MERCURIUS™

Blood BRB-seq kits

Multiplexed whole blood RNA library preparation kits for Illumina^® and AVITI™

96, 384 RNA-seq library preps in one tube with inline globin depletion.


Catalog number	#10823	#11023	#10825	#11025
Total preps How many library preps can be prepared in total with one kit	96	384	384	1,536
Sample multiplexing and plate format How many samples can be multiplexed in one single tube	96	96	384	384
Barcoded oligo-dT plates included	1	4	1	4
UDI pairs included How many individual libraries can be sequenced together	4	4	4	4
Globin blockers	included	included	included	included
Documentation	User guide Barcode files

Cat ##10823
Total reactions How many library preps can be prepared in total with one kit	96
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4
Globin blockers	included
Download Manual

Cat ##11023
Total reactions How many library preps can be prepared in total with one kit	384
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4
Globin blockers	included
Download Manual

Cat ##10825
Total reactions How many library preps can be prepared in total with one kit	384
RNA multiplexing format How many library preps can be prepared in total with one kit	384
UDI pairs included How many library preps can be prepared in total with one kit	4
Globin blockers	included
Download Manual

Cat ##11025
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit	384
UDI pairs included How many library preps can be prepared in total with one kit	4
Globin blockers	included
Download Manual

Benefits

The  MERCURIUS™ Blood BRB-seq library preparation kits for Illumina^®and AVITI™ contain all the oligos and enzymes needed to go from purified blood RNA to sequencing-ready libraries.

Bulk RNA sequencing at scale: More samples, more replicates. Robust results, significant discoveries.

Inline globin depletion: Seamlessly integrated globin depletion. No need to purchase additional kits.

One-day lab workflow: Convenient and short protocol from samples to sequencing-ready libraries in one day.

Experimental workflow at a glance
Performance
Product specifications

Blood BRB-seq-2-1

1. Uniform detection of 15'000+ genes at 1 million reads per sample across 96 samples

Distribution of the number of detected genes across 96 blood RNA samples prepared with the MERCURIUS™ Blood BRB-seq library preparation kit. The library was sequenced at an average of 1 million reads per sample on an Illumina NextSeq 550.

2. The Blood BRB-seq provides state-of-the-art performance in terms of globin depletion efficiency

(left) Proportion of mapped reads assigned to globin genes (blue) and other genes (grey) for blood RNA samples without globin depletion and with globin depletion performed using ThermoFisher’s GLOBINclear™-Human Kit and the Blood BRB-seq method. (right) Number of detected genes across the three different conditions. Non-depleted libraries show a sharp decrease in performance, while BRB-seq and Globin Clear libraries both provide rich globin-free data.

3. Globin depletion is effective and specific to the target globin genes

Picture1-Nov-24-2023-11-21-07-7672-AM

Correlation plots between mapped reads in between non-depleted libraries and Blood BRB-seq (left) and Globin Clear-depleted libraries (right). For both methods, globin depletion is effective and specific to the target globin genes, with negligible off-target effects on other genes.

4. Blood BRB-seq provides effective and high-throughput inline depletion of globin genes

(left) Read distribution to the main globin genes on BRB-seq libraries WITHOUT globin depletion. The majority of mapped genes are associated with globin genes. (right) Read distribution to the main globin genes on BRB-seq libraries WITH globin depletion. The reads associated to globin genes are drastically reduced.

For (application)	3' mRNA sequencing
For use with (equipment)	Illumina and AVITI NGS instruments
Species compatibility	Human and mouse
Available formats	96, 384 and 1'536 preps
Shipping conditions	Dry ice
Storage conditions	-20C

1. Uniform detection of 15'000+ genes at 1 million reads per sample across 96 samples

Distribution of the number of detected genes across 96 blood RNA samples prepared with the MERCURIUS™ Blood BRB-seq library preparation kit. The library was sequenced at an average of 1 million reads per sample on an Illumina NextSeq 550.

2. The Blood BRB-seq provides state-of-the-art performance in terms of globin depletion efficiency

(left) Proportion of mapped reads assigned to globin genes (blue) and other genes (grey) for blood RNA samples without globin depletion and with globin depletion performed using ThermoFisher’s GLOBINclear™-Human Kit and the Blood BRB-seq method. (right) Number of detected genes across the three different conditions. Non-depleted libraries show a sharp decrease in performance, while BRB-seq and Globin Clear libraries both provide rich globin-free data.

3. Globin depletion is effective and specific to the target globin genes

Correlation plots between mapped reads in between non-depleted libraries and Blood BRB-seq (left) and Globin Clear-depleted libraries (right). For both methods, globin depletion is effective and specific to the target globin genes, with negligible off-target effects on other genes.

4. Blood BRB-seq provides effective and high-throughput inline depletion of globin genes

(left) Read distribution to the main globin genes on BRB-seq libraries WITHOUT globin depletion. The majority of mapped genes are associated with globin genes. (right) Read distribution to the main globin genes on BRB-seq libraries WITH globin depletion. The reads associated to globin genes are drastically reduced.

Product specifications

Sample dataset

Human

Number of samples:
96

Reads per sample in demo dataset:
10'000 reads

To have access to the deep-sequenced dataset (4.8M reads per sample) contact us.

Demo dataset file size:
57.7 MB

Download

FAQs

Each BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.

To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
One of the key advantages of BRB-seq is that it does not only save reagents and cost in the library preparation stage, but also in the sequencing one.

As opposed to standard RNA-seq, where 20M-30M reads per sample are required, we normally recommend to sequence BRB-seq libraries at a depth of 4M-5M reads per sample, which is normally enough to detect the vast majority of expressed genes.
The barcode set for your kit is conveniently located on the kit label. Please refer to the label for accurate identification.

For optimal compatibility, ensure that you use the appropriate plate format (e.g., for kits designed for 96 reactions, the 96 well-plate format should be used). This ensures accurate and efficient processing of your samples. If you have any further questions or concerns, please contact our support team for assistance by email or using our live chat tool.

Relevant publications

Explore the latest, relevant publications in the industry to learn more about our technologies.

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Our Blood BRB-seq service deliver raw sequencing data (fastq files), gene count matrices and analysis report files. A cost-efficient option suitable for projects of all sizes.

Cells and organoids (Extraction-free)

Purified RNA

Accessories

Cells and Organoids (EXTRACTION-FREE)

PURIFIED RNA

Whole Blood RNA

Cells and organoids (Extraction-free)

Purified RNA

Accessories

Cells and Organoids (EXTRACTION-FREE)

PURIFIED RNA

Whole Blood RNA

Blood BRB-seq kits

Accessories

UDI expansion module

Benefits

Sample dataset

Human

FAQs

Relevant publications

Try our service

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