High sensitivity BRB-seq kit


Catalog number	#10881	#10891
Total preps How many library preps can be prepared in total with one kit	96	384
RNA and plate multiplexing format How many samples can be multiplexed in one single tube	96	384
Barcoded oligo-dT plates included	1	1
UDI pairs included How many individual libraries can be sequenced together	4	4
Documentation	User guide	Barcode files

Cat ##10881
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Cat ##10891
Total reactions How many library preps can be prepared in total with one kit	384
RNA multiplexing format How many library preps can be prepared in total with one kit	384
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Accessories

UDI expansion

module

Compatible with the MERCURIUS™ product line.
12 UDI pairs.
Flexible dual indexing solutions.

Find out more

Benefits

The generated 3' mRNA-seq data is ideally suited for differential gene expression analysis.

High sensitivity for

low-input RNA

Bulk RNA sequencing

at scale

One-day lab

workflow

From as low as 1 ng/well.

Perform up-to 384 RNA-seq library preparations in one single tube.

Convenient and short protocol from samples to sequencing-ready libraries in one day.

Experimental workflow at a glance
Selection guide

How to identify the best-performing BRB-seq method for your project.

The recommended BRB-seq method depends on the RNA input per well and the number of samples to be pooled.
Performance

Comparison of library complexity between BRB-seq and HS BRB-seq at the same sequencing depth

Number of detected genes between standard BRB-seq and High Sensitivity BRB-seq at different RNA inputs per sample.

96 samples were pooled together and sequenced at 2M reads/sample.

To note: all replicates were prepared from the same RNA sample.

Product specifications

For (application)	3' mRNA sequencing
For use with (equipment)	Illumina and AVITI NGS instruments
Species compatibility	All eukaryotic species
Available formats	96, 384 reactions
Shipping conditions	Dry ice
Storage conditions	-20C

Each BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.

To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
The recommended range of RNA amount for each sample, using the High Sensitivity BRB-seq library prep kit, is as low as 1ng per well, normally, the more RNA, the better.

The minimum recommended RIN number is 6 and the A260/230 ratio (Nanodrop) should be in the 1.5-2.2 range.
The only difference between BRB-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the BRB-seq barcode sequence.

For a thorough description of BRB-seq data processing, please refer to the BRB-seq kit user guide.
One of the key advantages of BRB-seq is that it does not only save reagents and cost in the library preparation stage, but also in the sequencing one.

As opposed to standard RNA-seq, where 20M-30M reads per sample are required, we normally recommend to sequence BRB-seq libraries at a depth of 4M-5M reads per sample, which is normally enough to detect the vast majority of expressed genes.
The barcode set for your kit is conveniently located on the kit label. Please refer to the label for accurate identification.

For optimal compatibility, ensure that you use the appropriate plate format (e.g., for kits designed for 96 reactions, the 96 well-plate format should be used). This ensures accurate and efficient processing of your samples. If you have any further questions or concerns, please contact our support team for assistance by email or using our live chat tool.

MERCURIUS™

High Sensitivity BRB-seq kits

Library preparation kits for Illumina^®and AVITI™