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MERCURIUS™

Full-Length BRB-seq service

Full-length mRNA-seq combined with massive sample multiplexing.

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Benefits

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Multiplexed full-length mRNA-seq

Full-length mRNA-seq library prep, 96 samples in a single tube.

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Full-length transcript coverage

From differential gene expression to splicing variants and isoform detection.

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Fast turnaround times

From samples to data in as little as one month.

What we do

MERCURIUS ™

Full-Length BRB-seq service


The MERCURIUS™ Full-Length BRB-seq service offers a convenient and streamlined solution for full-length mRNA transcriptomics projects of any size.

Clients can send us purified RNA samples, which are always quality-checked before launching our Full-Length BRB-seq pipeline. During the process, we always keep clients informed at defined checkpoints so that we can decide together how to best proceed to the next steps.

Next generation sequencing and data pre-processing (including alignment to the genome of choice) are part of our standard service as well. As a result, we provide our clients raw data, sequencing and alignment reports, and gene count matrices which can be used for downstream gene expression analysis.

Optional downstream differential gene expression analysis are also available upon request.

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How does it work

  • Client prepares the samples

    Sample preparation guidelines for purified RNA (PDF)
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  • Client sends submission form

    Sample submission form for MERCURIUSTM  Full-Length BRB-seq service
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  • Client ships samples to Alithea

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  • 1 week

    Incoming QC - Client Checkpoint

    (Nanodrop, Ribogreen, Tapestation)
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  • 2 days

    MERCURIUS™ Full-Length BRB-seq library preparation

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  • 1 week

    Library QC - Client Checkpoint

    (Qubit, Fragment analyzer, shallow sequencing)
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  • 1 week

    Deep sequencing

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  • 1 week

    Data analysis and reporting - Client Checkpoint

    Example of sample report
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  • Data

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  • Optional: Alithea performs Differential Gene Expression analysis​

Performance

FAQs

  • To generate high-quality sequencing data we recommend starting with 10 - 1000 ng of total purified RNA per sample. 

    The total RNA amount per pool should be at least 1000 ng.

  • In addition to total RNA amount, it is important that the samples contain RNA of high integrity (RIN > 6) and are devoid of contaminants (Nanodrop A260/A230 between 1.8 and 2.2).
  • Full-Length BRB-seq provides comprehensive coverage of the full-length mRNA transcripts.

    We, therefore, normally recommend sequencing 12-20 million reads for each sample, which enables the reliable and unbiased detection of over 20,000 genes.

  • As part of our standard service pipeline, we align the generated data to the genome of choice, provide a detailed report on the alignment and gene counting statistics and, finally, provide ready-to-use gene count matrices for downstream analysis.

    Optionally, we can include differential gene expression analysis.

See our products

Our Full-Length BRB-seq kits combine the full-length mRNA-seq with massive sample multiplexing while providing the most cost-effective solution.

Experience sample to sequencing-ready library in one day.

Discover our kits
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Speak with our RNA sequencing experts

Need guidance or have questions about BRB-seq and our service?

Easily book a call with our BRB-seq experts.