MERCURIUS™

DRUG-seq kits

Multiplexed extraction-free library preparation kits for Illumina®, AVITI™ and MGI*

RNA-seq library preps directly from cells without prior RNA isolation.

* For native kits' compatibility with MGI sequencers, contact us.


Catalog number	#10841	#11041	#10851	#11051
Total preps How many library preps can be prepared in total with one kit	96	384	384	1,536
Sample multiplexing and plate format How many samples can be multiplexed in one single tube	96	96	384	384
Barcoded oligo-dT plates included	1	4	1	4
UDI pairs included How many individual libraries can be sequenced together	4	4	4	4
Documentation	User guide Barcode files

Cat ##10841
Total reactions How many library preps can be prepared in total with one kit	96
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Cat ##11041
Total reactions How many library preps can be prepared in total with one kit	384
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Cat ##10851
Total reactions How many library preps can be prepared in total with one kit	384
RNA multiplexing format How many library preps can be prepared in total with one kit	384
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Cat ##11051
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit	384
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Accessories

Cell lysis module

Optimized lysis buffer for complete lysis and efficient reverse transcription.
Compatible with DRUG-seq library prep kits.

Find out more

UDI expansion module

Compatible with the MERCURIUS™ product line.
12 UDI pairs.
Flexible dual indexing solutions.

Find out more

Benefits

The MERCURIUS™ Extraction-free DRUG-seq kits contain all the oligos and enzymes needed to go from 2D cell cultures to sequencing-ready libraries.

Ideal for screening projects: More samples, more replicates. Robust results, significant discoveries.

No need for prior RNA extraction: An optimized lysis buffer for complete lysis and efficient reverse transcription.

Improved protocol: Without pre-amplification, leading to higher mapping and gene detection rates.

One-day lab workflow: Convenient and short protocol from samples to sequencing-ready libraries in one day.

Experimental workflow at a glance
Performance
Product specifications
Validated cell lines

DRUG-seq-2-1

1. Detection of 12'000+ genes at 1.5 million reads per sample across 384 samples

Distribution of the number of detected genes across 384 samples prepared with the MERCURIUS™ DRUG-seq library preparation kit from frozen cells. The library was sequenced at an average of 1.5 million reads per sample on an Illumina NovaSeq 6000.

2. DRUG-seq shows high demultiplexing rate and high mapping rate to exons

DRUG-seq performance shows 97% demultiplexing rate from raw data and 85% mapping rate of the 384 pooled samples, sequenced at 1.5 million reads per sample.

3. DRUG-seq shows a comparable number of detected genes at different cell seeding densities

No of detected genes per no cells per well_1@300x

The number of detected genes was assessed for three different numbers of cells per well: 1k, 5k, and 100k cells per well. The libraries were then sequenced at 1.5 million reads per sample.

4. Comparable number of detected genes between the DRUG-seq and BRB-seq protocol

The number of detected genes was assessed between the DRUG-seq protocol, RNA extraction-free: directly from frozen cell lysates, and the BRB-seq protocol, from purified RNA. Each library contains 24 samples, sequenced at aprox 2M reads/sample.

5. Library performance is comparable between the DRUG-seq and BRB-seq protocol

Both libraries, DRUG-seq from frozen cell lysates, and BRB-seq from purified RNA, show a very high demultiplexing rate, above 99% and a mapping rate above 76%.

For (application)	3' mRNA sequencing
For use with (equipment)	Illumina and AVITI NGS instruments
Species compatibility	All eukaryotic species
Available formats	96, 384 preps 1'536 preps
Shipping conditions	Dry ice
Storage conditions	-20C

