MERCURIUS™

Cell Lysate BRB-seq
Extraction-free library preparation kits for Illumina®

RNA-seq library preps directly from cell lysates without prior RNA isolation.

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Cat #10840 CP_AG_FrontTall_Comp_Transparent_Small
Total reactions How many library preps can be prepared in total with one kit 24
RNA multiplexing format How many library preps can be prepared in total with one kit 24
UDI pairs included How many library preps can be prepared in total with one kit 1
icn_blood-brb-seq
Cat #10841 CP_AG_FrontTall_Comp_Transparent_Small
Total reactions How many library preps can be prepared in total with one kit 96
RNA multiplexing format How many library preps can be prepared in total with one kit 96
UDI pairs included How many library preps can be prepared in total with one kit 4
icn_blood-brb-seq
Cat #11041 CP_AG_FrontTall_Comp__Small
Total reactions How many library preps can be prepared in total with one kit 384
RNA multiplexing format How many library preps can be prepared in total with one kit 96
UDI pairs included How many library preps can be prepared in total with one kit 4
icn_blood-brb-seq
Cat #10851 CP_AG_FrontTall_Comp_Transparent_Small
Total reactions How many library preps can be prepared in total with one kit 384
RNA multiplexing format How many library preps can be prepared in total with one kit 384
UDI pairs included How many library preps can be prepared in total with one kit 4
icn_blood-brb-seq
Cat #11051 CP_AG_FrontTall_Comp__Small
Total reactions How many library preps can be prepared in total with one kit 1536
RNA multiplexing format How many library preps can be prepared in total with one kit 384
UDI pairs included How many library preps can be prepared in total with one kit 4
icn_blood-brb-seq

Kit Pricing

Discover the unique scalability offered by BRB-seq

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Custom Format

For more reactions, higher multiplexing and more UDIs

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Why choose our MERCURIUS™ Cell Lysate BRB-seq library preparation kits for Illumina®

The Extraction-free Cell Lysate BRB-seq kits contain all the oligos and enzymes needed to go from 2D cell cultures and organoids to sequencing-ready DNA libraries.

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Ideal for screening projects

More samples, more replicates. Robust results, significant discoveries.

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Streamlined data pre-processing

Demultiplex and align your BRB-seq data with our easy-to-use cloud-based platform.

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No need for prior RNA extraction

An optimized lysis buffer for complete lysis and efficient reverse transcription.

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One-day lab workflow

Convenient and short protocol from samples to sequencing-ready libraries in one day.

The generated 3' mRNA-seq data is ideally suited for differential gene expression analysis in the screening setting.

As compared to other bulk 3' mRNA-seq solutions, BRB-seq makes it possible to process virtually any number of cell line samples in one single tube, which helps drastically reduce technical variation, turnaround times and costs. 

The particular aspect of the Cell Lysate BRB-seq workflow is the addition of proprietary lysis buffer, which enables complete lysis of the cellular material while still being compatible with downstream enzymatic reactions.

Each kit contains enough reagents (including Unique Dual Indexing oligos) to prepare up-to four sequencing libraries.

After sequencing, the generated data can be easily demultiplexed and aligned to the genome of choice using our dedicated cloud-based pipeline, which does not require prior bioinformatic experience.

Experimental workflow at a glance

Product Specifications

For use with (application)
3' mRNA sequencing
For use with (equipment)
Illumina NGS instruments
Validated species
Human and mouse cell lines. Organoids.
Species compatibility
All eukaryotic species
Available formats
24, 96, 384, 1536 reactions
Shipping conditions
Dry ice
Storage conditions
-20C

FAQs

  • Each BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.

    To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.

  • The recommended range of input material is in the range of 5’000-50’000 cells.
  • The only difference between BRB-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the BRB-seq barcode sequence.

    For a thorough description of BRB-seq data processing, please refer to the BRB-seq kit user guide. In order to facilate as much as possible BRB-seq data analysis, we have also created a dedicate pipeline which can be found in our “SOFTWARE” page.

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Our BRB-seq service delivers raw sequencing data (fastq files), gene count matrices and analysis report files. A cost-efficient option suitable for projects of all sizes.

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