Full-length BRB-seq Services | RNA-seq Service

Benefits

Multiplexed full-length mRNA-seq: Full-length mRNA-seq library prep, 96 samples in a single tube.

Full-length transcript coverage: From differential gene expression to splicing variants and isoform detection.

Fast turnaround times: From samples to data in as little as six weeks.

MERCURIUS^TM

Full-Length BRB-seq service

The MERCURIUS™ Full-Length BRB-seq service offers a convenient and streamlined solution for full-length mRNA transcriptomics projects of any size.

Clients can send us purified RNA samples, which are always quality-checked before launching our Full-Length BRB-seq pipeline. During the process, we always keep clients informed at defined checkpoints so that we can decide together how to best proceed to the next steps.

Next generation sequencing and data pre-processing (including alignment to the genome of choice) are part of our standard service as well. As a result, we provide our clients raw data, sequencing and alignment reports, and gene count matrices which can be used for downstream gene expression analysis.

Optional downstream differential gene expression analysis are also available upon request.

How it works

Step 1

Client prepares the samples

Sample preparation guidelines for purified RNA (PDF)

Step 2

Client sends submission form

Sample submission form for MERCURIUS^TM Full-Length BRB-seq service

Step 3

Client ships samples to Alithea

Step 4

Incoming QC - Client Checkpoint

(Nanodrop and Fragment Analyzer)

1 week

Step 5

MERCURIUS™ Full-Length BRB-seq library preparation

2 days

Step 6

Library QC - Client Checkpoint

(Qubit, Fragment analyzer, shallow sequencing)

1 week

Step 7

Deep sequencing

1 week

Step 8

Data analysis and reporting - Client Checkpoint

Example of sample report

1 week

Step 9

Data

Optional: Alithea performs Differential Gene Expression analysis

Performance

Uniform detection of 22'000+ genes at 12 million reads per sample across 48 samples

Distribution of the number of detected genes across 48 samples prepared with the MERCURIUS™ Full-Length BRB-seq service. The library was sequenced at an average of 12 million reads.

Full-Length BRB-seq shows high demultiplexing rate and high mapping rate to exons

Full-Length BRB-seq performance shows 99% demultiplexing rate from raw data, 78% mapping rate, and 18% duplication rate of the 48 pooled samples and sequenced at 12 million reads per sample.

Mapping rates as high as 80% for different RNA inputs

Mapping rates are uniform across three RNA inputs tested: 1ug, 10ng, and 100ng. Human Universal Reference RNA was used to prepare mRNA Full-Length BRB libraries.

Full-Length BRB-seq exhibits reads distribution across the transcript’s entire length

The gene body coverage shows a consistent and uniform reads distribution across the entire gene body for the Full-Length BRB-seq protocol, comparable to the NEB protocol, while the BRB-seq protocol shows a significant 3' bias due to its poly-A selection methodology.

>50'000 isoforms detected at 15M sequencing depth

Benchmarking the Full-Length BRB-seq protocol against the competitor NEB shows similar performance for isoform detection. More than 50,000 isoforms can be detected at a sequencing depth of 12M reads per sample.

FAQs

Relevant publications

Explore the latest, relevant publications in the industry to learn more about BRB-seq.

Explore

Want to do Full-Length BRB-seq in-house?

Our Full-Length BRB-seq kits combine the full-length mRNA-seq with massive sample multiplexing while providing the most cost-effective solution.

Experience sample to sequencing-ready library in one day.

complementary kit

Cells and organoids (Extraction-free)

Purified RNA

Accessories

Cells and Organoids (EXTRACTION-FREE)

PURIFIED RNA

Whole Blood RNA

Cells and organoids (Extraction-free)

Purified RNA

Accessories

Cells and Organoids (EXTRACTION-FREE)

PURIFIED RNA

Whole Blood RNA

Full-Length BRB-seq service

Benefits

Full-Length BRB-seq service

How it works

Client prepares the samples

Client sends submission form

Client ships samples to Alithea

Incoming QC - Client Checkpoint

MERCURIUS™ Full-Length BRB-seq library preparation

Library QC - Client Checkpoint

Deep sequencing

Data analysis and reporting - Client Checkpoint

Data

Optional: Alithea performs Differential Gene Expression analysis

Performance

Uniform detection of 22'000+ genes at 12 million reads per sample across 48 samples

Full-Length BRB-seq shows high demultiplexing rate and high mapping rate to exons

Mapping rates as high as 80% for different RNA inputs

Full-Length BRB-seq exhibits reads distribution across the transcript’s entire length

>50'000 isoforms detected at 15M sequencing depth

FAQs

Relevant publications

Want to do Full-Length BRB-seq in-house?

Speak with one of our RNA experts

Subscribe to our newsletter

Get in touch

Full-Length BRB-seq service

Benefits

Full-Length BRB-seq service

How it works

Client prepares the samples

Client sends submission form

Client ships samples to Alithea

Incoming QC - Client Checkpoint

MERCURIUS™ Full-Length BRB-seq library preparation

Library QC - Client Checkpoint

Deep sequencing

Data analysis and reporting - Client Checkpoint

Data

Optional: Alithea performs Differential Gene Expression analysis​

Performance

Uniform detection of 22'000+ genes at 12 million reads per sample across 48 samples

Full-Length BRB-seq shows high demultiplexing rate and high mapping rate to exons

Mapping rates as high as 80% for different RNA inputs

Full-Length BRB-seq exhibits reads distribution across the transcript’s entire length

>50'000 isoforms detected at 15M sequencing depth

FAQs

Relevant publications

Want to do Full-Length BRB-seq in-house?

Speak with one of our RNA experts

Subscribe to our newsletter

Optional: Alithea performs Differential Gene Expression analysis