MERCURIUS™

Full-Length BRB-seq kits (BETA)

Multiplexed full-length library preparation kits for Illumina® and AVITI™

Up-to 96 full-length RNA-seq library preps in a single tube.


Catalog number	#10801	#11061
Total preps How many library preps can be prepared in total with one kit	96	384
Sample multiplexing and plate format How many samples can be multiplexed in one single tube	96	96
Barcoded oligo-dT plates included	1	4
UDI pairs included How many individual libraries can be sequenced together	4	4
Documentation	User guide Barcode files

Cat ##10801
Total reactions How many library preps can be prepared in total with one kit	96
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Cat ##11061
Total reactions How many library preps can be prepared in total with one kit	384
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Benefits

The MERCURIUS™ Full-Length BRB-seq library preparation kits for Illumina® and AVITI™ contain all the oligos and enzymes needed to go from purified RNA to sequencing-ready libraries.

Multiplexed full-length mRNA-seq: Full-length mRNA-seq library prep, 96 samples in a single tube.

Full-length transcript coverage: From differential gene expression to splicing variants and isoform detection.

One-day lab workflow: Convenient and short protocol from samples to sequencing-ready libraries in one day.

Experimental workflow at a glance
Performance
Product specifications

FL BRB-seq-1

1. Uniform detection of 22'000+ genes at 12 million reads per sample across 48 samples

Distribution of the number of detected genes across 48 samples prepared with the MERCURIUS™ Full-Length BRB-seq service. The library was sequenced at an average of 12 million reads.

2. Full-Length BRB-seq shows high demultiplexing rate and high mapping rate to exons

Full-Length BRB-seq performance shows 99% demultiplexing rate from raw data, 78% mapping rate, and 18% duplication rate of the 48 pooled samples and sequenced at 12 million reads per sample.

3. Mapping rates as high as 80% for different RNA inputs

Mapping rates are uniform across three RNA inputs tested: 1ug, 10ng, and 100ng. Human Universal Reference RNA was used to prepare mRNA Full-Length BRB libraries.

4. Full-Length BRB-seq exhibits reads distribution across the transcript’s entire length

The gene body coverage shows a consistent and uniform reads distribution across the entire gene body for the Full-Length BRB-seq protocol, comparable to competitor N protocol, while the BRB-seq protocol shows a significant 3' bias due to its poly-A selection methodology.

5. >50'000 isoforms detected at 15M sequencing depth

Asset 1@300x

Benchmarking the Full-Length BRB-seq protocol against competitor N shows similar performance for isoform detection. More than 50,000 isoforms can be detected at a sequencing depth of 12M reads per sample.

For (application)	Full-Length mRNA sequencing
For use with (equipment)	Illumina and AVITI NGS instruments
Species compatibility	All eukaryotic species
Available formats	96 and 384 preps
Shipping conditions	Dry ice
Storage conditions	-20C

1. Uniform detection of 22'000+ genes at 12 million reads per sample across 48 samples

Distribution of the number of detected genes across 48 samples prepared with the MERCURIUS™ Full-Length BRB-seq service. The library was sequenced at an average of 12 million reads.

2. Full-Length BRB-seq shows high demultiplexing rate and high mapping rate to exons

Full-Length BRB-seq performance shows 99% demultiplexing rate from raw data, 78% mapping rate, and 18% duplication rate of the 48 pooled samples and sequenced at 12 million reads per sample.

3. Mapping rates as high as 80% for different RNA inputs

Mapping rates are uniform across three RNA inputs tested: 1ug, 10ng, and 100ng. Human Universal Reference RNA was used to prepare mRNA Full-Length BRB libraries.

4. Full-Length BRB-seq exhibits reads distribution across the transcript’s entire length

The gene body coverage shows a consistent and uniform reads distribution across the entire gene body for the Full-Length BRB-seq protocol, comparable to competitor N protocol, while the BRB-seq protocol shows a significant 3' bias due to its poly-A selection methodology.

5. >50'000 isoforms detected at 15M sequencing depth

Benchmarking the Full-Length BRB-seq protocol against competitor N shows similar performance for isoform detection. More than 50,000 isoforms can be detected at a sequencing depth of 12M reads per sample.

Product specifications

FAQs

The barcode set for your kit is conveniently located on the kit label. Please refer to the label for accurate identification.

For optimal compatibility, ensure that you use the appropriate plate format (e.g., for kits designed for 96 reactions, the 96 well-plate format should be used). This ensures accurate and efficient processing of your samples. If you have any further questions or concerns, please contact our support team for assistance by email or using our live chat tool.

Try our service

Our Full-Length BRB-seq service delivers raw sequencing data (fastq files), gene count matrices, and analysis report files. A cost-efficient option suitable for projects of all sizes.

COMPLEMENTARY SERVICE

Cells and organoids (Extraction-free)

Purified RNA

Accessories

Cells and Organoids (EXTRACTION-FREE)

PURIFIED RNA

Whole Blood RNA

Cells and organoids (Extraction-free)

Purified RNA

Accessories

Cells and Organoids (EXTRACTION-FREE)

PURIFIED RNA

Whole Blood RNA

Full-Length BRB-seq kits (BETA)

Accessories

UDI expansion module

Benefits

Sample dataset

Human

FAQs

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