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MERCURIUS™

Targeted BRB-seq kits

Massively multiplexed and targeted RNA-seq from purified RNA samples - compatible with all eukaryotic species.

Compatible with Illumina® , AVITI™ and MGI* sequencing systems.

* For native kits' compatibility with MGI sequencers, contact us.

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Benefits

The MERCURIUS™ Targeted BRB-seq kits  offer the most comprehensive, high-throughput, and cost-efficient solution for targeted gene expression analysis projects. The kits contain all the oligos and enzymes needed to go from purified RNA to sequencing-ready libraries.
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Custom-developed targeted panels

Users communicate their targets of interest, and we will develop ad-hoc detection panels.

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Up to 100 targets

Experimentally validated panels for differential expression analysis.

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Bulk RNA sequencing at scale

Perform up-to 384 RNA-seq library preparations in one single tube.

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One-day lab workflow

Convenient and short protocol from samples to sequencing-ready libraries in one day.

What we do

MERCURIUS ™

Targeted BRB-seq kits


The production of our Targeted BRB-seq kits starts with the client providing us with their genes of interest. We then develop a corresponding custom detection panel and validate it directly using their RNA samples.

During the development process, the client provides us with purified RNA samples for gene panel validation.

Once the targeted gene panel validation is complete, we will notify the client and ship a pilot batch of Targeted BRB-seq library prep kits. Upon receiving the client's approval, we will commence production of the custom kit batch.

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How does it work

  • Client communicates the genes of interest panel

    Gene panel submission form for Targeted BRB-seq or Targeted DRUG-seq
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  • Client ships the purified RNA samples to Alithea for panel validation

    Sample submission guidelines for MERCURIUSTM  Targeted BRB-seq kits
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  • 4 to 8 weeks

    Alithea develops and validates the custom detection panel on customer's samples

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  • Client checkpoint - Alithea ships a pilot batch

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  • Upon client's approval, Alithea begins the dedicated batch production of custom kits

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  • Performance

    1. Improvement of target gene detection with Targeted BRB-seq

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    Gene ranked by detection abundance with MERCURIUS™ Targeted BRB-seq on a panel of 24 target genes compared to MERCURIUS™ BRB-seq.
     
     

  • Product specifications

     

    For (application)

    Targeted RNA sequencing

    For use with (equipment)

    Illumina® , AVITI™ and MGI instruments

    Species compatibility

    All eukaryotic species

    Shipping conditions

    Dry ice

    Storage conditions

    -20C

     

FAQs

  • Each Targeted BRB-seq contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient to complete the library preparation process for four different BRB-seq pools.

    To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-sample kit can be used to prepare up to 96 samples distributed across up to four different libraries.

  • To guarantee high-quality data, we normally request that each sample contains at least 200ng of total RNA in at least 10μl.

  • Targeted DRUG-seq is a 3’-end RNA sequencing method that requires significantly less sequencing than standard BRB-seq to achieve accurate gene quantification. Therefore, we recommend sequencing 100 reads per target for each sample, which enables reliable detection of the targeted genes.
  • The only difference between BRB-seq and standard RNA-seq data analysis is the demultiplexing step, which assigns sequencing reads to their sample of origin based on the BRB-seq barcode sequence.

Try our service

The MERCURIUS™ Targeted BRB-seq service offers the most comprehensive, high-throughput and cost-efficient solution for targeted gene expression analysis projects starting from purified RNA.

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Speak with our RNA sequencing experts

Book a one-on-one call with one of our RNA experts to discover how we can assist your next project.