MERCURIUS™

Organoid DRUG-seq kits

Multiplexed extraction-free library preparation kits for Illumina® and AVITI™

RNA-seq library preps directly from organoids without prior RNA isolation.


Catalog number	#10861	#11061	#10871	#11071
Total preps How many library preps can be prepared in total with one kit	96	384	384	1,536
Sample multiplexing and plate format How many samples can be multiplexed in one single tube	96	96	384	384
Barcoded oligo-dT plates included	1	4	1	4
UDI pairs included How many individual libraries can be sequenced together	4	4	4	4
Documentation	User guide Barcode files

Cat ##10861
Total reactions How many library preps can be prepared in total with one kit	96
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Cat ##11061
Total reactions How many library preps can be prepared in total with one kit	384
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Cat ##10871
Total reactions How many library preps can be prepared in total with one kit	384
RNA multiplexing format How many library preps can be prepared in total with one kit	384
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Cat ##11071
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit	384
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Accessories

Organoid lysis module

Optimized lysis buffer for complete lysis and efficient reverse transcription.
Compatible with DRUG-seq library prep kits.

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UDI expansion module

Compatible with the MERCURIUS™ product line.
12 UDI pairs.
Flexible dual indexing solutions.

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Benefits

The MERCURIUS™ Extraction-free Organoid DRUG-seq kits contain all the oligos and enzymes needed to go from 3D organoids and spheroids to sequencing-ready libraries

Ideal for screening projects: More samples, more replicates. Robust results, significant discoveries.

No need for prior RNA extraction: An optimized lysis buffer for complete lysis and efficient reverse transcription.

Improved DRUG-seq protocol: Without pre-amplification, leading to higher mapping and gene detection rates.

One-day lab workflow: Convenient and short protocol from samples to sequencing-ready libraries in one day.

Experimental workflow at a glance
Product specifications

For (application)	3' mRNA sequencing
For use with (equipment)	Illumina and AVITI NGS instruments
Species compatibility	All eukaryotic species
Available formats	96, 384 and 1'536 preps
Shipping conditions	Dry ice
Storage conditions	-20C

Product specifications

Sample dataset

Prostate cancer

Number of samples:
12

Reads per sample in demo dataset:
10'000 reads

To have access to the deep-sequenced dataset (24.5M reads per sample) contact us.

Demo dataset file size:
7.16 MB

Download

FAQs

Each DRUG-seq or BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.

To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
The barcode set for your kit is conveniently located on the kit label. Please refer to the label for accurate identification.

For optimal compatibility, ensure that you use the appropriate plate format (e.g., for kits designed for 96 reactions, the 96 well-plate format should be used). This ensures accurate and efficient processing of your samples. If you have any further questions or concerns, please contact our support team for assistance by email or using our live chat tool.

Relevant publications

Explore the latest, relevant publications in the industry to learn more about our technologies.

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Try our service

Our Organoid DRUG-seq service delivers raw sequencing data (fastq files), gene count matrices and analysis report files. A cost-efficient option suitable for projects of all sizes.

COMPLEMENTARY SERVICE

Cells and organoids (Extraction-free)

Purified RNA

Accessories

Cells and Organoids (EXTRACTION-FREE)

PURIFIED RNA

Whole Blood RNA

Cells and organoids (Extraction-free)

Purified RNA

Accessories

Cells and Organoids (EXTRACTION-FREE)

PURIFIED RNA

Whole Blood RNA

Organoid DRUG-seq kits

Accessories

Organoid lysis module

UDI expansion module

Benefits

Sample dataset

Prostate cancer

FAQs

Relevant publications

Try our service

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