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MERCURIUS™

BRB-seq
Library preparation kits
for MGI

 96 RNA-seq library preps in one tube.

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IMG_AG_BRB-seq_Kit_MGI
Cat #10913 CP_AG_FrontTall_Comp_Transparent_Small
Total reactions How many library preps can be prepared in total with one kit 96
RNA multiplexing format How many library preps can be prepared in total with one kit 96
UDI pairs included How many library preps can be prepared in total with one kit 4
icn_blood-brb-seq
Cat #11113 CP_AG_FrontTall_Comp__Small
Total reactions How many library preps can be prepared in total with one kit 384
RNA multiplexing format How many library preps can be prepared in total with one kit 96
UDI pairs included How many library preps can be prepared in total with one kit 4
icn_blood-brb-seq

Kit Pricing

Discover the unique scalability offered by BRB-seq

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Custom Format

For more reactions, higher multiplexing, fragmentation adaptors

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Benefits

The MERCURIUS™ BRB-seq library preparation kits for MGI are ready-to- use!
No additional library conversion kit is required!

Asset 1-Feb-23-2023-07-11-14-0429-PM-1

Ready-to-use library

Specially designed for MGI systems, no additional library conversion kit is needed.

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RNA sequencing at scale

Perform up-to 96 RNA-seq library preparations in one single tube.

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One-day lab workflow

Convenient and short protocol from samples to sequencing-ready libraries in one day.

Experimental workflow at a glance

BRB-seq MGI-1-1

Product Specifications

For use with (application)
3' mRNA sequencing
For use with (equipment)
MGI T and G series instruments
Species compatibility
All eukaryotic species
Available formats
96, 384 reactions
Shipping conditions
Dry ice
Storage conditions
-20C

FAQs

  • Each BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.

    To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.

  • The recommended range of RNA amount for each sample is of 50ng-1μg, normally the more RNA, the better.

    The minimum recommended RIN number is 6 and the A260/230 ratio (Nanodrop) should be in the 1.5-2.2 range.

  • The only difference between BRB-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the BRB-seq barcode sequence.

    For a thorough description of BRB-seq data processing, please refer to the BRB-seq kit user guide. In order to facilate as much as possible BRB-seq data analysis, we have also created a dedicate pipeline which can be found in our “SOFTWARE” page.

  • One of the key advantages of BRB-seq is that it does not only save reagents and cost in the library preparation stage, but also in the sequencing one.

    As opposed to standard RNA-seq, where 20M-30M reads per sample are required, we normally recommend to sequence BRB-seq libraries at a depth of 4M-5M reads per sample, which is normally enough to detect the vast majority of expressed genes.

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Our BRB-seq and DRUG-seq services deliver raw sequencing data (fastq files), gene count matrices and analysis report files. A cost-efficient option suitable for projects of all sizes.

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Speak with our RNA sequencing experts

Book a one-on-one call with one of our RNA experts to discover how we can assist your next project.