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Total reactions
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96 | 384 | 384 | 1'536 | 6'144 | 24'576 |
RNA and plate multiplexing format
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96 | 96 | 384 | 384 | 384 | 384 |
Barcoded oligo-dT plates included | 1 | 4 | 1 | 4 | 16 | 64 |
UDI pairs included
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4 | 4 | 4 | 4 | 16 | 16 |
Documentation |
Cat #10841
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---|---|---|---|---|
Total reactions
|
96 | |||
RNA multiplexing format
|
96 | |||
UDI pairs included
|
4 | |||
Cat #11041
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---|---|---|---|---|
Total reactions
|
384 | |||
RNA multiplexing format
|
96 | |||
UDI pairs included
|
4 | |||
Cat #10851
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||||
---|---|---|---|---|
Total reactions
|
384 | |||
RNA multiplexing format
|
384 | |||
UDI pairs included
|
4 | |||
Cat #11051
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||||
---|---|---|---|---|
Total reactions
|
||||
RNA multiplexing format
|
384 | |||
UDI pairs included
|
4 | |||
4x Cat #11051
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||||
---|---|---|---|---|
Total reactions
|
||||
RNA multiplexing format
|
384 | |||
UDI pairs included
|
16 | |||
16x Cat #11051
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---|---|---|---|---|
Total reactions
|
||||
RNA multiplexing format
|
384 | |||
UDI pairs included
|
16 | |||
Benefits
The MERCURIUS™ Extraction-free DRUG-seq kits contain all the oligos and enzymes needed to go from 2D cell cultures to sequencing-ready DNA libraries.
Ideal for screening projects
More samples, more replicates. Robust results, significant discoveries.
No need for prior RNA extraction
An optimized lysis buffer for complete lysis and efficient reverse transcription.
Improved DRUG-seq protocol
Without pre-amplification, leading to higher mapping and gene detection rates.
One-day lab workflow
Convenient and short protocol from samples to sequencing-ready libraries in one day.
Experimental workflow at a glance

Performance
Detection of 12'000+ genes at 1.5 million reads per sample across 384 samples

Distribution of the number of detected genes across...
Detection of 12'000+ genes at 1.5 million reads per sample across 384 samples

Distribution of the number of detected genes across 384 samples prepared with the MERCURIUS™ DRUG-seq library preparation kit. The library was sequenced at an average of 1.5 million reads per sample on an Illumina NovaSeq 6000.
Product Specifications
Sample datasets
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Hap1
Number of samples:24Reads per sample in demo dataset:10'000 readsTo have access to the deep-sequenced dataset (7.3 M reads per sample) contact us.Demo dataset file size:13.3 MBHepG2
Number of samples:65Reads per sample in demo dataset:10'000 readsTo have access to the deep-sequenced dataset (3.9 M reads per sample) contact us.Demo dataset file size:4.76 MBHeLa
Number of samples:24Reads per sample in demo dataset:10'000 readsTo have access to the deep-sequenced dataset (7.1 M reads per sample) contact us.Demo dataset file size:13.6 MB
FAQs
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Each BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.
To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
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The recommended range of input material is in the range of 5’000-50’000 cells.
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The only difference between DRUG-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the DRUG-seq barcode sequence.
For a thorough description of DRUG-seq data processing, please refer to the DRUG-seq kit user guide.
Speak with our RNA sequencing experts
Book a one-on-one call with one of our RNA experts to discover how we can assist your next project.