Spheroid DRUG-seq kits

Massively multiplexed and extraction-free library preparation kits directly from spheroids

Compatible with for Illumina®, AVITI™ and MGI*

* For native kits' compatibility with MGI sequencers, contact us.


Catalog number	#10870	#11670	#10875	#11675
Total preps How many library preps can be prepared in total with one kit	96	384	384	1,536
Sample multiplexing and plate format How many samples can be multiplexed in one single tube	96	96	384	384
Barcoded oligo-dT plates included	1	4	1	4
UDI pairs included How many individual libraries can be sequenced together	4	4	4	4
Documentation	User guide Barcode files

Cat ##10870
Total reactions How many library preps can be prepared in total with one kit	96
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Cat ##11670
Total reactions How many library preps can be prepared in total with one kit	384
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Cat ##10875
Total reactions How many library preps can be prepared in total with one kit	384
RNA multiplexing format How many library preps can be prepared in total with one kit	384
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Cat ##11675
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit	384
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Accessories

Spheroid lysis module

Optimized lysis buffer for complete lysis and efficient reverse transcription.
Compatible with DRUG-seq library prep kits.

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UDI expansion module

Compatible with the MERCURIUS™ product line.
12 UDI pairs.
Flexible dual indexing solutions.

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Benefits

The MERCURIUS™ Extraction-free Spheroid DRUG-seq kits contain all the oligos and enzymes needed to go from 3D cell cultures to sequencing-ready libraries.

Ideal for screening projects: More samples, more replicates. Robust results, significant discoveries.

No need for prior RNA extraction: An optimized lysis buffer for complete lysis and efficient reverse transcription.

Improved protocol: Without pre-amplification, leading to higher mapping and gene detection rates.

One-day lab workflow: Convenient and short protocol from samples to sequencing-ready libraries in one day.

Experimental workflow at a glance
Performance
Product specifications
Validated cell lines

Spheriod DRUG-seq-1

1. Detection of 14'000+ genes at 1.7 million reads per sample across 80 samples

Distribution of the number of detected genes across 80 samples per library, prepared with the MERCURIUS™ Spheroid DRUG-seq library preparation kit from frozen spheroids. The two different libraries were sequenced at an average of 1.7 million reads per sample.

2. Spheroid DRUG-seq shows high demultiplexing rate and high mapping rate to exons

Spheroid DRUG-seq performance for the two libraries shows 95% demultiplexing rate from raw data and 82% mapping rate of the 80 pooled samples, sequenced at 1.7 million reads per sample.

For (application)	3' mRNA sequencing
For use with (equipment)	Illumina and AVITI NGS instruments
Species compatibility	All eukaryotic species
Available formats	96, 384 preps 1'536 preps
Shipping conditions	Dry ice
Storage conditions	-20C

Name	Origin	Species
U2OS	Osteosarcoma	Human
SWR837	Adenocarcinoma	Human
DLD-1	Adenocarcinoma	Human
3D InSight™ Human Liver TOX Model	Liver	Human
3D InSight™ Human Tumor Model	Liver	Human

1. Detection of 14'000+ genes at 1.7 million reads per sample across 80 samples

Distribution of the number of detected genes across 80 samples per library, prepared with the MERCURIUS™ Spheroid DRUG-seq library preparation kit from frozen spheroids. The two different libraries were sequenced at an average of 1.7 million reads per sample.

2. Spheroid DRUG-seq shows high demultiplexing rate and high mapping rate to exons

Spheroid DRUG-seq performance for the two libraries shows 95% demultiplexing rate from raw data and 82% mapping rate of the 80 pooled samples, sequenced at 1.7 million reads per sample.

Product specifications

Validated cell lines

FAQs

Each DRUG-seq or BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.

To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
The barcode set for your kit is conveniently located on the kit label. Please refer to the label for accurate identification.

For optimal compatibility, ensure that you use the appropriate plate format (e.g., for kits designed for 96 reactions, the 96 well-plate format should be used). This ensures accurate and efficient processing of your samples. If you have any further questions or concerns, please contact our support team for assistance by email or using our live chat tool.