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MERCURIUS™

High sensitivity BRB-seq
Library preparation kits
for Illumina®

The power of massively multiplexed RNA-seq, now compatible with low-input RNA samples, as low as 1ng per well.

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Asset 1-Jul-06-2023-09-31-57-4180-AM-2
Cat #10881
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit 96
UDI pairs included How many library preps can be prepared in total with one kit 4
Cat #10891 img_kit
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit 384
UDI pairs included How many library preps can be prepared in total with one kit 4

Benefits

The generated 3' mRNA-seq data is ideally suited for differential gene expression analysis.

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High sensitivity for low-input RNA

From as low as 1ng/well.

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Bulk RNA sequencing at scale

Perform up-to 384 RNA-seq library preparations in one single tube.

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One-day
lab
workflow

Convenient and short protocol from samples to sequencing-ready libraries in one day.

Experimental workflow at a glance

HS BRB-seq workflow-3

High Sensitivity BRB-seq or standard BRB-seq?

  • Selection guide

    How to identify the best-performing BRB-seq method for your project.
     
    HS BRB-seq kit selection guide-2-1 

    Table 1. Recommended BRB-seq method depending on the RNA input per well

    and the number of samples to be pooled. 

  • Performance

     Comparison of library complexity between BRB-seq and HS BRB-seq at the same sequencing depth.
     

     

    Figure 1. Number of detected genes between standard BRB-seq and

    High Sensitivity BRB-seq at different RNA inputs per sample.

    96 samples were pooled together and sequenced at 2M reads/sample.

    To note: all replicates were prepared from the same RNA sample.

Product Specifications

For (application)
3' mRNA sequencing
For use with (equipment)
Illumina NGS instruments
Species compatibility
All eukaryotic species
Available formats
96, 384 reactions
Shipping conditions
Dry ice
Storage conditions
-20C

Not ready to use our kits?

No problem, send us your samples and we will generate the data for you! Thanks to BRB-seq and DRUG-seq our service is the most cost-efficient and rapid solution on the market.

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