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MERCURIUS™

DRUG-seq kits

Multiplexed extraction-free library preparation kits

for Illumina®, AVITI™ and MGI*

RNA-seq library preps directly from cell lysates without prior RNA isolation.

* For native kits' compatibility with MGI sequencers, contact us.

Cat ##10841
Total reactions How many library preps can be prepared in total with one kit 96
RNA multiplexing format How many library preps can be prepared in total with one kit 96
UDI pairs included How many library preps can be prepared in total with one kit 4
Download Manual
Cat ##11041
Total reactions How many library preps can be prepared in total with one kit 384
RNA multiplexing format How many library preps can be prepared in total with one kit 96
UDI pairs included How many library preps can be prepared in total with one kit 4
Download Manual
Cat ##10851
Total reactions How many library preps can be prepared in total with one kit 384
RNA multiplexing format How many library preps can be prepared in total with one kit 384
UDI pairs included How many library preps can be prepared in total with one kit 4
Download Manual
Cat ##11051
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit 384
UDI pairs included How many library preps can be prepared in total with one kit 4
Download Manual

Add-ons

Accessories

Cell lysis
module

 

 

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  • Optimized lysis buffer for complete lysis and efficient reverse transcription.
  • Compatible with DRUG-seq library prep kits. 

Find out more

Accessories

UDI expansion
module

 

 

Asset 1-Jun-21-2023-09-58-09-8501-AM

 

 

  • Compatible with the MERCURIUS™ product line.
  • 12 UDI pairs.   
  • Flexible dual indexing solutions. 

Find out more

  • Benefits

    The MERCURIUS™ Extraction-free DRUG-seq kits contain all the oligos and enzymes needed to go from 2D cell cultures to sequencing-ready libraries.

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    		 benefits_NoRNAExt_gradient

     

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    Ideal for screening projects No need for prior RNA extraction Improved DRUG-seq protocol

    One-day lab workflow

    More samples, more replicates. Robust results, significant discoveries.

     An optimized lysis buffer for complete lysis and efficient reverse transcription.

    Without pre-amplification, leading to higher mapping and gene detection rates.

    Convenient and short protocol from samples to sequencing-ready libraries in one day.

     

  • Experimental workflow at a glance

    DRUG-seq-2-1

     

  • Performance

    1. Detection of 12'000+ genes at 1.5 million reads per sample across 384 samples

     

    Number of detected genes@300x

    Distribution of the number of detected genes across 384 samples prepared with the MERCURIUS™ DRUG-seq library preparation kit from frozen cells. The library was sequenced at an average of 1.5 million reads per sample on an Illumina NovaSeq 6000.

     

    2. DRUG-seq shows high demultiplexing rate and high mapping rate to exons

     

    Mapping and alignment @300x-1

    DRUG-seq performance shows 97% demultiplexing rate from raw data and 85% mapping rate of the 384 pooled samples, sequenced at 1.5 million reads per sample.

  • Product specifications

     

    For (application)

    3' mRNA sequencing

    For use with (equipment)

    Illumina and AVITI NGS instruments

    Species compatibility

    All eukaryotic species

    Available formats

    96, 384, 1'536, 6'144 and 24'576 preps

    Shipping conditions

    Dry ice

    Storage conditions

    -20C

     

  • Validated cell lines

      Cell line Specie Tissue Culture type
      A375 h.sapiens Skin, malignant melanoma adherent
      GM12878 h.sapiens PBMC, lymphoblastoid suspension
      H295R h.sapiens Adrenal gland, carcinoma adherent
      HAP1 h.sapiens KBM-7 derived, chronic
    myelogenous leukemia    		 adherent
      hASC h.sapiens Patient-derived adipose stromal cells adherent
      hASC-Adipocytes h.sapiens Differentiated hASC adherent
      HEK293 h.sapiens Kidney embryonic  adherent
      HeLa h.sapiens Cervix, adenocarcinoma adherent
      HepG2 h.sapiens Liver, carcinoma adherent
      Huh7 h.sapiens Liver, carcinoma adherent
      iNeurons h.sapiens Differentiated iPSC adherent
      MCF-7 h.sapiens Breast, adenocarcinoma adherent
      PANC-1 h.sapiens Pancreas, carcinoma adherent
      U2OS h.sapiens Bone, osteosarcoma adherent

Sample datasets

  • Cell line-1

    Hap1

    Number of samples:
    24
     
    Reads per sample in demo dataset:
    10'000 reads
     
    To have access to the deep-sequenced dataset (7.3 M reads per sample) contact us.
     
    Demo dataset file size:
    13.3 MB
    Download
    Cell line-1

    HepG2

    Number of samples:
    65
     
    Reads per sample in demo dataset:
    10'000 reads
     
    To have access to the deep-sequenced dataset (3.9 M reads per sample) contact us.
     
    Demo dataset file size:
    4.76 MB
    Download
    Cell line-1

    HeLa

    Number of samples:
    24
     
    Reads per sample in demo dataset:
    10'000 reads
     
    To have access to the deep-sequenced dataset (7.1 M reads per sample) contact us.
     
    Demo dataset file size:
    13.6 MB
    Download

FAQs

  • Each DRUG-seq or BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.

    To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.

  • The recommended range of input material is in the range of 5’000-50’000 cells.

  • The only difference between DRUG-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the DRUG-seq barcode sequence.

    For a thorough description of DRUG-seq data processing, please refer to the DRUG-seq kit user guide. 

  • The barcode set for your kit is conveniently located on the kit label. Please refer to the label for accurate identification.

    For optimal compatibility, ensure that you use the appropriate plate format (e.g., for kits designed for 96 reactions, the 96 well-plate format should be used). This ensures accurate and efficient processing of your samples. If you have any further questions or concerns, please contact our support team for assistance by email or using our live chat tool.

Try our services

Our BRB-seq and DRUG-seq services deliver raw sequencing data (fastq files), gene count matrices and analysis report files. A cost-efficient option suitable for projects of all sizes.

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