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Total reactions
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96 | 384 | |||
RNA multiplexing format
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96 | 96 | |||
Fragmentation Adaptors | 4 | 4 | |||
Data analysis | |||||
Manual |
Benefits
The MERCURIUS™ Extraction-free DRUG-seq kits contain all the oligos and enzymes needed to go from 2D cell cultures to sequencing-ready DNA libraries.
No additional library conversion kit is required!
No need for prior RNA extraction
An optimized lysis buffer for complete lysis and efficient reverse transcription.
Improved DRUG-seq protocol
Without pre-amplification, leading to higher mapping and gene detection rates.
Ideal for screening projects
More samples, more replicates. Robust results, significant discoveries.

Ready-to-use library
Specially designed for MGI systems, no additional library conversion kit is needed.
One-day lab workflow
Convenient and short protocol from samples to sequencing-ready libraries in one day.
Experimental workflow at a glance

Product Specifications
FAQs
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Each BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.
To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
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The recommended range of input material is in the range of 5’000-50’000 cells.
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The only difference between BRB-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the BRB-seq barcode sequence.
For a thorough description of BRB-seq data processing, please refer to the BRB-seq kit user guide. In order to facilate as much as possible BRB-seq data analysis, we have also created a dedicate pipeline which can be found in our “SOFTWARE” page.
Speak with our RNA sequencing experts
Book a one-on-one call with one of our RNA experts to discover how we can assist your next project.