FFPE-seq Services | RNA-seq for heavily degraded samples

Benefits

Ideal for degraded RNA samples: Compatible with FFPE RNA and samples with RIN values as low as 1.

Bulk RNA sequencing at scale: Unparalleled cost and throughput scalability.

Fast turnaround times: From samples to data in as little as one month.

MERCURIUS^TM

FFPE-seq service

The MERCURIUS™ FFPE-seq service is designed to cope with degraded RNA samples, such as FFPE samples, offering a convenient and streamlined solution for transcriptomics projects. Clients can send us directly purified RNA samples. During the process, we always keep clients informed at defined checkpoints so that we can decide together how to best proceed to the next steps.

Next generation sequencing and data pre-processing (including alignment to the genome of choice) are part of our standard service as well. As a result, we provide our clients raw data, sequencing and alignment reports, and gene count matrices which can be used for downstream gene expression analysis.

How it works

Step 1

Client prepares the samples

Sample preparation guidelines for FFPE-seq

Step 2

Client sends submission form

Sample submission form for MERCURIUS^TMFFPE-seq service

Step 3

Client ships samples to Alithea

Step 4

MERCURIUS™ FFPE-seq library preparation

2 days

Step 5

Library QC - Client Checkpoint

(Qubit, Fragment analyzer, shallow sequencing)

1 week

Step 6

Deep sequencing

1 week

Step 7

Data analysis and reporting - Client Checkpoint

Example of sample report

1 week

Step 8

Data

Optional: Alithea performs Differential Gene Expression analysis

Performance

Read more about the FFPE-seq on our blog.

FFPE-seq provides full-length gene coverage

Genome browser view of the RNA-seq reads mapped to several human genes, using different library preparation protocols. The FFPE-seq shows comparable coverage as the NEB Ultra II, while the BRB-seq protocol is limited only to the 3' end genomic region.

Gene body coverage of FFPE-seq

Gene body coverage from FFPE-seq, BRB-seq, and NEB Ultra II. The FFPE-seq shows a similar coverage profile as the NEB Ultra II. By contrast, BRB-seq reads preferentially mapped to the 3'end.

FAQs

Relevant publications

Explore the latest, relevant publications in the industry to learn more about our technologies.

Explore

Cells and organoids (Extraction-free)

Purified RNA

Accessories

Cells and Organoids (EXTRACTION-FREE)

PURIFIED RNA

Whole Blood RNA

Cells and organoids (Extraction-free)

Purified RNA

Accessories

Cells and Organoids (EXTRACTION-FREE)

PURIFIED RNA

Whole Blood RNA

FFPE-seq service

Benefits

Experimental workflow at a glance

FFPE-seq service

How it works

Client prepares the samples

Client sends submission form

Client ships samples to Alithea

MERCURIUS™ FFPE-seq library preparation

Library QC - Client Checkpoint

Deep sequencing

Data analysis and reporting - Client Checkpoint

Data

Optional: Alithea performs Differential Gene Expression analysis

Performance

FFPE-seq provides full-length gene coverage

Gene body coverage of FFPE-seq

FAQs

Relevant publications

Speak with one of our RNA experts

Subscribe to our newsletter

Get in touch

FFPE-seq service

Benefits

Experimental workflow at a glance

FFPE-seq service

How it works

Client prepares the samples

Client sends submission form

Client ships samples to Alithea

MERCURIUS™ FFPE-seq library preparation

Library QC - Client Checkpoint

Deep sequencing

Data analysis and reporting - Client Checkpoint

Data

Optional: Alithea performs Differential Gene Expression analysis​

Performance

FFPE-seq provides full-length gene coverage

Gene body coverage of FFPE-seq

FAQs

Relevant publications

Speak with one of our RNA experts

Subscribe to our newsletter

Optional: Alithea performs Differential Gene Expression analysis