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MERCURIUS™

Organoid  DRUG-seq

Extraction-free library preparation kits for Illumina®

RNA-seq library preps directly from organoid lysates without prior RNA isolation.

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IMG_AG_Organoid_DRUG-seq_Kit
Cat #10861 CP_AG_FrontTall_Comp_Transparent_Small
Total reactions How many library preps can be prepared in total with one kit 96
RNA multiplexing format How many library preps can be prepared in total with one kit 96
UDI pairs included How many library preps can be prepared in total with one kit 4
icn_blood-brb-seq
Cat #11061 CP_AG_FrontTall_Comp__Small
Total reactions How many library preps can be prepared in total with one kit 384
RNA multiplexing format How many library preps can be prepared in total with one kit 96
UDI pairs included How many library preps can be prepared in total with one kit 4
icn_blood-brb-seq
Cat #10871 img_kit
Total reactions How many library preps can be prepared in total with one kit 384
RNA multiplexing format How many library preps can be prepared in total with one kit 384
UDI pairs included How many library preps can be prepared in total with one kit 4
icn_blood-brb-seq
Cat #11071 CP_AG_FrontTall_Comp__Small
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit 384
UDI pairs included How many library preps can be prepared in total with one kit 4
icn_blood-brb-seq
4x Cat #11071 CP_AG_FrontTall_Comp__Small
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit 384
UDI pairs included How many library preps can be prepared in total with one kit 4
icn_blood-brb-seq
16x Cat #11071 CP_AG_FrontTall_Comp__Small
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit 384
UDI pairs included How many library preps can be prepared in total with one kit 4
icn_blood-brb-seq

Kit Pricing

Discover the unique scalability offered by Organoid DRUG-seq

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Custom Format

For more reactions, higher multiplexing and more UDIs

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Benefits

The MERCURIUS™ Extraction-free Organoid DRUG-seq kits contain all the oligos and enzymes needed to go from 3D organoids and spheroids to sequencing-ready DNA libraries

ideal for screening projects

Drug-seq ideal for screening projects

More samples, more replicates. Robust results, significant discoveries.

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Streamlined data pre-processing

Demultiplex and align your DRUG-seq data with our easy-to-use cloud-based platform.

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No need for prior RNA extraction

An optimized lysis buffer for complete lysis and efficient reverse transcription.

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Improved DRUG-seq protocol

Without pre-amplification, leading to higher mapping and gene detection rates.

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One-day lab workflow

Convenient and short protocol from samples to sequencing-ready libraries in one day.

Experimental workflow at a glance

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Product Specifications

For use with (application)
3' mRNA sequencing
For use with (equipment)
Illumina NGS instruments
Species compatibility
All eukaryotic species
Available formats
96, 384, 1'536, 6'144, 36'864 reactions
Shipping conditions
Dry ice
Storage conditions
-20C

FAQs

  • Each BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.

    To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.

  • The recommended range of input material is in the range of 5’000-50’000 cells.
  • The only difference between BRB-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the BRB-seq barcode sequence.

    For a thorough description of BRB-seq data processing, please refer to the BRB-seq kit user guide. In order to facilate as much as possible BRB-seq data analysis, we have also created a dedicate pipeline which can be found in our “SOFTWARE” page.

Try our services

Our BRB-seq and DRUG-seq service deliver raw sequencing data (fastq files), gene count matrices and analysis report files. A cost-efficient option suitable for projects of all sizes.

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Book a one-on-one call with one of our RNA experts to discover how we can assist your next project.