|Cat #10941||Cat #11141|
|Total reactions How many library preps can be prepared in total with one kit||96||384|
|RNA multiplexing format How many samples can be multiplexed in one single tube||96||96|
The MERCURIUS™ Extraction-free DRUG-seq kits contain all the oligos and enzymes needed to go from 2D cell cultures to sequencing-ready DNA libraries.
No additional library conversion kit is required!
No need for prior RNA extraction
An optimized lysis buffer for complete lysis and efficient reverse transcription.
Improved DRUG-seq protocol
Without pre-amplification, leading to higher mapping and gene detection rates.
Ideal for screening projects
More samples, more replicates. Robust results, significant discoveries.
Specially designed for MGI systems, no additional library conversion kit is needed.
One-day lab workflow
Convenient and short protocol from samples to sequencing-ready libraries in one day.
Experimental workflow at a glance
Each BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.
To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
The recommended range of input material is in the range of 5’000-50’000 cells.
The only difference between BRB-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the BRB-seq barcode sequence.
For a thorough description of BRB-seq data processing, please refer to the BRB-seq kit user guide. In order to facilate as much as possible BRB-seq data analysis, we have also created a dedicate pipeline which can be found in our “SOFTWARE” page.
Speak with our RNA sequencing experts
Book a one-on-one call with one of our RNA experts to discover how we can assist your next project.