MERCURIUS™
Multiplexed Total RNA-seq
Full-length and total RNA-seq combined with massive sample multiplexing.
The MERCURIUS™ Multiplexed Total RNA-seq service offers a convenient and streamlined solution for full-length total RNA transcriptomics projects of any size.
Clients can send us purified RNA samples, which are always quality-checked before launching our Multiplexed Total RNA-seq pipeline. During the process, we always keep clients informed at defined checkpoints so that we can decide together how to best proceed to the next steps.
Next generation sequencing and data pre-processing (including alignment to the genome of choice) are part of our standard service as well. As a result, we provide our clients raw data, sequencing and alignment reports, and gene count matrices which can be used for downstream gene expression analysis.
Optional downstream differential gene expression analysis are also available upon request.


Step 1
Step 2
Step 3
Step 4
(Nanodrop and Fragment Analyzer)
1 week
Step 5
2 days
Step 6
(Qubit, Fragment analyzer, shallow sequencing)
1 week
Step 7
1 week
Step 8
Step 9
To generate high-quality sequencing data, we recommend between 100pg to 100ng total purified RNA per sample.
In addition to total RNA amount, it is important that the samples contain RNA of high integrity (RIN > 7) and are devoid of contaminants (Nanodrop A260/A230 > 1.5).
Multiplexed Total RNA-seq provides comprehensive coverage of the full-length RNA transcripts.
We, therefore, normally recommend sequencing 12-20 million reads for each sample, which enables the reliable and unbiased detection of over 20,000 genes.
Explore the latest, relevant publications in the industry to learn more about BRB-seq.
Book a one-on-one call with one of our RNA experts to discover how we can assist your next project.
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