What is Bulk RNA-seq?

Bulk RNA-seq: A Gateway to Comprehensive Transcriptomics

In the rapidly advancing world of genomics, the ability to analyze gene expression at scale has significantly transformed our understanding of biology and disease. Among the various powerful tools available, bulk RNA sequencing (bulk RNA-seq) stands out as a foundational technique in transcriptomics. But what exactly is bulk RNA-seq, and why is it so crucial in the field of genomics?

What is Bulk RNA-seq?

Bulk RNA-seq is a method that allows researchers to measure the expression levels of thousands of genes simultaneously in a sample containing a mixture of cells. Unlike single-cell RNA sequencing, which focuses on the gene expression of individual cells, bulk RNA-seq provides an averaged expression profile from a collection of cells. This approach is especially useful for studying tissues, 3D organoids, or any samples where analyzing individual cells is not feasible or necessary.

Applications of Bulk RNA-seq

Bulk RNA-seq has become indispensable in various research fields. Some key applications include:

• Differential Gene Expression Analysis

• Biomarker Discovery

• Characterizing Complex Tissues

• Pathway Analysis

By examining the expression of genes within specific biological pathways, researchers can gain insights into molecular mechanisms driving various biological processes and diseases.

Alithea Genomics: Empowering Your Bulk RNA-seq Studies

At Alithea Genomics, we recognize the critical role that bulk RNA-seq plays in advancing research. Our cutting-edge technologies are designed to revolutionize the field of transcriptomics by enabling genome-wide expression analyses of thousands of samples simultaneously, efficiently, and cost-effectively.

The core principle of our technology lies in using highly optimized sets of barcoded primers, which "tag" individual RNA samples in a unique and sample-specific manner. Our oligos are optimized and validated to generate consistent, reproducible, and uniform RNA sequencing data. Currently, up to 384 samples can be pooled and processed together in a single tube.

All our products and services share a common mission: to enable the simple, fast, and cost-efficient generation of "big RNA data" from diverse sample types, including purified RNA, cell and organoid lysates, blood RNA, and plant RNA.

To find out more about our technologies, get in touch with us.