Blood BRB-seq enables industrial and academic clients to generate scalable RNA-seq data directly from whole blood with integrated globin depletion for maximum gene detection.
Discover Sample Datasets
Human
The MERCURIUS ™ Blood BRB-seq service offers a convenient and scalable solution for blood transcriptomics projects from human samples. We are experts in RNA purification from blood samples, including high-throughput extraction from PaxGene tubes and others.
Customers can therefore either send us their stored human blood samples directly or send purified RNA after in-house extraction. Upon receipt of the sample, we always perform incoming quality control before launching our Blood BRBseq pipeline.
Our team then prepares the libraries and sequences to your desired read depth per sample and performs data preprocessing.
We return results, including raw fastq files, sequencing and alignment reports, and gene count matrices suitable for downstream differential expression analyses, once the data meet our rigorous quality control criteria.
During the process, we always keep clients informed at defined checkpoints so we can decide together how best to proceed to the next steps.
For human blood samples, we offer high-throughput RNA extraction directly from PAXgene®, Tempus™ Blood RNA and RNA shield tubes.
Ask for our sample submission guidelines.
(Nanodrop and Fragment analyzer)
1 week
2 days
(Qubit and Fragment analyzer)
1 week
1 week
1 week
Raw FASTQ files, sample report file, QC files, and gene count tables
Distribution of the number of detected genes across 96 blood RNA samples prepared with the MERCURIUS™ Blood BRB-seq library preparation kit. The library was sequenced at an average of 1 million reads per sample on an Illumina NextSeq 550.
Correlation plots between mapped reads in between non-depleted libraries and Blood BRB-seq (left) and Globin Clear-depleted libraries (right). For both methods, globin depletion is effective and specific to the target globin genes, with negligible off-target effects on other genes.
(left) Read distribution to the main globin genes on BRB-seq libraries WITHOUT globin depletion. The majority of mapped genes are associated with globin genes. (right) Read distribution to the main globin genes on BRB-seq libraries WITH globin depletion. The reads associated with globin genes are drastically reduced.
To access the deep-sequenced dataset (4.8 M reads per sample), contact us.
To ensure high-quality data, we typically request that each sample contain at least 12 ng/μl of total RNA in at least 15 μl.
We also offer high-throughput RNA extraction directly from PAXgene®, Tempus™ Blood RNA, and RNA shield tubes.
No, the Blood BRB-seq workflow is only dedicated to human blood samples.
In addition to total RNA amount, it is important that the samples contain RNA of high integrity (RIN > =6) and are devoid of contaminants (Nanodrop A260/A230 between 1.8 and 2.2).
Blood BRB-seq is a 3’-end RNA sequencing method and, as such, requires significantly less sequencing than standard full-length RNA-seq to achieve accurate gene quantification. We therefore normally recommend sequencing 4 to 5 million reads for each sample, which enables the reliable and unbiased detection of over 18,000 genes.
As part of our standard service pipeline, we align the generated data to the genome of choice, provide a detailed report on the alignment and gene counting statistics and, finally, provide ready-to-use gene count matrices for downstream analysis.
You can either book a call with our experts to discuss your project or submit your experimental details via our contact form so we can review your design and requirements.
Based on the goals, sample types, and scale of your study, we may recommend starting with a pilot project to optimise conditions and de-risk a larger screen.
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