The generated 3' mRNA-seq data is ideally suited for differential gene expression analysis
Bulk RNA sequencing at scale
More samples, more replicates. Robust results, significant discoveries.
Ideal for projects of all sizes
You can trust us. We have ran projects from as low as 6 samples to as many as 9,000.
Fast turnaround times
From samples to data in as fast as 1.5 months.
What we do
Cell Lysate BRB-seq Service
The MERCURIUS™ Cell Lysate BRB-seq service offers the most comprehensive, high-throughput and cost-efficient solution for gene expression analysis projects starting from 2D cell cultures or organoids.
As part of the Cell Lysate service, we send our clients a proprietary Lysis Buffer which is optimized for both cell lysis and downstream enzymatic reactions, without having to perform RNA extraction in between.
After mixing the Lysis Buffer with their cells, the client can send back to us their cell lysates, which are then ready to be processed in the BRB-seq workflow.
By leveraging on the advantages the Cell Lysate BRB-seq system, such as cost-efficiency and high-throughput, we are able to offer the most competitive price and turnaround time on the market.
As a result of our service, our clients receive raw sequencing data (fastq files), gene count matrices and analysis reports files.
Optional downstream differential gene expression analysis are also available upon request.
How does it work
Alithea ships the Cell Lysis Buffer
Client transfers the lysates to the provided well plate
Client ships cell lysates to Alithea
Incoming QC - Client Checkpoint(Nanodrop, Ribogreen, Tapestation)
MERCURIUS™ Cell lysate BRB-seq for Illumina®
Library QC - Client Checkpoint(Qubit, Fragment analyzer, shallow sequencing)
Deep sequencing - Illumina
Data analysis and reporting - Client Checkpoint
Optional: Alithea performs Differential Gene Expression analysis
To guarantee high quality data, we normally request that each sample contains at least 200ng of total RNA in at least 10μl.
In addition to total RNA amount, it is important that the samples contain RNA of high integrity (RIN > 6) and are devoid of contaminants (Nanodrop A260/A230 between 1.8 and 2.2).
BRB-seq is 3’-end RNA sequencing method and, as such, requires significantly less sequencing as compared to standard full-length RNA-seq in order to reach accurate gene quantification. We therefore normally recommend to sequence 4 to 5 million reads for each sample, which enables the reliable and unbiased detection of over 18’000 genes.
As part of our standard service pipeline, we align the generated data to the genome of choice, provide a detailed report on the alignment and gene counting statistics and, finally, provide ready-to-use gene count matrices for downstream analysis.
Dr. Nuno Miguel Luis, CNRS Researcher
Dr. Hirokazu Okada, ETH Zurich
Prof. Dr. Marc Robinson-Rechavi, University of Lausanne
Prof. Dr. Martin Klingenspor, TUM Munich
Speak with our RNA sequencing experts
Need guidance or have questions about BRB-seq and our service?
Easily book a call with our BRB-seq experts.