MERCURIUS™
DRUG-seq
Multiplexed extraction-free library preparation kits for MGI
RNA-seq library preps directly from cell lysates without prior RNA isolation.
Catalog number | #10941 | #11141 | |
Total preps How many library preps can be prepared in total with one kit | 96 | 384 | |
RNA and plate multiplexing format How many samples can be multiplexed in one single tube | 96 | 96 | |
Barcoded oligo-dT plates included | 1 | 4 | |
Fragmentation adaptors | 4 | 4 | |
Documentation |
Cat#10941 | ||||
---|---|---|---|---|
Total reactions How many library preps can be prepared in total with one kit | ||||
RNA multiplexing format How many library preps can be prepared in total with one kit | 96 | |||
UDI pairs included How many library preps can be prepared in total with one kit | 4 | |||
Cat#11141 | ||||
---|---|---|---|---|
Total reactions How many library preps can be prepared in total with one kit | ||||
RNA multiplexing format How many library preps can be prepared in total with one kit | 96 | |||
UDI pairs included How many library preps can be prepared in total with one kit | 4 | |||
-
Benefits
The MERCURIUS™ Extraction-free DRUG-seq kits contain all the oligos and enzymes needed to go from 2D cell cultures to sequencing-ready DNA libraries. No additional library conversion kit is required!
No need for prior RNA extractionImproved DRUG-seq protocolIdeal for screening projectsReady-to-uselibraryOne-day labworkflowAn optimized lysis buffer for complete lysis and efficient reverse transcription.
Without amplification, leading to higher mapping and gene detection rates.
More samples, more replicates. Robust results, significant discoveries.
Specially designed for MGI systems, no additional library conversion kit is needed.
Convenient and short protocol. From samples to sequencing-ready libraries in one day.
-
Experimental workflow at a glance
-
Product specifications
For (application)
3' mRNA sequencing
For use with (equipment)
MGI T and G series instruments
Species compatibility
All eukaryotic species
Available formats
96, 384 reactions
Shipping conditions
Dry ice
Storage conditions
-20C
FAQs
-
Each BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.
To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
-
The recommended range of input material is in the range of 5’000-50’000 cells.
-
The only difference between DRUG-seq and standard RNA-seq data analysis is the demultiplexing step, which assigns sequencing reads to their origin sample based on the DRUG-seq barcode sequence.
For a thorough description of DRUG-seq data processing, please refer to the DRUG-seq kit user guide.
Speak with our RNA sequencing experts
Book a one-on-one call with one of our RNA experts to discover how we can assist your next project.