MERCURIUS™

DRUG-seq kits

Massively multiplexed and extraction-free library preparation kits

Compatible with Illumina® and AVITI™ sequencers.


Catalog number	#10841	#11041	#10851	#11051
Total preps How many library preps can be prepared in total with one kit	96	384	384	1,536
Sample multiplexing and plate format How many samples can be multiplexed in one single tube	96	96	384	384
Barcoded oligo-dT plates included	1	4	1	4
UDI pairs included How many individual libraries can be sequenced together	4	4	4	4
Documentation	User guide Barcode files

Cat ##10841
Total reactions How many library preps can be prepared in total with one kit	96
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Cat ##11041
Total reactions How many library preps can be prepared in total with one kit	384
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Cat ##10851
Total reactions How many library preps can be prepared in total with one kit	384
RNA multiplexing format How many library preps can be prepared in total with one kit	384
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Cat ##11051
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit	384
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Accessories

Cell lysis module

Optimized lysis buffer for complete lysis and efficient reverse transcription.
Compatible with DRUG-seq library prep kits.

Find out more

UDI expansion module

Compatible with the MERCURIUS™ product line.
12 UDI pairs.
Flexible dual indexing solutions.

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Benefits

The MERCURIUS™ Extraction-free DRUG-seq kits contain all the oligos and enzymes needed to go from 2D cell cultures to sequencing-ready libraries.

Ideal for screening projects: More samples, more replicates. Robust results, significant discoveries.

No need for prior RNA extraction: An optimized lysis buffer for complete lysis and efficient reverse transcription.

Improved protocol: Without pre-amplification, leading to higher mapping and gene detection rates.

One-day lab workflow: Convenient and short protocol from samples to sequencing-ready libraries in one day.

Experimental workflow at a glance
Performance
Product specifications
Validated cell lines

DRUG-seq-2-1

Large-scale analysis of synergistic/antagonistic compound combinations effects

1. Gene detection overview

Gene detection overview of a case study on 12,000 samples and 3,840 compound combinations sequenced at 1M reads per sample.

1. MERCURIUS™ DRUG-seq enables accurate clustering and co-clustering analysis based on unbiased gene expression measurement

upscalemedia-transformed-1

2. Detect potential synergistic/antagonistic effects of drug combinations

For (application)	3' mRNA sequencing
For use with (equipment)	Illumina and AVITI NGS instruments
Species compatibility	All eukaryotic species
Available formats	96, 384 preps 1'536 preps
Shipping conditions	Dry ice
Storage conditions	-20C

Cell line/Tissue	Organism
HT1080 SMARCA4 KO	Human
hTEC	Human
iPSC microglia	Human
MCF7	Human
HepG2_IGF1RKO	Human
Beas-2B	Human
dTHP-1	Human
iPSC derived cardiomyocytes	Human
Endothelial	Human
Hepatocyte	Human
HEPG2	Human
Hepatocyte	Human
SF9	Spodoptera
A549	Human
Breast Cancer (MCF7)	Human
B lymphoblast MM.1s.	Human
Hek293	Human
HeLa Cells	Human
U2-OS	Human
Hek293	Human
dTHP-1	Human
AsPC-1	Human
PBMC (TCD4)	Human
Skeletal muscle (LHCN-M2)	Human
HepaRG	Human
Macrophage (MV-4-11)	Human
PBMCs (Blood)	Human
Microglia	Human
Fibroblast Like Synoviocyte donor 1502	Human
Fibroblast	Human
iPSC-derived neuron	Human
Human lung carcinoma epithelial cell line	Human
Human breast epithelial adenocarcinoma cell line	Human
Human liver hepatocellular carcinoma cell line	Human
Human cardiomyocytes derived from induced pluripotent stem cells (iPSCs)	Human

Large-scale analysis of synergistic/antagonistic compound combinations effects

1. Gene detection overview

Gene detection overview of a case study on 12,000 samples and 3,840 compound combinations sequenced at 1M reads per sample.

1. MERCURIUS™ DRUG-seq enables accurate clustering and co-clustering analysis based on unbiased gene expression measurement

2. Detect potential synergistic/antagonistic effects of drug combinations

Product specifications

Validated cell lines

Sample datasets

HepG2

Number of samples:
360

Reads per sample in demo dataset:
10'000 reads

To have access to the deep-sequenced dataset (31 GB reads per sample) contact us.

Demo dataset file size:
217 MB

Download

Hap1

Number of samples:
24

Reads per sample in demo dataset:
10'000 reads

To have access to the deep-sequenced dataset (7.3 M reads per sample) contact us.

Demo dataset file size:
13.3 MB

Download

HeLa

Number of samples:
24

Reads per sample in demo dataset:
10'000 reads

To have access to the deep-sequenced dataset (7.1 M reads per sample) contact us.

Demo dataset file size:
13.6 MB

Download

FAQs

Each DRUG-seq or BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.

To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
The barcode set for your kit is conveniently located on the kit label. Please refer to the label for accurate identification.

For optimal compatibility, ensure that you use the appropriate plate format (e.g., for kits designed for 96 reactions, the 96 well-plate format should be used). This ensures accurate and efficient processing of your samples. If you have any further questions or concerns, please contact our support team for assistance by email or using our live chat tool.

Relevant publications

Explore the latest, relevant publications in the industry to learn more about our technologies.

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Not ready to use our kits?

Let us do it for you. Our team delivers raw sequencing data (fastq files), gene count matrices and analysis report files. A cost-efficient option suitable for projects of all sizes.

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DRUG-seq kits

Accessories

Cell lysis module

UDI expansion module

Benefits

Sample datasets

HepG2

Hap1

HeLa

FAQs

Relevant publications

Not ready to use our kits?

Speak with one of our RNA experts

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