MERCURIUS™

Full-Length Plant BRB-seq service

Full-length mRNA-seq combined with massive sample multiplexing.

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Benefits

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Multiplexed full-length mRNA-seq

Full-length mRNA-seq library prep, 96 samples in a single tube.
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Dedicated service for plant samples

All the reagents used are validated and QC'ed on plant RNA
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Full-length transcript coverage
From differential gene expression to splicing variants and isoform detection.
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Fast turnaround times

From samples to data in as little as six weeks.
MERCURIUSTM

Full-Length Plant BRB-seq service


The MERCURIUS™ Full-Length Plant BRB-seq service offers a convenient and streamlined solution for full-length mRNA transcriptomics projects of any size.

Clients can send us purified plant RNA samples, which are always quality-checked before launching our Full-Length BRB-seq pipeline. During the process, we always keep clients informed at defined checkpoints so that we can decide together how to best proceed to the next steps.

Next generation sequencing and data pre-processing (including alignment to the genome of choice) are part of our standard service as well. As a result, we provide our clients raw data, sequencing and alignment reports, and gene count matrices which can be used for downstream gene expression analysis.

Optional downstream differential gene expression analysis are also available upon request.

BRB-seq service in action

How it works

Step 1

Step 3

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Client ships samples to Alithea

Step 4

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Incoming QC - Client Checkpoint

(Nanodrop and Fragment Analyzer)

time 1 week

Step 5

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MERCURIUS™ Full-Length BRB-seq library preparation

 

time 2 days

Step 6

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Library QC - Client Checkpoint

(Qubit, Fragment analyzer, shallow sequencing)

time 1 week

Step 7

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Deep sequencing

 

 

time 1 week

Step 8

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Data analysis and reporting - Client Checkpoint

Step 9

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Data

Optional: Alithea performs Differential Gene Expression analysis​

Performance

Gene detection pool

Uniform detection of 22'000+ genes at 12 million reads per sample across 48 samples

Distribution of the number of detected genes across 48 samples prepared with the MERCURIUS™ Full-Length BRB-seq service. The library was sequenced at an average of 12 million reads.

Performance FL BRB-seq

Full-Length BRB-seq shows high demultiplexing rate and high mapping rate to exons

Full-Length BRB-seq performance shows 99% demultiplexing rate from raw data, 78% mapping rate, and 18% duplication rate of the 48 pooled samples and sequenced at 12 million reads per sample.

Mapping rate

Mapping rates as high as 80% for different RNA inputs

Mapping rates are uniform across three RNA inputs tested: 1ug, 10ng, and 100ng. Human Universal Reference RNA was used to prepare mRNA Full-Length BRB libraries. 

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Full-Length BRB-seq exhibits reads distribution across the transcript’s entire length

The gene body coverage shows a consistent and uniform reads distribution across the entire gene body for the Full-Length BRB-seq protocol, comparable to the NEB protocol, while the BRB-seq protocol  shows a significant 3' bias due to its poly-A selection methodology.

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>50'000 isoforms detected at 12M sequencing depth

Benchmarking the Full-Length BRB-seq protocol against the competitor NEB shows similar performance for isoform detection. More than 50,000 isoforms can be detected at a sequencing depth of 12M reads per sample.

FAQs

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Relevant publications

Explore the latest, relevant publications in the industry to learn more about BRB-seq.

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Want to do Full-Length Plant BRB-seq in-house?

Our Full-Length Plant BRB-seq kits combine the full-length mRNA-seq with massive sample multiplexing while providing the most cost-effective solution.
Experience sample to sequencing-ready library in one day.

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Book a one-on-one call with one of our RNA experts to discover how we can assist your next project.

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