl.
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In addition to total RNA amount, it is important that the samples contain RNA of high integrity (RIN > 6) and are devoid of contaminants (Nanodrop A260/A230 between 1.8 and 2.2).
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BRB-seq is 3’-end RNA sequencing method and, as such, requires significantly less sequencing as compared to standard full-length RNA-seq in order to reach accurate gene quantification. We therefore normally recommend to sequence 4 to 5 million reads for each sample, which enables the reliable and unbiased detection of over 18’000 genes.
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As part of our standard service pipeline, we align the generated data to the genome of choice, provide a detailed report on the alignment and gene counting statistics and, finally, provide ready-to-use gene count matrices for downstream analysis.
Dr. Nuno Miguel Luis, CNRS Researcher
Dr. Hirokazu Okada, ETH Zurich
Prof. Dr. Marc Robinson-Rechavi, University of Lausanne
Prof. Dr. Martin Klingenspor, TUM Munich
Speak with our RNA sequencing experts
Need guidance or have questions about BRB-seq and our service?
Easily book a call with our BRB-seq experts.