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MERCURIUS™

DRUG-seq
Extraction-free library preparation kits for Illumina®

RNA-seq library preps directly from cell lysates without prior RNA isolation.

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Cat #10841 CP_AG_FrontTall_Comp_Transparent_Small
Total reactions How many library preps can be prepared in total with one kit 96
RNA multiplexing format How many library preps can be prepared in total with one kit 96
UDI pairs included How many library preps can be prepared in total with one kit 4
Cat #11041 CP_AG_FrontTall_Comp__Small
Total reactions How many library preps can be prepared in total with one kit 384
RNA multiplexing format How many library preps can be prepared in total with one kit 96
UDI pairs included How many library preps can be prepared in total with one kit 4
Cat #10851 CP_AG_FrontTall_Comp_Transparent_Small
Total reactions How many library preps can be prepared in total with one kit 384
RNA multiplexing format How many library preps can be prepared in total with one kit 384
UDI pairs included How many library preps can be prepared in total with one kit 4
Cat #11051 CP_AG_FrontTall_Comp__Small
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit 384
UDI pairs included How many library preps can be prepared in total with one kit 4
4x Cat #11051 CP_AG_FrontTall_Comp__Small
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit 384
UDI pairs included How many library preps can be prepared in total with one kit 16
16x Cat #11051 CP_AG_FrontTall_Comp__Small
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit 384
UDI pairs included How many library preps can be prepared in total with one kit 16

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Custom Format

For more reactions, higher multiplexing and more UDIs

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Benefits

The MERCURIUS™ Extraction-free DRUG-seq kits contain all the oligos and enzymes needed to go from 2D cell cultures to sequencing-ready DNA libraries.

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Ideal for screening projects

More samples, more replicates. Robust results, significant discoveries.

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No need for prior RNA extraction

An optimized lysis buffer for complete lysis and efficient reverse transcription.

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Improved DRUG-seq protocol

Without pre-amplification, leading to higher mapping and gene detection rates.

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One-day lab workflow

Convenient and short protocol from samples to sequencing-ready libraries in one day.

Experimental workflow at a glance

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Performance

Product Specifications

For (application)
3' mRNA sequencing
For use with (equipment)
Illumina NGS instruments
Species compatibility
All eukaryotic species
Available formats
96, 384, 1'536, 6'144, 24'576 reactions
Shipping conditions
Dry ice
Storage conditions
-20C

Sample datasets

  • Cell line-1

    Hap1

    Number of samples:
    24
     
    Reads per sample in demo dataset:
    10'000 reads
     
    To have access to the deep-sequenced dataset (7.3 M reads per sample) contact us.
     
    Demo dataset file size:
    13.3 MB
    Download
    Cell line-1

    HepG2

    Number of samples:
    65
     
    Reads per sample in demo dataset:
    10'000 reads
     
    To have access to the deep-sequenced dataset (3.9 M reads per sample) contact us.
     
    Demo dataset file size:
    4.76 MB
    Download
    Cell line-1

    HeLa

    Number of samples:
    24
     
    Reads per sample in demo dataset:
    10'000 reads
     
    To have access to the deep-sequenced dataset (7.1 M reads per sample) contact us.
     
    Demo dataset file size:
    13.6 MB
    Download

FAQs

  • Each BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.

    To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.

  • The recommended range of input material is in the range of 5’000-50’000 cells.
  • The only difference between DRUG-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the DRUG-seq barcode sequence.

    For a thorough description of DRUG-seq data processing, please refer to the DRUG-seq kit user guide. 

Try our services

Our BRB-seq and DRUG-seq services deliver raw sequencing data (fastq files), gene count matrices and analysis report files. A cost-efficient option suitable for projects of all sizes.

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