High sensitivity for low-input RNA
From as low as 100pg per sample.
Bulk RNA sequencing at scale
Massively multiplexed RNA-seq offers unparalleled cost and throughput scalability.
Ideal for projects of all sizes
You can trust us. We have ran projects from as low as 6 samples to as many as 100'000.
Fast turnaround times
From samples to data in as fast as 1.5 months.
What we do
High sensitivity BRB-seq Service
The MERCURIUS™ High sensitivity BRB-seq service offers a convenient and streamlined solution for transcriptomics projects with a limited input amount of RNA (starting from 100pg per sample).
Clients can send us purified RNA samples, which are always quality-checked before launching our BRB-seq pipeline. During the process, we always keep clients informed at the defined checkpoints so that we can decide together how to best proceed to the next steps.
Next-generation sequencing and data pre-processing (including alignment to the genome of choice) are also part of our standard service. As a result, we provide our clients raw data, sequencing and alignment reports, and gene count matrices which can be used for downstream gene expression analysis.
How does it work
Client ships samples to Alithea
Incoming QC - Client Checkpoint(Nanodrop, Ribogreen, Tapestation)
MERCURIUS™ High sensitivity BRB-seq library preparation for Illumina®
Library QC - Client Checkpoint(Qubit, Fragment analyzer, shallow sequencing)
Deep sequencing - Illumina
Optional: Alithea performs Differential Gene Expression analysis
To guarantee high quality data, we normally request that each sample contains at least 200ng of total RNA in at least 10μl.
In addition to total RNA amount, it is important that the samples contain RNA of high integrity (RIN > 6) and are devoid of contaminants (Nanodrop A260/A230 between 1.8 and 2.2).
BRB-seq is 3’-end RNA sequencing method and, as such, requires significantly less sequencing as compared to standard full-length RNA-seq in order to reach accurate gene quantification. We therefore normally recommend to sequence 4 to 5 million reads for each sample, which enables the reliable and unbiased detection of over 18’000 genes.
As part of our standard service pipeline, we align the generated data to the genome of choice, provide a detailed report on the alignment and gene counting statistics and, finally, provide ready-to-use gene count matrices for downstream analysis.
Dr. Nuno Miguel Luis, CNRS Researcher
Dr. Hirokazu Okada, ETH Zurich
Prof. Dr. Marc Robinson-Rechavi, University of Lausanne
Prof. Dr. Martin Klingenspor, TUM Munich
Speak with our RNA sequencing experts
Need guidance or have questions about BRB-seq and our service?
Easily book a call with our BRB-seq experts.