MERCURIUS™
Total DRUG-seq kits
Ultra-scalable, extraction-free total RNA-seq for transcriptomic profiling and target discovery
Compatible with Illumina®, AVITI™ and MGI*.
* For native kits' compatibility with MGI sequencers, contact us.
Catalog number | #10705 |
#11661 |
#10706 |
#11662 |
Total preps
|
96 | 384 | 384 | 1'536 |
Sample multiplexing and plate format
|
96 | 96 | 384 | 384 |
Barcoded oligo-dT plates included
|
1 | 4 | 1 | 4 |
UDI pairs included
|
4 | 4 | 4 | 4 |
Documentation |
Cat ##10705
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||||
---|---|---|---|---|
Total reactions
|
96 | |||
RNA multiplexing format
|
96 | |||
UDI pairs included
|
4 | |||
Cat ##11661
![]() |
||||
---|---|---|---|---|
Total reactions
|
384 | |||
RNA multiplexing format
|
96 | |||
UDI pairs included
|
4 | |||
Cat ##10706
![]() |
||||
---|---|---|---|---|
Total reactions
|
384 | |||
RNA multiplexing format
|
384 | |||
UDI pairs included
|
4 | |||
Cat ##11662
![]() |
||||
---|---|---|---|---|
Total reactions
|
||||
RNA multiplexing format
|
384 | |||
UDI pairs included
|
4 | |||
- Products
- Cells and Organoids (Extraction-free)
- DRUG-seq kits
Benefits
- Ideal for transcriptomic profiling and target validation
- Robust results empower faster, data-rich decision-making in the drug discovery pipeline.
- Multiplexed full-length RNA
- Full-length RNA-seq library prep, 96 or 384 samples in a single tube. For both coding and non-coding RNAs.
- Full-length transcript coverage
From differential gene expression to transcript variants, alternative splicing, or fusion genes.
- No prior RNA extraction
- An optimized lysis buffer for complete lysis and efficient reverse transcription.
- Improved protocol
Without pre-amplification, leading to higher mapping and gene detection rates.
- One-day lab workflow
- Convenient and short protocol from samples to sequencing-ready libraries in one day.
- Experimental workflow at a glance
- Performance
- Product specifications
- Validated cell lines
1. Total DRUG-seq has a high demultiplexing rate and the majority of reads map to exons or the genome
Total DRUG-seq library performance sequenced at 5.6 million reads per sample.
2. Total DRUG-seq exhibits reads distribution across the transcript’s entire length
The gene body coverage shows a consistent and uniform reads distribution across the entire gene body for the Total DRUG-seq protocol, comparable to the competitor N protocol, while the 3' DRUG-seq protocol shows a significant 3' bias due to its poly-A selection methodology.
3. Total DRUG-seq exhibits an improved protocol for transcript detection
The MERCURIUS™ Total DRUG-seq features an improved protocol that enables more comprehensive transcript detection compared to the 3' DRUG-seq approach, delivering deeper insights for compound screening and target validation.
4. Identification of alternative promoter usage with Total DRUG-seq
MERCURIUS™ Total DRUG-seq reveals differential promoter usage in response to TGFB treatment: HNF4A promoter 1 (P1)is inhibited, while promoter 2 (P2) is activated. This switch in promoter activity is a hallmark of epithelial-to-mesenchymal transition and is associated with tumor progression. Activation of HNF4A P2 is a known marker of hepatocarcinoma progression, highlighting the power of Total DRUG-seq to resolve complex transcriptional regulation at the promoter level.
