MERCURIUS™ BRB-seq kit for MGI


Catalog number	#10913	#11113
Total preps How many library preps can be prepared in total with one kit	96	384
RNA and plate multiplexing format How many samples can be multiplexed in one single tube	96	96
Barcoded oligo-dT plates included	1	4
Fragmentation adaptors	4	4
Documentation	User guide

Cat#10913
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

Cat#11113
Total reactions How many library preps can be prepared in total with one kit
RNA multiplexing format How many library preps can be prepared in total with one kit	96
UDI pairs included How many library preps can be prepared in total with one kit	4
Download Manual

The MERCURIUS™ BRB-seq library preparation kits for MGI are ready-to-use! No additional library conversion kit is required!

Ready-to-use library

Bulk RNA sequencing

at scale

One-day lab

workflow

Specially designed for MGI systems, no additional library conversion kit is needed.

Perform up-to 384 RNA-seq library preparations in one single tube.

Convenient and short protocol from samples to sequencing-ready libraries in one day.

For (application)	3' mRNA sequencing
For use with (equipment)	MGI T and G series instruments
Species compatibility	All eukaryotic species
Available formats	96, 384 reactions
Shipping conditions	Dry ice
Storage conditions	-20C

Each BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.

To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
The recommended range of RNA amount for each sample is of 50ng-1μg, normally the more RNA, the better.

The minimum recommended RIN number is 6 and the A260/230 ratio (Nanodrop) should be in the 1.5-2.2 range.
The only difference between BRB-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the BRB-seq barcode sequence.

For a thorough description of BRB-seq data processing, please refer to the BRB-seq kit user guide.
One of the key advantages of BRB-seq is that it does not only save reagents and cost in the library preparation stage, but also in the sequencing one.

As opposed to standard RNA-seq, where 20M-30M reads per sample are required, we normally recommend to sequence BRB-seq libraries at a depth of 4M-5M reads per sample, which is normally enough to detect the vast majority of expressed genes.

FAQs