MERCURIUS™
Single-Cell FLASH-seq Service
for high-sensitivity single-cell RNA-seq
This service provides full-length mRNA transcript coverage even for low-abundance genes, empowering researchers to explore differential gene expression, detect alternative splicing, and analyze isoform diversity without RNA extraction.
Discover Sample Reports and Datasets
PBMCs, HEK293
Ultra-sensitive
Full-length transcript coverage
For rare cell populations
Ideal for large
RNA-seq projects
About the service
The MERCURIUS™ Single-Cell FLASH-seq service enables researchers to detect full-length mRNA transcripts, alternative splicing events, and isoform diversity even for low-abundance genes and rare cell types crucial to disease biology.
To use the Single-Cell FLASH-seq service, clients must first sort cells and deposit them in the center of each well of our dedicated 96- or 384-well plates. The plates already contain our proprietary buffer for efficient cell lysis and RNA solubilization. These plates must then be frozen and delivered to our service centers located in Switzerland and the United States.
at 0.5M reads/sample
1. Alithea ships the plate(s)
The wells of the plate(s) contain the lysis buffer.
2. Client FACS-sorts the cells and freezes the plate(s)
3. Client ships samples to Alithea
4. MERCURIUS™ FLASH-seq library prep
2 days
5. Library QC - Client Checkpoint
(Fragment analyzer)
1 week
6. Deep Sequencing on AVITI or Illumina, depending on the request by the client
1 week
7. Data analysis and reporting- Client Checkpoint
1 week
8. Data Delivery
Raw FASTQ files, sample report file, QC files, and gene count tables
MERCURIUS™ FLASH-seq Detects More Genes Than Smart-seq3 and Smart-seq2 with Greater Consistency
FLASH-seq detects a median of approximately 11,000 genes per sample in HEK293T cells, outperforming Smart-seq3 and Smart-seq2 after downsampling all to 500k reads/sample.
The Sensitivity Of MERCURIUS™ FLASH-Seq Is Retained Even In Highly Heterogeneous Populations
Number of genes detected in human PBMCs, processed with different protocols, and the number of reads downsampled to 125,000 raw reads.
MERCURIUS™ FLASH-Seq Is A Full-Length ScRNA-Seq Protocol
Gene body coverage shows a uniform read distribution across the entire gene body for the FLASH-seq protocol.
MERCURIUS™ FLASH-Seq Can Discriminate Rare Cell Populations In Heterogeneous Samples
Sample Report
HEK293
192
250'000 reads
10 k
Sample Datasets
PBMCs
96
10'000 reads
To have access to the deep-sequenced dataset (9.5M reads per sample) contact us.
79.9 MB
HEK293
191
250'000 reads
4.9 GiB
Publications
- FLASH-seq, Single-cell FLASH-seq
Andrea Salati, Yves Paychère, Vincent Hahaut, Cédric Gobet, Felix Naef
- FLASH-seq, Single-cell FLASH-seq
Tarek Elmzzahi, Chun-Hsi Su, Mehrnoush Hadaddzadeh Shakiba, Doaa Hamada, DaryaMalko, Maren Koehne, Aleksej Frolov, Teisha Mason, Yuanfang Li, Rebekka Scholz, Collins Osei-Sarpong, Leonie Heyden, Jonas Schulte-Schrepping, Lorenzo Bonaguro, Kristian Haendler, Vassiliki Boussiotis, Annett Halle, Elena De Domenico, Daniel HDGray, Martin Fuhrmann, Zeinab Abdullah, Axel Kallies, Kevin Man, Marc D. Beyer.
- FLASH-seq, Single-cell FLASH-seq
Akisawa Satomi, Riho Saito, Tadahaya Mizuno, Hiroki Sugishita, Hideki Ukai, Shigeyuki Shichino, Masashi Yanagisawa, Kouji Matsushima, Yukiko Gotoh, Tomohiko Okazaki.
What is MERCURIUS™ Single-Cell FLASH-seq, and how does it work?
How is MERCURIUS™ Single-Cell FLASH-seq different to the published FLASH-seq protocol?
We have significantly improved the original FLASH-seq method to offer a streamlined workflow and superior data output. This plate-based technology (available in 96- and 384-well formats) features a novel, non-toxic tagmentation buffer and delivers ultra-sensitive gene detection, capturing up to two times more genes compared to other commercially available solutions.
What type of samples can be used in MERCURIUS™ Single-Cell FLASH-seq?
The MERCURIUS™ FLASH-seq protocol is fully compatible with whole FACS-sorted cells.
We do not recommend very large cells, such as cardiomyocytes, as they are not compatible with the FACS sorting step.
How should I prepare the cells?
We require the cells to be sorted directly into the center of each well in the dedicated 96- and 384-plates, which contain the optimized cell lysis buffer. It is essential for the user to deposit cells in the middle of the well. Please refer to the Sample Submission Guidelines for more details.
Please note! To preserve sample quality during shipping, plates must be placed between dry ice, not just on top. Inadequate cooling may compromise RNA integrity.
What is the turnaround time?
From the moment we receive the frozen plates until the moment we deliver the sequencing data, we typically take four weeks.
Ask your question:
Getting started with our MERCURIUS™ Single-Cell FLASH-seq services is simple
You can either book a call with our experts to discuss your project or submit your experimental details via our contact form so we can review your design and requirements.
Based on the goals, sample types, and scale of your study, we may recommend starting with a pilot project to optimise conditions and de-risk a larger screen.
If you’re interested in implementing the technology in your own lab instead, you can explore our MERCURIUS™ Single-Cell FLASH-seq kits on the dedicated kits page.
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