MERCURIUS™ DRUG-Seq-kits | High-throughput low-cost RNA seq

	Cat #10841	Cat #11041	Cat #10851	Cat #11051	4x Cat #11051	16x Cat #11051
Total reactions How many library preps can be prepared in total with one kit	96	384	384	1'536	6'144	36'864
RNA multiplexing format How many samples can be multiplexed in one single tube	96	96	384	384	384	384
UDI pairs included How many individual BRB-seq libraries can be pooled and sequenced together	4	4	4	4	4	4
Data analysis
Manual	Download Manual

Cat #10841
Total reactions How many library preps can be prepared in total with one kit	96
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4

Download Manual

Cat #11041
Total reactions How many library preps can be prepared in total with one kit	384
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4

Download Manual

Cat #10851
Total reactions How many library preps can be prepared in total with one kit	384
RNA multiplexing format How many library preps can be prepared in total with one kit	384
UDI pairs included How many library preps can be prepared in total with one kit	4

Download Manual

Cat #11051
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit	384
UDI pairs included How many library preps can be prepared in total with one kit	4

Download Manual

4x Cat #11051
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit	384
UDI pairs included How many library preps can be prepared in total with one kit	4

Download Manual

16x Cat #11051
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit	384
UDI pairs included How many library preps can be prepared in total with one kit	4

Download Manual

More samples, more replicates. Robust results, significant discoveries.

Demultiplex and align your DRUG-seq data with our easy-to-use cloud-based platform.

An optimized lysis buffer for complete lysis and efficient reverse transcription.

Without pre-amplification, leading to higher mapping and gene detection rates.

Convenient and short protocol from samples to sequencing-ready libraries in one day.

Performance

Distribution of the number of detected genes across...

For use with (application)

3' mRNA sequencing

For use with (equipment)

Illumina NGS instruments

Species compatibility

All eukaryotic species

Available formats

96, 384, 1'536, 6'144, 36'864 reactions

Shipping conditions

Dry ice

Storage conditions

-20C

Each BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.

To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
The recommended range of input material is in the range of 5’000-50’000 cells.
The only difference between BRB-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the BRB-seq barcode sequence.

For a thorough description of BRB-seq data processing, please refer to the BRB-seq kit user guide. In order to facilate as much as possible BRB-seq data analysis, we have also created a dedicate pipeline which can be found in our “SOFTWARE” page.