MERCURIUS™ Full-length BRB-seq kit for Illumina® and AVITI™


Catalog number	#10801	#11601
Total preps How many library preps can be prepared in total with one kit	96	384
RNA and plate multiplexing format How many samples can be multiplexed in one single tube	96	96
Barcoded oligo-dT plates included	1	4
UDI pairs included How many individual libraries can be sequenced together	4	4
Documentation	User guide	Barcode files

Cat ##10801
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Cat ##11601
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Accessories

UDI expansion

module

Compatible with the MERCURIUS™ product line.
12 UDI pairs.
Flexible dual indexing solutions.

Find out more

Benefits

The MERCURIUS™ Full-Length BRB-seq library preparation kits for Illumina® and AVITI contain all the oligos and enzymes needed to go from purified RNA to sequencing-ready libraries.


Multiplexed full-length mRNA-seq	Full-length transcript coverage	One-day lab workflow
Full-length mRNA-seq library prep, 96 samples in a single tube.	From differential gene expression to splicing variants and isoform detection.	Convenient and short protocol from samples to sequencing-ready libraries in one day.

Experimental workflow at a glance
Performance

1. Uniform detection of 22'000+ genes at 12 million reads per sample across 48 samples

Distribution of the number of detected genes across 48 samples prepared with the MERCURIUS™ Full-Length BRB-seq service. The library was sequenced at an average of 12 million reads.

2. Full-Length BRB-seq shows high demultiplexing rate and high mapping rate to exons

Full-Length BRB-seq performance shows 99% demultiplexing rate from raw data, 78% mapping rate, and 18% duplication rate of the 48 pooled samples and sequenced at 12 million reads per sample.

3. Mapping rates as high as 80% for different RNA inputs

Mapping rates are uniform across three RNA inputs tested: 1ug, 10ng, and 100ng. Human Universal Reference RNA was used to prepare mRNA Full-Length BRB libraries.

4. Full-Length BRB-seq exhibits reads distribution across the transcript’s entire length

The gene body coverage shows a consistent and uniform reads distribution across the entire gene body for the Full-Length BRB-seq protocol, comparable to competitor N protocol, while the BRB-seq protocol shows a significant 3' bias due to its poly-A selection methodology.

5. >50'000 isoforms detected at 15M sequencing depth

Benchmarking the Full-Length BRB-seq protocol against competitor N shows similar performance for isoform detection. More than 50,000 isoforms can be detected at a sequencing depth of 12M reads per sample.

Product specifications

For (application)	Full-Length mRNA sequencing
For use with (equipment)	Illumina and AVITI NGS instruments
Species compatibility	All eukaryotic species
Available formats	96 and 384 preps
Shipping conditions	Dry ice
Storage conditions	-20C

Human

Number of samples: 48

Reads per sample in demo dataset:

10'000 reads

To have access to the deep-sequenced dataset contact us.

Demo dataset file size:

9.9 MB

Each Full-Length BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.

For instance, the 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
The recommended range of RNA amount for each sample is of 10-1000 ng, normally the more RNA, the better.

The minimum recommended RIN number is 6 and the A260/230 ratio (Nanodrop) should be in the 1.5-2.2 range.
The only difference between Full-Length BRB-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the BRB-seq barcode sequence.

For a thorough description of Full-Length BRB-seq data processing, please look at the BRB-seq kit user guide.
Full-Length BRB-seq provides comprehensive coverage of the full-length mRNA transcripts.

We, therefore, normally recommend sequencing 12-20 million reads for each sample, which enables the reliable and unbiased detection of over 20,000 genes.
The barcode set for your kit is conveniently located on the kit label. Please refer to the label for accurate identification.

For optimal compatibility, ensure that you use the appropriate plate format (e.g., for kits designed for 96 reactions, the 96 well-plate format should be used). This ensures accurate and efficient processing of your samples. If you have any further questions or concerns, please contact our support team for assistance by email or using our live chat tool.

MERCURIUS™

Full-Length BRB-seq kits (BETA)

Library preparation kits for Illumina^®and AVITI™