BRB-seq
Library preparation kits for Illumina^®

Each BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.

To note, the total number of RNA samples that can be processed with one kit does exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.

The recommended range of RNA amount for each sample is of 50ng-1μg, normally the more RNA, the better.

The minimum recommended RIN number is 6 and the A260/230 ratio (Nanodrop) should be in the 1.5-2.2 range.

The only difference between BRB-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the BRB-seq barcode sequence.

For a thorough description of BRB-seq data processing, please refer to the BRB-seq kit user guide. In order to facilate as much as possible BRB-seq data analysis, we have also created a dedicate pipeline which can be found in our “SOFTWARE” page.

One of the key advantages of BRB-seq is that it does not only save reagents and cost in the library preparation stage, but also in the sequencing one.

As opposed to standard RNA-seq, where 20M-30M reads per sample are required, we normally recommend to sequence BRB-seq libraries at a depth of 4M-5M reads per sample, which is normally enough to detect the vast majority of expressed genes.