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High sensitivity BRB-seq
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MERCURIUS™

High sensitivity BRB-seq

Library preparation kits for Illumina®

The power of massively multiplexed RNA-seq, now compatible with low-input RNA samples, as low as       1 ng per well.

Cat ##10881
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit 96
UDI pairs included How many library preps can be prepared in total with one kit 4
Cat ##10891
Total reactions How many library preps can be prepared in total with one kit 384
RNA multiplexing format How many library preps can be prepared in total with one kit 384
UDI pairs included How many library preps can be prepared in total with one kit 4

Add-ons

Accessories

UDI expansion
module

 

 

Asset 1-Jun-21-2023-09-58-09-8501-AM

 

 

  • Compatible with the MERCURIUS™ product line.
  • 12 UDI pairs.   
  • Flexible dual indexing solutions. 

Package

Libraries
sequencing

$3

per million reads

 


  • Take advantage of our low sequencing price, which is enabled by the recurrent sequencing runs. 
  • Your libraries will be sequenced in large sequencing runs with other compatible BRB-seq and DRUG-seq libraries.

 

Package

Data
pre-processing

 $600 NOW $150

per 96 samples

 

  • We provide you with everything from raw data to gene count matrices and sequencing report.
  • Guaranteed turn-on time: 2 days. 

  • Benefits

    The generated 3' mRNA-seq data is ideally suited for differential gene expression analysis.

    product_brbseq_gradient

     

    benefits_bulk_gradient_64x64

     

    benefits_workflow_gradient

     

    High sensitivity for
    low-input RNA
    Bulk RNA sequencing 
    at scale
    One-day lab
    workflow 

    From as low as 1 ng/well.

     

    Perform up-to 384 RNA-seq library preparations in one single tube.

    Convenient and short protocol from samples to sequencing-ready libraries in one day.

     

  • Experimental workflow at a glance

    HS BRB-seq-1-1

     

  • Selection guide

    How to identify the best-performing BRB-seq method for your project.

     
    HS BRB-seq kit selection guide-2-1 
    The recommended BRB-seq method depends on the RNA input per well and the number of samples to be pooled. 

     

  • Performance

    Comparison of library complexity between BRB-seq and HS BRB-seq at the same sequencing depth

     

    Number of detected genes between standard BRB-seq and High Sensitivity BRB-seq at different RNA inputs per sample.

    96 samples were pooled together and sequenced at 2M reads/sample.

    To note: all replicates were prepared from the same RNA sample.

  • Product specifications

    For (application)

    3' mRNA sequencing

    For use with (equipment)

    Illumina NGS instruments

    Species compatibility

    All eukaryotic species

    Available formats

    96, 384 reactions

    Shipping conditions

    Dry ice

    Storage conditions

    -20C

FAQs

  • Each BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.

    To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.

  • The recommended range of RNA amount for each sample, using the High Sensitivity BRB-seq library prep kit,  is as low as 1ng per well, normally, the more RNA, the better.

    The minimum recommended RIN number is 6 and the A260/230 ratio (Nanodrop) should be in the 1.5-2.2 range.

  • The only difference between BRB-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the BRB-seq barcode sequence.

    For a thorough description of BRB-seq data processing, please refer to the BRB-seq kit user guide. 

  • One of the key advantages of BRB-seq is that it does not only save reagents and cost in the library preparation stage, but also in the sequencing one.

    As opposed to standard RNA-seq, where 20M-30M reads per sample are required, we normally recommend to sequence BRB-seq libraries at a depth of 4M-5M reads per sample, which is normally enough to detect the vast majority of expressed genes.

  • The barcode set for your kit is conveniently located on the kit label. Please refer to the label for accurate identification.

    For optimal compatibility, ensure that you use the appropriate plate format (e.g., for kits designed for 96 reactions, the 96 well-plate format should be used). This ensures accurate and efficient processing of your samples. If you have any further questions or concerns, please contact our support team for assistance by email or using our live chat tool.

Not ready to use our kits?

No problem, send us your samples and we will generate the data for you! Thanks to BRB-seq and DRUG-seq our service is the most cost-efficient and rapid solution on the market.

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