MERCURIUS™

Targeted Plant BRB-seq kits

Massively multiplexed and targeted RNA-seq from purified plant RNA samples - compatible with all eukaryotic species.

Compatible with Illumina^®and AVITI™ sequencing systems.

Benefits

The MERCURIUS™ Targeted Plant BRB-seq kits  offer the most comprehensive, high-throughput, and cost-efficient solution for targeted gene expression analysis projects. The kits contain all the oligos and enzymes needed to go from purified RNA to sequencing-ready libraries.

Custom-developed panels: Users communicate their targets of interest, and we will develop ad-hoc detection panels.

Up to 100 targets: Experimentally validated panels for differential expression analysis.

Bulk RNA sequencing at scale: Perform up-to 384 RNA-seq library preparations in one single tube.

One-day lab workflow: Convenient and short protocol from samples to sequencing-ready libraries in one day.

MERCURIUS^TM

Targeted

Plant BRB-seq kits

The production of our Targeted Plant BRB-seq kits starts with the client providing us with their genes of interest. We then develop a corresponding custom detection panel and validate it directly using their RNA samples.

During the development process, the client provides us with purified plant RNA samples for gene panel validation.

Once the targeted gene panel validation is complete, we will notify the client and ship a pilot batch of Targeted Plant BRB-seq library prep kits. Upon receiving the client's approval, we will commence production of the custom kit batch.

How it works

Step 1

Client communicates the genes of interest panel

Gene panel submission form for Targeted BRB-seq or Targeted DRUG-seq

Step 2

Client ships representative purified RNA samples to Alithea for panel validation

Sample submission guidelines for MERCURIUS^TM Targeted BRB-seq kits

Step 3

Alithea develops and validates the custom detection panel on customer's samples

4 to 8 weeks

Step 4

Client checkpoint - Alithea ships a pilot batch

Step 5

Upon client's approval, Alithea begins the dedicated batch production of custom kits

Performance
Product specifications

1. Improvement of target gene detection with Targeted BRB-seq

Gene ranked by detection abundance with MERCURIUS™ Targeted BRB-seq on a panel of 24 target genes compared to MERCURIUS™ BRB-seq.

For (application)	Targeted RNA sequencing
For use with (equipment)	Illumina® , AVITI™ and MGI instruments
Species compatibility	All eukaryotic species
Shipping conditions	Dry ice
Storage conditions	-20C

1. Improvement of target gene detection with Targeted BRB-seq

Gene ranked by detection abundance with MERCURIUS™ Targeted BRB-seq on a panel of 24 target genes compared to MERCURIUS™ BRB-seq.

Product specifications

FAQs

Each Targeted BRB-seq contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient to complete the library preparation process for four different BRB-seq pools.

To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-sample kit can be used to prepare up to 96 samples distributed across up to four different libraries.

Try our service

The MERCURIUS™ Targeted Plant BRB-seq service offers the most comprehensive, high-throughput and cost-efficient solution for targeted gene expression analysis projects starting from purified RNA.

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