MERCURIUS™
BRB-seq
Library preparation kits for MGI
96, 384 RNA-seq library preps in one tube.
Catalog number | #10913 | #11113 | |
Total preps
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96 | 384 | |
RNA and plate multiplexing format
|
96 | 96 | |
Barcoded oligo-dT plates included | 1 | 4 | |
Fragmentation adaptors | 4 | 4 | |
Documentation |
Cat#10913 | ||||
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Total reactions
|
||||
RNA multiplexing format
|
96 | |||
UDI pairs included
|
4 | |||
Cat#11113 | ||||
---|---|---|---|---|
Total reactions
|
||||
RNA multiplexing format
|
96 | |||
UDI pairs included
|
4 | |||
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Benefits
The MERCURIUS™ BRB-seq library preparation kits for MGI are ready-to-use! No additional library conversion kit is required!
Ready-to-use libraryBulk RNA sequencingat scaleOne-day labworkflowSpecially designed for MGI systems, no additional library conversion kit is needed.
Perform up-to 384 RNA-seq library preparations in one single tube.
Convenient and short protocol from samples to sequencing-ready libraries in one day.
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Experimental workflow at a glance
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Product specifications
For (application)
3' mRNA sequencing
For use with (equipment)
MGI T and G series instruments
Species compatibility
All eukaryotic species
Available formats
96, 384 reactions
Shipping conditions
Dry ice
Storage conditions
-20C
FAQs
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Each BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.
To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
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The recommended range of RNA amount for each sample is of 50ng-1μg, normally the more RNA, the better.
The minimum recommended RIN number is 6 and the A260/230 ratio (Nanodrop) should be in the 1.5-2.2 range.
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The only difference between BRB-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the BRB-seq barcode sequence.
For a thorough description of BRB-seq data processing, please refer to the BRB-seq kit user guide.
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One of the key advantages of BRB-seq is that it does not only save reagents and cost in the library preparation stage, but also in the sequencing one.
As opposed to standard RNA-seq, where 20M-30M reads per sample are required, we normally recommend to sequence BRB-seq libraries at a depth of 4M-5M reads per sample, which is normally enough to detect the vast majority of expressed genes.
Speak with our RNA sequencing experts
Book a one-on-one call with one of our RNA experts to discover how we can assist your next project.