MERCURIUS™

Cell Lysate BRB-seq Service

By leveraging BRB-seq, we not only provide to industrial and academic clients high quality RNA-seq data, but we also do so with the highest affordability and shortest turnaround times on the market.

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Benefits

The generated 3' mRNA-seq data is ideally suited for differential gene expression analysis

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Bulk RNA sequencing at scale

More samples, more replicates. Robust results, significant discoveries.

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Ideal for projects of all sizes

You can trust us. We have ran projects from as low as 6 samples to as many as 9,000.

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Fast turnaround times

From samples to data in as fast as 1.5 months.

What we do

MERCURIUS ™

Cell Lysate BRB-seq Service

The MERCURIUS™ Cell Lysate BRB-seq service offers the most comprehensive, high-throughput and cost-efficient solution for gene expression analysis projects starting from 2D cell cultures or organoids.

As part of the Cell Lysate service, we send our clients a proprietary Lysis Buffer which is optimized for both cell lysis and downstream enzymatic reactions, without having to perform RNA extraction in between.

After mixing the Lysis Buffer with their cells, the client can send back to us their cell lysates, which are then ready to be processed in the BRB-seq workflow.

By leveraging on the advantages the Cell Lysate BRB-seq system, such as cost-efficiency and high-throughput, we are able to offer the most competitive price and turnaround time on the market.

As a result of our service, our clients receive raw sequencing data (fastq files), gene count matrices and analysis reports files.

Optional downstream differential gene expression analysis are also available upon request.

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How does it work

  • Alithea ships the Cell Lysis Buffer

  • Client transfers the lysates to the provided well plate

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  • Client ships cell lysates to Alithea

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  • 1 week

    Incoming QC - Client Checkpoint

    (Nanodrop, Ribogreen, Tapestation)
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  • 2 days

    MERCURIUS™ Cell lysate BRB-seq for Illumina®

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  • 1 week

    Library QC - Client Checkpoint

    (Qubit, Fragment analyzer, shallow sequencing)
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  • 1.5 weeks

    Deep sequencing - Illumina

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  • 1 week

    Data analysis and reporting - Client Checkpoint

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  • Data

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  • Optional: Alithea performs Differential Gene Expression analysis​

FAQs

  • To guarantee high quality data, we normally request that each sample contains at least 200ng of total RNA in at least 10μl.
  • In addition to total RNA amount, it is important that the samples contain RNA of high integrity (RIN > 6) and are devoid of contaminants (Nanodrop A260/A230 between 1.8 and 2.2).
  • BRB-seq is 3’-end RNA sequencing method and, as such, requires significantly less sequencing as compared to standard full-length RNA-seq in order to reach accurate gene quantification. We therefore normally recommend to sequence 4 to 5 million reads for each sample, which enables the reliable and unbiased detection of over 18’000 genes.
  • As part of our standard service pipeline, we align the generated data to the genome of choice, provide a detailed report on the alignment and gene counting statistics and, finally, provide ready-to-use gene count matrices for downstream analysis.

See our products

Our BRB-seq kit makes it possible to process virtually any number of RNA samples in one single tube, which helps reduce technical variation, turnaround times and costs. Experience sample to sequencing-ready library in one day.

Discover our kits
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Speak with our RNA sequencing experts

Need guidance or have questions about BRB-seq and our service?

Easily book a call with our BRB-seq experts.