For (application) |
Full-length RNA sequencing |
For use with (equipment) |
Illumina and AVITI or MGI instruments |
Species compatibility |
All eukaryotic species |
Available formats |
96 and 384 preps |
Shipping conditions |
Dry ice |
Storage conditions |
-20C |
Cell line/Tissue | Organism |
HT1080 SMARCA4 KO | Human |
hTEC | Human |
iPSC microglia | Human |
MCF7 | Human |
HepG2_IGF1RKO | Human |
Beas-2B | Human |
dTHP-1 | Human |
iPSC derived cardiomyocytes | Human |
Endothelial | Human |
Hepatocyte | Human |
HEPG2 | Human |
Hepatocyte | Human |
SF9 | Spodoptera |
A549 | Human |
Breast Cancer (MCF7) | Human |
B lymphoblast MM.1s. | Human |
Hek293 | Human |
HeLa Cells | Human |
U2-OS | Human |
Hek293 | Human |
dTHP-1 | Human |
AsPC-1 | Human |
PBMC (TCD4) | Human |
Skeletal muscle (LHCN-M2) | Human |
HepaRG | Human |
Macrophage (MV-4-11) | Human |
PBMCs (Blood) | Human |
Microglia | Human |
Fibroblast Like Synoviocyte donor 1502 | Human |
Fibroblast | Human |
iPSC-derived neuron | Human |
Human lung carcinoma epithelial cell line | Human |
Human breast epithelial adenocarcinoma cell line | Human |
Human liver hepatocellular carcinoma cell line | Human |
Human cardiomyocytes derived from induced pluripotent stem cells (iPSCs) | Human |
-
-
1. Total DRUG-seq has a high demultiplexing rate and the majority of reads map to exons or the genome
Total DRUG-seq library performance sequenced at 5.6 million reads per sample.
2. Total DRUG-seq exhibits reads distribution across the transcript’s entire length
The gene body coverage shows a consistent and uniform reads distribution across the entire gene body for the Total DRUG-seq protocol, comparable to the competitor N protocol, while the 3' DRUG-seq protocol shows a significant 3' bias due to its poly-A selection methodology.
3. Total DRUG-seq exhibits an improved protocol for transcript detection
The MERCURIUS™ Total DRUG-seq features an improved protocol that enables more comprehensive transcript detection compared to the 3' DRUG-seq approach, delivering deeper insights for compound screening and target validation.
4. Identification of alternative promoter usage with Total DRUG-seq
MERCURIUS™ Total DRUG-seq reveals differential promoter usage in response to TGFB treatment: HNF4A promoter 1 (P1)is inhibited, while promoter 2 (P2) is activated. This switch in promoter activity is a hallmark of epithelial-to-mesenchymal transition and is associated with tumor progression. Activation of HNF4A P2 is a known marker of hepatocarcinoma progression, highlighting the power of Total DRUG-seq to resolve complex transcriptional regulation at the promoter level.
-
For (application)
Full-length RNA sequencing
For use with (equipment)
Illumina and AVITI or MGI instruments
Species compatibility
All eukaryotic species
Available formats
96 and 384 preps
Shipping conditions
Dry ice
Storage conditions
-20C
-
Cell line/Tissue Organism HT1080 SMARCA4 KO Human hTEC Human iPSC microglia Human MCF7 Human HepG2_IGF1RKO Human Beas-2B Human dTHP-1 Human iPSC derived cardiomyocytes Human Endothelial Human Hepatocyte Human HEPG2 Human Hepatocyte Human SF9 Spodoptera A549 Human Breast Cancer (MCF7) Human B lymphoblast MM.1s. Human Hek293 Human HeLa Cells Human U2-OS Human Hek293 Human dTHP-1 Human AsPC-1 Human PBMC (TCD4) Human Skeletal muscle (LHCN-M2) Human HepaRG Human Macrophage (MV-4-11) Human PBMCs (Blood) Human Microglia Human Fibroblast Like Synoviocyte donor 1502 Human Fibroblast Human iPSC-derived neuron Human Human lung carcinoma epithelial cell line Human Human breast epithelial adenocarcinoma cell line Human Human liver hepatocellular carcinoma cell line Human Human cardiomyocytes derived from induced pluripotent stem cells (iPSCs) Human
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