Cell line	Species	Tissue	Culture type
A375	h.sapiens	Skin, malignant melanoma	adherent
GM12878	h.sapiens	PBMC, lymphoblastoid	suspension
H295R	h.sapiens	Adrenal gland, carcinoma	adherent
HAP1	h.sapiens	KBM-7 derived, chronic myelogenous leukemia	adherent
hASC	h.sapiens	Patient-derived adipose stromal cells	adherent
hASC-Adipocytes	h.sapiens	Differentiated hASC	adherent
HEK293	h.sapiens	Kidney embryonic	adherent
HeLa	h.sapiens	Cervix, adenocarcinoma	adherent
HepG2	h.sapiens	Liver, carcinoma	adherent
Huh7	h.sapiens	Liver, carcinoma	adherent
iNeurons	h.sapiens	Differentiated iPSC	adherent
MCF-7	h.sapiens	Breast, adenocarcinoma	adherent
PANC-1	h.sapiens	Pancreas, carcinoma	adherent
U2OS	h.sapiens	Bone, osteosarcoma	adherent

1. Detection of 12'000+ genes at 1.5 million reads per sample across 384 samples

Distribution of the number of detected genes across 384 samples prepared with the MERCURIUS™ DRUG-seq library preparation kit from frozen cells. The library was sequenced at an average of 1.5 million reads per sample on an Illumina NovaSeq 6000.

2. DRUG-seq shows high demultiplexing rate and high mapping rate to exons

DRUG-seq performance shows 97% demultiplexing rate from raw data and 85% mapping rate of the 384 pooled samples, sequenced at 1.5 million reads per sample.

3. DRUG-seq shows a comparable number of detected genes at different cell seeding densities

The number of detected genes was assessed for three different numbers of cells per well: 1k, 5k, and 100k cells per well. The libraries were then sequenced at 1.5 million reads per sample.

4. Comparable number of detected genes between the DRUG-seq and BRB-seq protocol

The number of detected genes was assessed between the DRUG-seq protocol, RNA extraction-free: directly from frozen cell lysates, and the BRB-seq protocol, from purified RNA. Each library contains 24 samples, sequenced at aprox 2M reads/sample.

5. Library performance is comparable between the DRUG-seq and BRB-seq protocol

Both libraries, DRUG-seq from frozen cell lysates, and BRB-seq from purified RNA, show a very high demultiplexing rate, above 99% and a mapping rate above 76%.

Product specifications

Validated cell lines

Sample datasets

Hap1

Number of samples:
24

Reads per sample in demo dataset:
10'000 reads

To have access to the deep-sequenced dataset (7.3 M reads per sample) contact us.

Demo dataset file size:
13.3 MB

Download

HepG2

Number of samples:
65

Reads per sample in demo dataset:
10'000 reads

To have access to the deep-sequenced dataset (3.9 M reads per sample) contact us.

Demo dataset file size:
4.76 MB

Download

HeLa

Number of samples:
24

Reads per sample in demo dataset:
10'000 reads

To have access to the deep-sequenced dataset (7.1 M reads per sample) contact us.

Demo dataset file size:
13.6 MB

Download

FAQs

Each DRUG-seq or BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.

To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
The barcode set for your kit is conveniently located on the kit label. Please refer to the label for accurate identification.

For optimal compatibility, ensure that you use the appropriate plate format (e.g., for kits designed for 96 reactions, the 96 well-plate format should be used). This ensures accurate and efficient processing of your samples. If you have any further questions or concerns, please contact our support team for assistance by email or using our live chat tool.

Relevant publications

Explore the latest, relevant publications in the industry to learn more about our technologies.

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Our DRUG-seq services deliver raw sequencing data (fastq files), gene count matrices and analysis report files. A cost-efficient option suitable for projects of all sizes.

COMPLEMENTARY SERVICE

Cells and organoids (Extraction-free)

Purified RNA

Accessories

Cells and Organoids (EXTRACTION-FREE)

PURIFIED RNA

Whole Blood RNA

Cells and organoids (Extraction-free)

Purified RNA

Accessories

Cells and Organoids (EXTRACTION-FREE)

PURIFIED RNA

Whole Blood RNA

DRUG-seq kits

Accessories

Cell lysis module

UDI expansion module

Benefits

Sample datasets

Hap1

HepG2

HeLa

FAQs

Relevant publications

Try our service

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