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Large-scale RNA-seq made easy

By leveraging BRB-seq, we not only provide to industrial and academic clients high quality RNA-seq data, but we also do so with the highest affordability and shortest turnaround times on the market.

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Benefits

The generated 3' mRNA-seq data is ideally suited for differential gene expression analysis.

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Bulk RNA sequencing at scale

More samples, more replicates. Robust results, significant discoveries.

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Reducing sequencing costs

Genome-wide digital gene expression profiling from as low as 4M reads per sample.

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Ideal for projects of all sizes

You can trust us. We have ran projects from as low as 6 samples to as many as 9,000.

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Fast turnaround times

From samples to data in as little as 1.5 months.

As compared to other mRNA-seq solutions, BRB-seq makes it possible to process virtually any number of RNA samples in one single tube, which helps reduce technical variation, turnaround times and costs.

Each kit contains enough reagents (including Unique Dual Indexing oligos) to prepare up-to three sequencing libraries.

After sequencing, the generated data can be easily demultiplexed and aligned to the genome of choice using our dedicated cloud-based pipeline, which does not require prior bioinformatic experience.

What we do

MERCURIUS™ BRB-seq service

The MERCURIUS™ BRB-seq service offers a convenient and streamlined solution for transcriptomics projects of any size. Clients can send us purified RNA samples, which are always quality-checked before launching our BRB-seq pipeline. During the process, we always keep clients informed at defined checkpoint so that we can decide together how to best proceed to the next steps.

Next generation sequencing and data pre-processing (including alignment to the genome of choice) are part of our standard service as well. As a result, we provide our clients raw data, sequencing and alignment reports, and gene count matrices which can be used for downstream gene expression analysis.

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MERCURIUS™ Blood BRB-seq service

The MERCURIUS™ Blood BRB-seq service offers a convenient and streamlined solution for your blood transcriptomics projects. We have expertise with different types of RNA purification from blood samples and we have specialized in high-throughput RNA extraction from PaxGene tubes.

Customers can therefore either send us directly their blood samples or perform RNA extraction in-house and send us purified RNA samples. Upon sample reception, we always perform incoming QC before launching our Blood BRBseq pipeline. During the process, we always keep clients informed at the defined checkpoints so that we can decide together how to best proceed to the next steps.

Next-generation sequencing and data preprocessing (including alignment to the genome of choice) are part of our standard service as well. As a result, we provide our clients raw data, sequencing and alignment reports, and gene count matrices which can be used for downstream gene expression analysis.

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MERCURIUS™ Cell Lysate BRB-seq service

The MERCURIUS™ Cell Lysate BRB-seq service offers the most comprehensive, high-throughput and cost-efficient solution for gene expression analysis projects starting from 2D cell cultures or organoids.

As part of the Cell Lysate service, we send our clients a proprietary Lysis Buffer which is optimized for both cell lysis and downstream enzymatic reactions, without having to perform RNA extraction in between.

After mixing the Lysis Buffer with their cells, the client can send back to us their cell lysates, which are then ready to be processed in the BRB-seq workflow.

By leveraging on the advantages the Cell Lysate BRB-seq system, such as cost-efficiency and high-throughput, we are able to offer the most competitive price and turnaround time on the market.

As a result of our service, our clients receive raw sequencing data (fastq files), gene count matrices and analysis reports files.

Optional downstream differential gene expression analysis are also available upon request.

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How does it work

  • Client ships samples to Alithea

    For blood samples, we offer high-throughput RNA extraction directly from PAXgene ® and Tempus ™ Blood RNA tubes
    Sample preparation guidelines for purified RNA (PDF) Sample preparation guidelines for cell lysates (PDF)
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  • 1 Week

    Incoming QC - Client Checkpoint

    (Nanodrop, Ribogreen, Tapestation)
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  • 2 Days

    MERCURIUS™ BRB-seq library preparation kits for Illumina®

    MERCURIUS™ Blood BRB-seq library preparation kits for Illumina®

    MERCURIUS™ Extraction-free Cell Lysate BRB-seq kits for Illumina®

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  • 1 week

    Library QC - Client Checkpoint

    (Qubit, Fragment analyzer, shallow sequencing)
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  • 1.5 weeks

    Deep sequencing - Illumina

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  • 1 week

    Data analysis and reporting - Client Checkpoint

    Download sample report
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  • Data

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FAQs

  • To guarantee high quality data, we normally request that each sample contains at least 200ng of total RNA in at least 10μl.
  • In addition to total RNA amount, it is important that the samples contain RNA of high integrity (RIN > 6) and are devoid of contaminants (Nanodrop A260/A230 between 1.8 and 2.2).
  • BRB-seq is 3’-end RNA sequencing method and, as such, requires significantly less sequencing as compared to standard full-length RNA-seq in order to reach accurate gene quantification. We therefore normally recommend to sequence 4 to 5 million reads for each sample, which enables the reliable and unbiased detection of over 18’000 genes.
  • As part of our standard service pipeline, we align the generated data to the genome of choice, provide a detailed report on the alignment and gene counting statistics and, finally, provide ready-to-use gene count matrices for downstream analysis.

See our products

Our BRB-seq kit makes it possible to process virtually any number of RNA samples in one single tube, which helps reduce technical variation, turnaround times and costs. Experience sample to sequencing-ready library in one day.

Discover our kits
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BRB-seq was a great way for us to multiplex large numbers of RNA samples for sequencing. We wanted a gene expression curve with a tight temporal resolution, thus, many time points for several conditions. We are very happy with the quality of the results and with the cost benefit.

Dr. Nuno Miguel Luis, CNRS Researcher

BRB-seq is very cost-effective and it enables accurate comparison of RNA levels among a large number of samples.

Dr. Hirokazu Okada, ETH Zurich

BRB-seq allowed us to plan an experiment with ten times more replicates than usual, and gain insight into the variability and control of gene expression in fly embryogenesis

Prof. Dr. Marc Robinson-Rechavi, University of Lausanne

BRB-seq provided us with a cost-effective way to quantify transcriptomic changes from a large set of samples.

Prof. Dr. Martin Klingenspor, TUM Munich

Speak with our RNA sequencing experts

Need guidance or have questions about BRB-seq and our service?

Easily book a call with our BRB-seq experts.