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MERCURIUS™

Organoid DRUG-seq 

Multiplexed extraction-free library preparation kits for Illumina®

RNA-seq library preps directly from organoid lysis without prior RNA isolation.

Cat #10861
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit 96
UDI pairs included How many library preps can be prepared in total with one kit 4
Cat #11061
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit 96
UDI pairs included How many library preps can be prepared in total with one kit 4
Cat #10871
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit 384
UDI pairs included How many library preps can be prepared in total with one kit 4
Cat #11071
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit 384
UDI pairs included How many library preps can be prepared in total with one kit 4
Cat #12071
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit 384
UDI pairs included How many library preps can be prepared in total with one kit 16
Cat #13071
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit 384
UDI pairs included How many library preps can be prepared in total with one kit 16

Add-ons

Accessories

Organoid lysis
module

 

 

organoid

 


  • Optimized lysis buffer for complete lysis and efficient reverse transcription.
  • Compatible with DRUG-seq library prep kits. 

Accessories

UDI expansion
module

 

 

Asset 1-Jun-21-2023-09-58-09-8501-AM

 

 

  • Compatible with the MERCURIUS™ product line.
  • 12 UDI pairs.   
  • Flexible dual indexing solutions. 

Package

Libraries
sequencing

$3

per million reads

 


  • Take advantage of our low sequencing price, which is enabled by the recurrent sequencing runs. 
  • Your libraries will be sequenced in large sequencing runs with other compatible BRB-seq and DRUG-seq libraries.

 

Package

Data
pre-processing

 $600 NOW $150

per 96 samples

 

  • We provide you with everything from raw data to gene count matrices and sequencing report.
  • Guaranteed turn-on time: 2 days. 

  • Benefits

    The MERCURIUS™ Extraction-free Organoid DRUG-seq kits contain all the oligos and enzymes needed to go from 3D organoids and spheroids to sequencing-ready DNA libraries

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    benefits_NoRNAExt_gradient

     

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    benefits_workflow_gradient

     

    Ideal for screening projects No need for prior RNA extraction Improved DRUG-seq protocol

    One-day lab workflow

    More samples, more replicates. Robust results, significant discoveries.

    An optimized lysis buffer for complete lysis and efficient reverse transcription.

    Without pre-amplification, leading to higher mapping and gene detection rates.

    Convenient and short protocol. From samples to sequencing-ready libraries in one day.

     

  • Experimental workflow at a glance

    Organoid-1

     

  • Product specifications

     

    For (application)

    3' mRNA sequencing

    For use with (equipment)

    Illumina NGS instruments

    Species compatibility

    All eukaryotic species

    Available formats

    96, 384, 1'536, 6'144 and 24'576 reactions

    Shipping conditions

    Dry ice

    Storage conditions

    -20C

     

Sample dataset

  • Organoids

    Prostate cancer

    Number of samples:
    12
     
    Reads per sample in demo dataset:
    10'000 reads
     
    To have access to the deep-sequenced dataset (24.5M reads per sample) contact us.
     
    Demo dataset file size:
    7.16 MB
    Download

FAQs

  • Each BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.

    To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.

  • The recommended range of input material is in the range of 5’000-50’000 cells.
  • The only difference between DRUG-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the DRUG-seq barcode sequence.

    For a thorough description of DRUG-seq data processing, please refer to the DRUG-seq kit user guide. 

  • The barcode set for your kit is conveniently located on the kit label. Please refer to the label for accurate identification.

    For optimal compatibility, ensure that you use the appropriate plate format (e.g., for kits designed for 96 reactions, the 96 well-plate format should be used). This ensures accurate and efficient processing of your samples. If you have any further questions or concerns, please contact our support team for assistance by email or using our live chat tool.

Try our services

Our BRB-seq and DRUG-seq service deliver raw sequencing data (fastq files), gene count matrices and analysis report files. A cost-efficient option suitable for projects of all sizes.

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