MERCURIUS™

Total Blood BRB-seq kits

Full-length and total RNA library preparation kits featuring early multiplexing for purified blood RNA - compatible with human samples

RNA-seq library preps with integrated globin depletion.

Compatible with Illumina® and AVITI™ sequencers.


Catalog number	#10720	#11720
Total preps How many library preps can be prepared in total with one kit	96	384
Sample multiplexing and plate format How many samples can be multiplexed in one single tube	96	96
Barcoded oligo-dT plates included	1	4
UDI pairs included How many individual libraries can be sequenced together	4	4
Globin blockers	included	included
Documentation	User guide Barcode files

Cat ##10720
Total reactions How many library preps can be prepared in total with one kit	96
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4
Globin blockers	included
Download Manual

Cat ##11720
Total reactions How many library preps can be prepared in total with one kit	384
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4
Globin blockers	included
Download Manual

Benefits

The  MERCURIUS™ Total Blood BRB-seq library preparation kits for Illumina^®and AVITI™ contain all the oligos and enzymes needed to go from purified blood RNA to sequencing-ready libraries.

Multiplexed full-length RNA: Full-length RNA-seq library prep, 96 samples in a single tube. For both coding and non-coding RNAs.

Full-length transcript coverage: From differential gene expression to transcript variants, alternative splicing, or fusion genes.

Inline globin depletion: Seamlessly integrated globin depletion. No need to purchase additional kits.

One-day lab workflow: Convenient and short protocol from samples to sequencing-ready libraries in one day.

Experimental workflow at a glance
Performance
Product specifications

Total Blood BRB-seq-3

1. Detection of 17'000+ genes per sample across 96 samples

Distribution of the number of detected genes across 96 samples prepared with the MERCURIUS™ Blood BRB-seq library preparation kit and the MERCURIUS™ Total Blood BRB-seq. The libraries were sequenced at the same sequencing depth.

2. Total Blood BRB-seq provides effective and high-throughput inline depletion of globin genes

With and without GB_ Total Blood BRB-seq@500x

(Top) The distribution of reads mapped to non-globin and globin genes per sample for one 96 sample Total Blood BRB-seq library without globin depletion. An average of approximately one-third of reads mapped to globin genes. (Bottom) The distribution of reads mapped to non-globin and globin genes per sample for the same 96 samples in one Total Blood BRB-seq library treated with globin depletion. The reads associated with globin genes are drastically reduced, with more reads mapped to non-globin genes.

3. Gene saturation analysis comparing the Blood BRB-seq and the Total Blood BRB-seq library preparation workflows

The plot shows the number of detected genes as a function of sequencing depth for both MERCURIUS™ Blood BRB-seq library preparation kit and the MERCURIUS™ Total Blood BRB-seq.

4. Total Blood BRB-seq exhibits reads distribution across the transcript’s entire length

The gene body coverage shows a consistent and uniform read distribution across the entire gene body for the Total Blood BRB-seq protocol, while the Blood BRB-seq protocol shows a significant 3' bias due to its poly-A selection methodology.

For (application)	Total RNA sequencing
For use with (equipment)	Illumina and AVITI NGS instruments
Species compatibility	Human
Available formats	96 preps
Shipping conditions	Dry ice
Storage conditions	-20C

1. Detection of 17'000+ genes per sample across 96 samples

Distribution of the number of detected genes across 96 samples prepared with the MERCURIUS™ Blood BRB-seq library preparation kit and the MERCURIUS™ Total Blood BRB-seq. The libraries were sequenced at the same sequencing depth.

2. Total Blood BRB-seq provides effective and high-throughput inline depletion of globin genes

(Top) The distribution of reads mapped to non-globin and globin genes per sample for one 96 sample Total Blood BRB-seq library without globin depletion. An average of approximately one-third of reads mapped to globin genes. (Bottom) The distribution of reads mapped to non-globin and globin genes per sample for the same 96 samples in one Total Blood BRB-seq library treated with globin depletion. The reads associated with globin genes are drastically reduced, with more reads mapped to non-globin genes.

3. Gene saturation analysis comparing the Blood BRB-seq and the Total Blood BRB-seq library preparation workflows

The plot shows the number of detected genes as a function of sequencing depth for both MERCURIUS™ Blood BRB-seq library preparation kit and the MERCURIUS™ Total Blood BRB-seq.

4. Total Blood BRB-seq exhibits reads distribution across the transcript’s entire length

The gene body coverage shows a consistent and uniform read distribution across the entire gene body for the Total Blood BRB-seq protocol, while the Blood BRB-seq protocol shows a significant 3' bias due to its poly-A selection methodology.

Product specifications

FAQs

Each Total Blood BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.

To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-sample kit can be used to prepare up to 96 samples distributed across up-to four different libraries.
The barcode set for your kit is conveniently located on the kit label. Please refer to the label for accurate identification.

For optimal compatibility, ensure that you use the appropriate plate format (e.g., for kits designed for 96 reactions, the 96 well-plate format should be used). This ensures accurate and efficient processing of your samples. If you have any further questions or concerns, please contact our support team for assistance by email or using our live chat tool.

Relevant publications

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Let us do it for you. Our team delivers raw sequencing data (fastq files), gene count matrices and analysis report files. A cost-efficient option suitable for projects of all sizes.

DISCOVER MORE

CELLS & ORGANOIDS (Extraction-free)

PURIFIED RNA

PLANT RNA

BLOOD RNA

Accessories

CELLS (EXTRACTION-FREE)

ORGANOIDS (EXTRACTION-FREE)

PURIFIED RNA

PLANT RNA

BLOOD RNA

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DRUG-seq

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FLASH-seq

MERCURIUS™
BRB-seq

CELLS & ORGANOIDS (Extraction-free)

PURIFIED RNA

PLANT RNA

BLOOD RNA

Accessories

CELLS (EXTRACTION-FREE)

ORGANOIDS (EXTRACTION-FREE)

PURIFIED RNA

PLANT RNA

BLOOD RNA

MERCURIUS™
DRUG-seq

MERCURIUS™
FLASH-seq

MERCURIUS™
BRB-seq

Total Blood BRB-seq kits

Accessories

UDI X-Leap module

Benefits

FAQs

Relevant publications

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