MERCURIUS™
Total Blood BRB-seq service
By leveraging BRB-seq, we not only provide to industrial and academic clients high quality RNA-seq data, but we also do so with the highest affordability and shortest turnaround times on the market.
- Bulk RNA sequencing at scale
- Unparalleled cost and throughput scalability.
- Multiplexed total RNA-seq
- 96 samples multiplexed in a single tube.
- Inline globin depletion
- Seamlessly integrated globin depletion.
- Full-length transcript coverage
- From differential gene expression to splicing variants and isoform detection.
- Fast turnaround times
- From samples to data in as little as six weeks.
MERCURIUSTM
Total Blood BRB-Seq service
The MERCURIUS™ Total Blood BRB-seq service offers a convenient and streamlined solution for human blood transcriptomics projects. We have expertise with different types of RNA purification from blood samples and we have specialised in high-throughput RNA extraction from PaxGene tubes.
Customers can therefore either send us directly their human blood samples or perform RNA extraction in-house and send us purified RNA samples. Upon sample reception, we always perform incoming QC before launching our Total Blood BRB-seq pipeline. During the process, we always keep clients informed at defined checkpoint so that we can decide together how to best proceed to the next steps.
Next-generation sequencing and data preprocessing (including alignment to the genome) are part of our standard service as well. As a result, we provide our clients raw data, sequencing and alignment reports, and gene count matrices which can be used for downstream gene expression analysis.
How it works
Step 1
Client ships samples to Alithea
For human blood samples, we offer high-throughput RNA extraction directly from PAXgene®, Tempus™ Blood RNA and RNA shield tubes.
Step 2
Incoming QC - Client Checkpoint
(Nanodrop, Fragment Analyzer)
1 week
Step 3
MERCURIUS™ Total Blood BRB-seq library preparation
2 days
Step 4
Library QC - Client Checkpoint
(Nanodrop and Fragment Analyzer)
1 week
Step 5
Deep sequencing
1 week
Step 6
Step 7
Data
FAQs
Relevant publications
Explore the latest, relevant publications in the industry to learn more about our technologies.
Want to do Total Blood BRB-seq in-house?
Our Total Blood BRB-seq kits make it possible to process virtually any number of RNA samples in one single tube, which helps reduce technical variation, turnaround times and costs.
Experience sample to sequencing-ready library in one day.
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